Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1968 Oct;96(4):902–908. doi: 10.1128/jb.96.4.902-908.1968

Relation of Mucoid Growth of Staphylococcus aureus to Clumping Factor Reaction, Morphology in Serum-Soft Agar, and Virulence

Kosaku Yoshida a,1, Richard D Ekstedt a
PMCID: PMC252396  PMID: 5686016

Abstract

The growth characteristics of several strains of Staphylococcus aureus in Brain Heart Infusion and in a modified Staphylococcus Medium No. 110 were compared. In the latter medium all of the strains studied showed an increased mucoid character. Some of the strains studied showed a greater potential to synthesize excess slime layer material than others. The highly mucoid strains grew as diffuse-type colonies in modified Staphylococcus Medium No. 110 serum-soft agar and reacted as though they were negative in the test for clumping factor. These strains were also found to be more virulent when used to challenge normal mice intraperitoneally.

Full text

PDF
902

Images in this article

904Image
on p.904
904Image
on p.904
905Image
on p.905

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. COHN Z. A. Determinants of infection in the peritoneal cavity. I. Response to and fate of Staphylococcus aureus and Staphylococcus albus in the mouse. Yale J Biol Med. 1962 Aug;35:12–28. [PMC free article] [PubMed] [Google Scholar]
  2. Ekstedt R. D. Mechanisms of resistance to staphylococcal infection: natural and acquired. Ann N Y Acad Sci. 1965 Jul 23;128(1):301–334. doi: 10.1111/j.1749-6632.1965.tb11646.x. [DOI] [PubMed] [Google Scholar]
  3. Ekstedt R. D. Studies on immunity to staphylococcal infection in mice. IV. The role of specific and nonspecific immunity. J Infect Dis. 1966 Oct;116(4):514–522. doi: 10.1093/infdis/116.4.514. [DOI] [PubMed] [Google Scholar]
  4. FINKELSTEIN R. A., SULKIN S. E. Characteristics of coagulase positive and coagulase negative staphylococci in serum-soft agar. J Bacteriol. 1958 Mar;75(3):339–344. doi: 10.1128/jb.75.3.339-344.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FISHER M. W., DEVLIN H. B., ERLANDSON A. L. A NEW STAPHYLOCOCCAL ANTIGEN. ITS PREPARATION AND IMMUNIZING ACTIVITY AGAINST EXPERIMENTAL INFECTIONS. Nature. 1963 Sep 14;199:1074–1075. doi: 10.1038/1991074a0. [DOI] [PubMed] [Google Scholar]
  6. FISHER S. A heat stable protective staphylococcal antigen. Aust J Exp Biol Med Sci. 1960 Dec;38:479–485. doi: 10.1038/icb.1960.53. [DOI] [PubMed] [Google Scholar]
  7. HUNT G. A., MOSES A. J. Acute infection of mice with Smith strain of Staphylococcus aureus. Science. 1958 Dec 19;128(3338):1574–1575. doi: 10.1126/science.128.3338.1574. [DOI] [PubMed] [Google Scholar]
  8. KOENIG M. G. Factors relating to the virulence of staphylococci. I. Comparative studies on two colonial variants. Yale J Biol Med. 1962 Jun;34:537–559. [PMC free article] [PubMed] [Google Scholar]
  9. KOENIG M. G., MELLY M. A., ROGERS D. E. Factors relating to the virulence of Staphylococci. III. Antibacterial versus antioxic immunity. J Exp Med. 1962 Nov 1;116:601–610. doi: 10.1084/jem.116.5.601. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Koenig M. G., Melly M. A. The importance of surface antigens in staphylococcal virulence. Ann N Y Acad Sci. 1965 Jul 23;128(1):231–250. doi: 10.1111/j.1749-6632.1965.tb11641.x. [DOI] [PubMed] [Google Scholar]
  11. MORSE S. I. Isolation and properties of a surface antigen of Staphylococcus aureus. J Exp Med. 1962 Feb 1;115:295–311. doi: 10.1084/jem.115.2.295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mudd S. Capsulation, pseudocapsulation, and the somatic antigens of the surface of Staphylococcus aureus. Ann N Y Acad Sci. 1965 Jul 23;128(1):45–58. doi: 10.1111/j.1749-6632.1965.tb11628.x. [DOI] [PubMed] [Google Scholar]
  13. Parker S. E., Warner P., Slipetz W. K. Estimation of virulence of Staphylococcus aureus for mice: the exceptional virulence of the Smith strain. Can J Microbiol. 1966 Aug;12(4):753–762. doi: 10.1139/m66-102. [DOI] [PubMed] [Google Scholar]
  14. SALL T., MUDD S., TAUBLER J. Concerning the surfaces of cells of Staphylococcus pyogenes. I. A pseudocapsulation phenomenon under certain experimental conditions. J Exp Med. 1961 Apr 1;113:693–700. doi: 10.1084/jem.113.4.693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. WILEY B. B. A new virulence test for Staphylococcus aureus and its application to encapsulated strains. Can J Microbiol. 1961 Dec;7:933–943. doi: 10.1139/m61-118. [DOI] [PubMed] [Google Scholar]
  16. Warner P., Slipetz W. K., Kroeker J. Mouse-protective antibodies in human sera against the Smith strain of Staphylococcus aureus. Can J Microbiol. 1966 Oct;12(5):949–955. doi: 10.1139/m66-128. [DOI] [PubMed] [Google Scholar]
  17. Wiley B. B., Maverakis N. H. Virulent and avirulent encapsulated variants of Staphyococcus aureus. J Bacteriol. 1968 Mar;95(3):998–1002. doi: 10.1128/jb.95.3.998-1002.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES