Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1968 Jul;96(1):184–190. doi: 10.1128/jb.96.1.184-190.1968

Nucleotide Composition of Nuclear and Mitochondrial Deoxyribonucleic Acid of Fungi

Vicente D Villa 1, R Storck 1
PMCID: PMC252271  PMID: 5663568

Abstract

The buoyant density of nuclear and mitochondrial deoxyribonucleic acid (DNA) from 14 species of fungi was determined by CsCl density gradient equilibrium centrifugation. The buoyant density of both types of DNA was the same for all three Mucorales analyzed. The buoyant density of mitochondrial DNA was significantly lower than that of the nuclear DNA for nine species of Ascomycetes and two species of Basidiomycetes. No simple correlation could be obtained from the comparison of the two types of DNA. Mitochondrial DNA represented a very small proportion of total DNA. Heat-denatured mitochondrial DNA renatured more readily than nuclear DNA.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARTNICKI-GARCIA S., NICKERSON W. J. Nutrition, growth, and morphogenesis of Mucor rouxii. J Bacteriol. 1962 Oct;84:841–858. doi: 10.1128/jb.84.4.841-858.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CHENG T. Y., SUEOKA N. POLYMER SIMILAR TO POLYDEOXYADENYLATE-THYMIDYLATE IN VARIOUS TISSUES OF A MARINE CRAB. Science. 1964 Mar 27;143(3613):1442–1443. doi: 10.1126/science.143.3613.1442. [DOI] [PubMed] [Google Scholar]
  3. Corneo G., Moore C., Sanadi D. R., Grossman L. I., Marmur J. Mitochondrial DNA in yeast and some mammalian species. Science. 1966 Feb 11;151(3711):687–689. doi: 10.1126/science.151.3711.687. [DOI] [PubMed] [Google Scholar]
  4. Cummins J. E., Rusch H. P., Evans T. E. Nearest neighbor frequencies and the phylogenetic origin of mitochondrial DNA in Physarum polycephalum. J Mol Biol. 1967 Jan 28;23(2):281–284. doi: 10.1016/s0022-2836(67)80034-2. [DOI] [PubMed] [Google Scholar]
  5. Dawid I. B. Evidence for the mitochondrial origin of frog egg cytoplasmic DNA. Proc Natl Acad Sci U S A. 1966 Jul;56(1):269–276. doi: 10.1073/pnas.56.1.269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Edelman M., Epstein H. T., Schiff J. A. Isolation and characterization of DNA from the mitochondrial fraction of Euglena. J Mol Biol. 1966 Jun;17(2):463–469. doi: 10.1016/s0022-2836(66)80156-0. [DOI] [PubMed] [Google Scholar]
  7. Evans T. E. Synthesis of a cytoplasmic DNA during the G2 interphase of Physarum polycephalum. Biochem Biophys Res Commun. 1966 Mar 22;22(6):678–683. doi: 10.1016/0006-291x(66)90200-2. [DOI] [PubMed] [Google Scholar]
  8. Granick S., Gibor A. The DNA of chloroplasts, mitochondria and centrioles. Prog Nucleic Acid Res Mol Biol. 1967;6:143–186. doi: 10.1016/s0079-6603(08)60526-7. [DOI] [PubMed] [Google Scholar]
  9. Guttes E. W., Hanawalt P. C., Guttes S. Mitochondrial DNA synthesis and the mitotic cycle in Physarum polycephalum. Biochim Biophys Acta. 1967 Jun 20;142(1):181–194. doi: 10.1016/0005-2787(67)90526-6. [DOI] [PubMed] [Google Scholar]
  10. Haidle C. W., Storck R. Inhibition by cycloheximide of protein and RNA synthesis in Mucor rouxii. Biochem Biophys Res Commun. 1966 Jan 24;22(2):175–180. doi: 10.1016/0006-291x(66)90428-1. [DOI] [PubMed] [Google Scholar]
  11. Kalf G. F., Gréce M. A. The isolation of deoxyribonucleic acid from lamb heart mitochondria. J Biol Chem. 1966 Mar 10;241(5):1019–1022. [PubMed] [Google Scholar]
  12. Küntzel H., Noll H. Mitochondrial and cytoplasmic polysomes from Neurospora crassa. Nature. 1967 Sep 23;215(5108):1340–1345. doi: 10.1038/2151340a0. [DOI] [PubMed] [Google Scholar]
  13. LUCK D. J., REICH E. DNA IN MITOCHONDRIA OF NEUROSPORA CRASSA. Proc Natl Acad Sci U S A. 1964 Oct;52:931–938. doi: 10.1073/pnas.52.4.931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Meselson M., Stahl F. W., Vinograd J. EQUILIBRIUM SEDIMENTATION OF MACROMOLECULES IN DENSITY GRADIENTS. Proc Natl Acad Sci U S A. 1957 Jul 15;43(7):581–588. doi: 10.1073/pnas.43.7.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mounolou J. C., Jakob H., Slonimski P. P. Mitochondrial DNA from yeast "petite" mutants: specific changes in buoyant density corresponding to different cytoplasmic mutations. Biochem Biophys Res Commun. 1966 Jul 20;24(2):218–224. doi: 10.1016/0006-291x(66)90723-6. [DOI] [PubMed] [Google Scholar]
  16. Moustacchi E., Williamson D. H. Physiological variations in satellite components of yeast DNA detected by density gradient centrifugation. Biochem Biophys Res Commun. 1966 Apr 6;23(1):56–61. doi: 10.1016/0006-291x(66)90268-3. [DOI] [PubMed] [Google Scholar]
  17. Rabinowitz M., Sinclair J., DeSalle L., Haselkorn R., Swift H. H. Isolation of deoxyribonucleic acid from mitochondria of chick embryo heart and liver. Proc Natl Acad Sci U S A. 1965 May;53(5):1126–1133. doi: 10.1073/pnas.53.5.1126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Reich E., Luck D. J. Replication and inheritance of mitochondrial DNA. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1600–1608. doi: 10.1073/pnas.55.6.1600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rifkin M. R., Wood D. D., Luck D. J. Ribosomal RNA and ribosomes from mitochondria of Neurospora crassa. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1025–1032. doi: 10.1073/pnas.58.3.1025. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. SCHILDKRAUT C. L., MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol. 1962 Jun;4:430–443. doi: 10.1016/s0022-2836(62)80100-4. [DOI] [PubMed] [Google Scholar]
  21. Schneider W. C., Kuff E. L. The isolation and some properties of rat liver mitochondrial deoxyribonucleic acid. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1650–1658. doi: 10.1073/pnas.54.6.1650. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sinclair J. H., Stevens B. J. Circular DNA filaments from mouse mitochondria. Proc Natl Acad Sci U S A. 1966 Aug;56(2):508–514. doi: 10.1073/pnas.56.2.508. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sinclair J. H., Stevens B. J., Sanghavi P., Rabinowitz M. Mitochondrial-satellite and circular DNA filaments in yeast. Science. 1967 Jun 2;156(3779):1234–1237. doi: 10.1126/science.156.3779.1234. [DOI] [PubMed] [Google Scholar]
  24. Storck R. Nucleotide composition of nucleic acids of fungi. II. Deoxyribonucleic acids. J Bacteriol. 1966 Jan;91(1):227–230. doi: 10.1128/jb.91.1.227-230.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Suyama Y. Mitochondrial deoxyribonucleic acid of Tetrahymena. Its partial physical characterization. Biochemistry. 1966 Jul;5(7):2214–2221. doi: 10.1021/bi00871a009. [DOI] [PubMed] [Google Scholar]
  26. Suyama Y., Preer J. R., Jr Mitochondiral DNA from protozoa. Genetics. 1965 Nov;52(5):1051–1058. doi: 10.1093/genetics/52.5.1051. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. TAYLOR M. M., STORCK R. UNIQUENESS OF BACTERIAL RIBOSOMES. Proc Natl Acad Sci U S A. 1964 Oct;52:958–965. doi: 10.1073/pnas.52.4.958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Taylor M. M., Glasgow J. E., Storck R. Sedimentation coefficients of RNA from 70S and 80S ribosomes. Proc Natl Acad Sci U S A. 1967 Jan;57(1):164–169. doi: 10.1073/pnas.57.1.164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Tewari K. K., Jayaraman J., Mahler H. R. Separation and characterization of mitochondrial DNA from yeast. Biochem Biophys Res Commun. 1965 Oct 26;21(2):141–148. doi: 10.1016/0006-291x(65)90100-2. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES