Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1968 Oct;96(4):950–957. doi: 10.1128/jb.96.4.950-957.1968

Transport of Aminophosphonic Acids in Lactobacillus plantarum and Streptococcus faecalis

Joseph T Holden 1, Josephus N A Van Balgooy 1, James S Kittredge 1
PMCID: PMC252403  PMID: 4971894

Abstract

Aminophosphonic acids analogous to glutamic acid, aspartic acid, alanine, and valine were actively accumulated by Lactobacillus plantarum. Uptake was dependent on the availability of glucose and, in all cases, the estimated intracellular concentrations substantially exceeded extracellular levels. During uptake, there was little metabolism of tritiated 2-amino-3-phosphonopropionic acid (APP), the aspartic acid analogue, and a negligible incorporation of isotope from this substance into the nucleic acid, lipid, protein, or cell wall fractions of the cell. Competition studies with APP indicated that its transport in L. plantarum and in Streptococcus faecalis was antagonized only by structurally related compounds such as glutamic, aspartic, and cysteic acids. Kinetic studies showed that APP was taken up by a single catalytic system in S. faecalis. A mutant strain of this organism which lacks one of two kinetically distinguishable dicarboxylic amino acid transport systems failed to accumulate measurable amounts of APP. These experiments indicate that the aminophosphonic acids are accumulated by the amino acid transport systems in these bacteria with minimal metabolic changes.

Full text

PDF
950

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRUNI A., LUCIANI S., CONTESSA A. R. INHIBITION BY ATRACTYLOSIDE OF THE BINDING OF ADENINE-NUCLEOTIDES TO RAT-LIVER MITOCHONDRIA. Nature. 1964 Mar 21;201:1219–1220. doi: 10.1038/2011219a0. [DOI] [PubMed] [Google Scholar]
  2. Brierley G., O'Brien R. L. Compartmentation of heart mitochondria. II. Mitochondrial adenine nucleotides and the action of atractyloside. J Biol Chem. 1965 Nov;240(11):4532–4539. [PubMed] [Google Scholar]
  3. Duée E. D., Vignais P. V. Atractyloside-sensitive translocation of phosphonic acid analogues of adenine nucleotides in mitochondria. Biochem Biophys Res Commun. 1968 Feb 26;30(4):420–427. doi: 10.1016/0006-291x(68)90761-4. [DOI] [PubMed] [Google Scholar]
  4. FRAENKEL D. G., FALCOZ-KELLY F., HORECKER B. L. THE UTILIZATION OF GLUCOSE 6-PHOSPHATE BY GLUCOKINASELESS AND WILD-TYPE STRAINS OF ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1964 Nov;52:1207–1213. doi: 10.1073/pnas.52.5.1207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HAYASHI S., KOCH J. P., LIN E. C. ACTIVE TRANSPORT OF L-ALPHA-GLYCEROPHOSPHATE IN ESCHERICHIA COLI. J Biol Chem. 1964 Sep;239:3098–3105. [PubMed] [Google Scholar]
  6. HOLDEN J. T. DEPENDENCE OF AMINO ACID TRANSPORT AND ACCUMULATION ON OSMOTIC FACTORS IN VITAMIN B6-DEFICIENT LACTOBACILLUS ARABINOSUS. Biochim Biophys Acta. 1963 Aug 13;74:401–417. doi: 10.1016/0006-3002(63)91384-2. [DOI] [PubMed] [Google Scholar]
  7. HOLDEN J. T., HOLMAN J. Accumulation of freely extractable glutamic acid by lactic acid bacteria. J Biol Chem. 1959 Apr;234(4):865–871. [PubMed] [Google Scholar]
  8. HOLDEN J. T., WILDMAN R. B., SNELL E. E. Growth promotion by keto and hydroxy acids and its relation to vitamin B6. J Biol Chem. 1951 Aug;191(2):559–576. [PubMed] [Google Scholar]
  9. HORIGUCHI M., KANDATSU M. Isolation of 2-aminoethane phosphonic acid from rumen protozoa. Nature. 1959 Sep 19;184(Suppl 12):901–902. doi: 10.1038/184901b0. [DOI] [PubMed] [Google Scholar]
  10. Harkness D. R. Bacterial growth on aminoalkylphosphonic acids. J Bacteriol. 1966 Sep;92(3):623–627. doi: 10.1128/jb.92.3.623-627.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. KITTREDGE J. S., ROBERTS E., SIMONSEN D. G. The occurrence of free 2-aminoethylphosphonic acid in the sea anemone, Anthopleura elegantissima. Biochemistry. 1962 Jul;1:624–628. doi: 10.1021/bi00910a013. [DOI] [PubMed] [Google Scholar]
  12. LIN E. C., KOCH J. P., CHUSED T. M., JORGENSEN S. E. Utilization of L-alpha-glycerophosphate by Escherichia coli without hydrolysis. Proc Natl Acad Sci U S A. 1962 Dec 15;48:2145–2150. doi: 10.1073/pnas.48.12.2145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. MASTALERZ P., WIECZOREK Z., KOCHMAN M. UTILIZATION OF CARBON-BOUND PHOSPHORUS BY MICROORGANISMS. Acta Biochim Pol. 1965;12:151–156. [PubMed] [Google Scholar]
  14. Pfaff E., Klingenberg M., Heldt H. W. Unspecific permeation and specific exchange of adenine nucleotides in liver mitochondria. Biochim Biophys Acta. 1965 Jun 15;104(1):312–315. doi: 10.1016/0304-4165(65)90258-8. [DOI] [PubMed] [Google Scholar]
  15. Pogell B. M., Maity B. R., Frumkin S., Shapiro S. Induction of an active transport system for glucose 6-phosphate in Escherichia coli. Arch Biochem Biophys. 1966 Sep 26;116(1):406–415. doi: 10.1016/0003-9861(66)90047-6. [DOI] [PubMed] [Google Scholar]
  16. Rosenberg H., La Nauze J. M. The metabolism of phosphonates by microorganisms. The transport of aminoethylphosphonic acid in Bacillus cereus. Biochim Biophys Acta. 1967 Jun 13;141(1):79–90. doi: 10.1016/0304-4165(67)90247-4. [DOI] [PubMed] [Google Scholar]
  17. ZELEZNICK L. D., MYERS T. C., TITCHENER E. B. GROWTH OF ESCHERICHIA COLI ON METHYL- AND ETHYLPHOSPHONIC ACIDS. Biochim Biophys Acta. 1963 Nov 15;78:546–547. doi: 10.1016/0006-3002(63)90921-1. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES