Abstract
The model of pneumonia produced by intranasal inoculation of Mycoplasma pulmonis in gnotobiotic mice provided the opportunity to study the localization and identification of the infecting organisms in the tissues by immunofluorescence techniques. Frozen sections of pneumonic mouse lung were fixed in acetone, layered with rabbit anti-M. pulmonis serum, washed, layered again with fluorescein-isothiocyanate-labeled goat anti-rabbit globulin, washed again, and examined by fluorescence microscopy. A bright line of fluorescence was seen at the bronchial epithelial surface, usually in a continuous layer. Occasional masses of fluorescence were seen in the polymorphonuclear leukocytic exudate in the bronchial lumen. Sections of tissues fixed in Helle's or 10% Formalin fixatives and stained with hematoxylin and eosin were reviewed by light microscopy and revealed a zone of blue-staining material composed of tiny coccoid bodies in the same locations at the bronchial epithelial surface as in the immunofluorescent preparations and in previously reported electron microscope studies.
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- CLYDE W. A., Jr MYCOPLASMA SPECIES IDENTIFICATION BASED UPON GROWTH INHIBITION BY SPECIFIC ANTISERA. J Immunol. 1964 Jun;92:958–965. [PubMed] [Google Scholar]
- DAJANI A. S., CLYDE W. A., Jr, DENNY F. W. EXPERIMENTAL INFECTION WITH MYCOPLASMA PNEUMONIAE (EATON'S AGENT). J Exp Med. 1965 Jun 1;121:1071–1086. doi: 10.1084/jem.121.6.1071. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DONALD H. B., LIU C. Cytological studies of chick embryo cells infected with the virus of primary atypical pneumonia. Virology. 1959 Sep;9:20–29. doi: 10.1016/0042-6822(59)90097-2. [DOI] [PubMed] [Google Scholar]
- GOODBURN G. M., MARMION B. P. A study of the properties of Eaton's primary atypical pneumonia organism. J Gen Microbiol. 1962 Oct;29:271–290. doi: 10.1099/00221287-29-2-271. [DOI] [PubMed] [Google Scholar]
- HAYFLICK L., CHANOCK R. M. MYCOPLASMA SPECIES OF MAN. Bacteriol Rev. 1965 Jun;29:185–221. doi: 10.1128/br.29.2.185-221.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LIU C. Studies on primary atypical pneumonia. I. Localization, isolation, and cultivation of a virus in chick embryos. J Exp Med. 1957 Oct 1;106(4):455–466. doi: 10.1084/jem.106.4.455. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lutsky I. I., Organick A. B. Pneumonia due to mycoplasma in gnotobiotic mice. I. Pathogenicity of Mycoplasma pneumoniae, Mycoplasma salivarium, and Mycoplasma pulmonis for the lungs of conventional and gnotobiotic mice. J Bacteriol. 1966 Oct;92(4):1154–1163. doi: 10.1128/jb.92.4.1154-1163.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Organick A. B., Lutsky I. I. Pneumonia due to Mycoplasma in gnotobiotic mice. 3. Lesions in the lungs of gnotobiotic mice after multiple intranasal inoculations of broth cultures of Mycoplasma pneumoniae. J Bacteriol. 1968 Jun;95(6):2310–2316. doi: 10.1128/jb.95.6.2310-2316.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Organick A. B., Siegesmund K. A., Lutsky I. I. Pneumonia due to mycoplasma in gnotobiotic mice. II. Localization of Mycoplasma pulmonis in the lungs of infected gnotobiotic mice by electron microscopy. J Bacteriol. 1966 Oct;92(4):1164–1176. doi: 10.1128/jb.92.4.1164-1176.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]