Abstract
In addition to canthaxanthin, seven pigment fractions were isolated from Micrococcus roseus. They were purified by solvent partitioning and by column and thin-layer chromatography. Visible absorption spectra, chromatographic behavior, and partition coefficients of the pigments and derivatives prepared from the pigments were used in characterizing them. Both α- and β-carotene derivatives were present. The structure of one pigment was suggested as phoenicoxanthin (3-hydroxy-4,4′-diketo-β-carotene). Four other pigments were tentatively characterized as a dihydroxy-3,4-dehydro-α-carotene, a dihydroxy-α-carotene, a diketo-α-carotene, and a polyhydroxy-β-carotene. Two pigments were isolated in trace amounts and could not be characterized. All the pigments studied were isolated as mixtures of cis-trans isomers and all except the diketo-α-carotene were isolated as esters from M. roseus. Quantitation of the pigments showed that canthaxanthin (4,4′-diketo-β-carotene) represented 85% of the pigment recovered from extracts. Three of the other pigments contributed a significant proportion of the remaining pigments, whereas the other four were present in only small amounts. β-Carotene derivatives comprised 96% and α-carotene derivatives 4% of the pigments recovered from extracts.
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