Abstract
Acyl carrier protein (ACP coli) was isolated from commercially grown Escherichia coli B and was acetylated by chemical methods. Biological activity of the synthesized acetyl-ACP coli was checked in an in vitro fatty acid-synthesizing system isolated from E. coli B. Since acetyl-ACP is preferred over acetyl-coenzyme A (CoA) as a substrate in these reactions, the possibility that it may substitute for acetyl-CoA in biosynthetically and oxidatively important cellular pathways (glyoxylate and Krebs cycles, respectively) was examined. Acetyl-ACP was tested for substrate activity with the enzyme of each cycle which has been found to utilize acetyl-CoA. Crystalline citrate synthase (EC 4.1.3.7) of porcine origin (Calbiochem) was found to be inactive with acetyl-ACP coli, which acted neither as a substrate nor as an inhibitor in the presence of acetyl-CoA. Malate synthase (EC 4.1.3.2) of the acetate type was isolated from acetate-grown cells of a mutant of E. coli K-12 (VGD3H5) and was also found to be inactive with acetyl-ACP coli. The significance of these results and of the recent discovery of another phospho-pantetheine-containing protein are discussed.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ALBERTS A. W., MAJERUS P. W., TALAMO B., VAGELOS P. R. ACYL-CARRIER PROTEIN. II. INTERMEDIARY REACTIONS OF FATTY ACID SYNTHESIS. Biochemistry. 1964 Oct;3:1563–1571. doi: 10.1021/bi00898a030. [DOI] [PubMed] [Google Scholar]
- Ailhaud G. P., Vagelos P. R., Goldfine H. Involvement of acyl carrier protein in acylation of glycerol 3-phosphate in Clostridium butyricum. I. Purification of Clostridium butyricum acyl carrier protein and synthesis of long chain acyl derivatives of acyl carrier protein. J Biol Chem. 1967 Oct 10;242(19):4459–4465. [PubMed] [Google Scholar]
- Alberts A. W., Vagelos P. R. Acyl carrier protein. 8. Studies of acyl carrier protein and coenzyme A in Escherichia coli pantothenate or betaalanine auxotrophs. J Biol Chem. 1966 Nov 25;241(22):5201–5204. [PubMed] [Google Scholar]
- FALMAGNE P., VANDERWINKEL E., WIAME J. M. MISE EN EVIDENCE DE DEUX MALATE SYNTHASES CHEZ ESCHERICHIA COLI. Biochim Biophys Acta. 1965 May 18;99:246–258. [PubMed] [Google Scholar]
- Kass L. R., Bloch K. On the enzymatic synthesis of unsaturated fatty acids in Escherichia coli. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1168–1173. doi: 10.1073/pnas.58.3.1168. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MAJERUS P. W., ALBERTS A. W., VAGELOS P. R. THE ACYL CARRIER PROTEIN OF FATTY ACID SYNTHESIS: PURIFICATION, PHYSICAL PROPERTIES, AND SUBSTRATE BINDING SITE. Proc Natl Acad Sci U S A. 1964 Jun;51:1231–1238. doi: 10.1073/pnas.51.6.1231. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Matsumura S. Isolation and characterization of a protein containing 4'-phosphopantetheine from E. coli K-12. Biochem Biophys Res Commun. 1967 Nov 17;29(3):437–443. doi: 10.1016/0006-291x(67)90476-7. [DOI] [PubMed] [Google Scholar]
- Nagai S., Flavin M. Acetylhomoserine. An intermediate in the fungal biosynthesis of methionine. J Biol Chem. 1967 Sep 10;242(17):3884–3895. [PubMed] [Google Scholar]
- Rudney H., Stewart P. R., Majerus P. W., Vagelos P. R. The biosynthesis of beta-hydroxy-beta-methylglutaryl coenzyme A in yeast. V. The role of acyl carrier protein. J Biol Chem. 1966 Mar 10;241(5):1226–1228. [PubMed] [Google Scholar]