Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1968 Oct;96(4):970–974. doi: 10.1128/jb.96.4.970-974.1968

Role of Relative Humidity in the Survival of Airborne Mycoplasma pneumoniae

D N Wright 1,2, G D Bailey 1,2, M T Hatch 1,2
PMCID: PMC252406  PMID: 5686020

Abstract

Aerosols of Mycoplasma pneumoniae were studied at several relative humidities at a controlled temperature of 27 C. Production of an experimentally reproducible aerosol required preatomization of the organism in its suspending fluid and was dependent on the type of fluid used in atomization as well as on the procedures used to produce an aerosol. The airborne particles studied were within the range of epidemiological significance, with most being 2 μm or less in diameter. Survival of the airborne mycoplasma in these particles was found to be best at very low and at very high humidities. The most lethal relative humidity levels were at 60 and 80%, at which levels fewer than 1% of the organisms survived over a 4-hr observation period. However, survival of the organism at most relative humidity levels was such that long-term infectivity could be expected from aerosols of M. pneumoniae. Because of the extreme sensitivity of M. pneumoniae at critical humidity levels, control of the airborne transmission of these organisms may be possible in selected spaces.

Full text

PDF
970

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSEN A. A. New sampler for the collection, sizing, and enumeration of viable airborne particles. J Bacteriol. 1958 Nov;76(5):471–484. doi: 10.1128/jb.76.5.471-484.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CHANOCK R. M., HAYFLICK L., BARILE M. F. Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO. Proc Natl Acad Sci U S A. 1962 Jan 15;48:41–49. doi: 10.1073/pnas.48.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CHANOCK R. M., MUFSON M. A., BLOOM H. H., JAMES W. D., FOX H. H., KINGSTON J. R. Eaton agent pneumonia. JAMA. 1961 Jan 21;175:213–220. doi: 10.1001/jama.1961.03040030037007. [DOI] [PubMed] [Google Scholar]
  4. Chanock R. M., Fox H. H., James W. D., Gutekunst R. R., White R. J., Senterfit L. B. Epidemiology of M. pneumoniae infection in military recruits. Ann N Y Acad Sci. 1967 Jul 28;143(1):484–496. doi: 10.1111/j.1749-6632.1967.tb27692.x. [DOI] [PubMed] [Google Scholar]
  5. Cox C. S. The aerosol survival of Escherichia coli JEPP sprayed from protecting agents into nitrogen atmospheres under changing relative humidity conditions. J Gen Microbiol. 1967 Oct;49(1):109–114. doi: 10.1099/00221287-49-1-109. [DOI] [PubMed] [Google Scholar]
  6. Cox C. S. The survival of Escherichia coli sprayed into air and into nitrogen from distilled water and from solutions of protecting agents, as a function of relative humidity. J Gen Microbiol. 1966 Jun;43(3):383–399. doi: 10.1099/00221287-43-3-383. [DOI] [PubMed] [Google Scholar]
  7. DIMMICK R. L. Characteristics of dried Serratia marcescens in the airborne state. J Bacteriol. 1960 Sep;80:289–296. doi: 10.1128/jb.80.3.289-296.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dowdle W. R., Stewart J. A., Heyward J. T., Robinson R. Q. Mycoplasma pneumoniae infections in a children's population: a five-year study. Am J Epidemiol. 1967 Jan;85(1):137–146. doi: 10.1093/oxfordjournals.aje.a120670. [DOI] [PubMed] [Google Scholar]
  9. FORSYTH B. R., BLOOM H. H., JOHNSON K. M., CHANOCK R. M. ETIOLOGY OF PRIMARY ATYPICAL PNEUMONIA IN A MILITARY POPULATION. JAMA. 1965 Feb 1;191:364–368. doi: 10.1001/jama.1965.03080050010002. [DOI] [PubMed] [Google Scholar]
  10. Foy H. M., Grayston J. T., Kenny G. E., Alexander E. R., McMahan R. Epidemiology of Mycoplasma pneumoniae infection in families. JAMA. 1966 Sep 12;197(11):859–866. [PubMed] [Google Scholar]
  11. GOLDBERG L. J., WATKINS H. M., BOERKE E. E., CHATIGNY M. A. The use of a rotating drum for the study of aerosols over extended periods of time. Am J Hyg. 1958 Jul;68(1):85–93. doi: 10.1093/oxfordjournals.aje.a119954. [DOI] [PubMed] [Google Scholar]
  12. GRAYSTON J. T., ALEXANDER E. R., KENNY G. E., CLARKE E. R., FREMONT J. C., MACCOLL W. A. MYCOPLASMA PNEUMONIAE INFECTIONS. CLINICAL AND EPIDEMIOLOGIC STUDIES. JAMA. 1965 Feb 1;191:369–374. doi: 10.1001/jama.1965.03080050015004. [DOI] [PubMed] [Google Scholar]
  13. Hottle G. A., Wright D. N. Growth and survival of Mycoplasma neurolyticum in liquid media. J Bacteriol. 1966 May;91(5):1834–1839. doi: 10.1128/jb.91.5.1834-1839.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. KELTON W. H. STORAGE OF MYCOPLASMA STRAINS. J Bacteriol. 1964 Mar;87:588–592. doi: 10.1128/jb.87.3.588-592.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kim K. S., Clyde W. A., Jr, Denny F. W. Physical properties of human Mycoplasma species. J Bacteriol. 1966 Jul;92(1):214–219. doi: 10.1128/jb.92.1.214-219.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kundsin R. B. Aerosols of mycoplasmas, L forms, and bacteria: comparison of particle size, viability, and lethality of ultraviolet radiation. Appl Microbiol. 1968 Jan;16(1):143–146. doi: 10.1128/am.16.1.143-146.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mufson M. A., Chang V., Gill V., Wood S. C., Romansky M. J., Chanock R. M. The role of viruses, mycoplasmas and bacteria in acute pneumonia in civilian adults. Am J Epidemiol. 1967 Nov;86(3):526–544. doi: 10.1093/oxfordjournals.aje.a120763. [DOI] [PubMed] [Google Scholar]
  18. Saliba G. S., Glezen W. P., Chin T. D. Mycoplasma pneumoniae infection in a resident boys' home. Am J Epidemiol. 1967 Sep;86(2):408–418. doi: 10.1093/oxfordjournals.aje.a120751. [DOI] [PubMed] [Google Scholar]
  19. Wright D. N., Bailey G. D., Hatch M. T. Survival of airborne Mycoplasma as affected by relative humidity. J Bacteriol. 1968 Jan;95(1):251–252. doi: 10.1128/jb.95.1.251-252.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES