Skip to main content

Some NLM-NCBI services and products are experiencing heavy traffic, which may affect performance and availability. We apologize for the inconvenience and appreciate your patience. For assistance, please contact our Help Desk at info@ncbi.nlm.nih.gov.

Journal of Bacteriology logoLink to Journal of Bacteriology
. 1968 Jul;96(1):205–214. doi: 10.1128/jb.96.1.205-214.1968

Isolation of High-Frequency Recombining Strains from Escherichia coli Containing the V Colicinogenic Factor

Phyllis L Khan 1
PMCID: PMC252274  PMID: 4874307

Abstract

The isolation and characterization of high-frequency recombining strains from different Escherichia coli host cells containing either the F factor or the Col V factor are described. The strains (with one exception) formed from three of the V+ parents showed the same origin and polarity of transfer (xyl-arg-pro-trp-his-mal). The Hfr strains formed from the one remaining V+ and the F+ host cells showed a greater variety in their points of origin. In addition, several Hfr strains isolated from V+ parents lost the ability to produce colicin V. Fv+ segregants of these were isolated, and the Fv factors appeared to retain their preferential site for Hfr formation, but they lacked other propertes controlled by the Col V factor. Chromosomal integration of episomes and its relation to the fertility of F+ and V+ strains are discussed. Production of colicin V appeared to be uninfluenced by the state of the Col V factor within the cell.

Full text

PDF
205

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERNSTEIN H. L. Fertility factors in Escherichia coli. Symp Soc Exp Biol. 1958;12:93–103. [PubMed] [Google Scholar]
  2. Beckwith J. R., Signer E. R., Epstein W. Transposition of the Lac region of E. coli. Cold Spring Harb Symp Quant Biol. 1966;31:393–401. doi: 10.1101/sqb.1966.031.01.051. [DOI] [PubMed] [Google Scholar]
  3. Beckwith J. R., Signer E. R. Transposition of the lac region of Escherichia coli. I. Inversion of the lac operon and transduction of lac by phi80. J Mol Biol. 1966 Aug;19(2):254–265. doi: 10.1016/s0022-2836(66)80003-7. [DOI] [PubMed] [Google Scholar]
  4. Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. A proposal for a uniform nomenclature in bacterial genetics. Genetics. 1966 Jul;54(1):61–76. doi: 10.1093/genetics/54.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FREDERICQ P. Colicins. Annu Rev Microbiol. 1957;11:7–22. doi: 10.1146/annurev.mi.11.100157.000255. [DOI] [PubMed] [Google Scholar]
  6. Herschman H. R., Helinski D. R. Comparative study of the events associated with colicin induction. J Bacteriol. 1967 Sep;94(3):691–699. doi: 10.1128/jb.94.3.691-699.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hickson F. T., Roth T. F., Helinski D. R. Circular DNA forms of a bacterial sex factor. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1731–1738. doi: 10.1073/pnas.58.4.1731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hirota Y. THE EFFECT OF ACRIDINE DYES ON MATING TYPE FACTORS IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1960 Jan;46(1):57–64. doi: 10.1073/pnas.46.1.57. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KAHN P., HELINSKI D. R. RELATIONSHIP BETWEEN COLICINOGENIC FACTORS E1 AND V AND AN F FACTOR IN ESCHERICHIA COLI. J Bacteriol. 1964 Dec;88:1573–1579. doi: 10.1128/jb.88.6.1573-1579.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kahn P. L., Helinski D. R. Interaction between colicinogenic factor V and the integrated F factor in an Hfr strain of Escherichia coli. J Bacteriol. 1965 Nov;90(5):1276–1282. doi: 10.1128/jb.90.5.1276-1282.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lederberg E M, Lederberg J. Genetic Studies of Lysogenicity in Escherichia Coli. Genetics. 1953 Jan;38(1):51–64. doi: 10.1093/genetics/38.1.51. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Matney T. S., Goldschmidt E. P., Erwin N. S., Scroggs R. A. A preliminary map of genomic sites for F-attachment in Escherichia coli K12. Biochem Biophys Res Commun. 1964 Oct 14;17(3):278–281. doi: 10.1016/0006-291x(64)90397-3. [DOI] [PubMed] [Google Scholar]
  13. Nagel De Zwaig R. Association between colicinogenic and fertility factors. Genetics. 1966 Aug;54(2):381–390. doi: 10.1093/genetics/54.2.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nomura M. Colicins and related bacteriocins. Annu Rev Microbiol. 1967;21:257–284. doi: 10.1146/annurev.mi.21.100167.001353. [DOI] [PubMed] [Google Scholar]
  15. Scaife J. Episomes. Annu Rev Microbiol. 1967;21:601–638. doi: 10.1146/annurev.mi.21.100167.003125. [DOI] [PubMed] [Google Scholar]
  16. Taylor A. L., Trotter C. D. Revised linkage map of Escherichia coli. Bacteriol Rev. 1967 Dec;31(4):332–353. doi: 10.1128/br.31.4.332-353.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES