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. 1985 Oct;56(1):221–226. doi: 10.1128/jvi.56.1.221-226.1985

Influenza C virus RNA 7 codes for a nonstructural protein.

S Nakada, P N Graves, U Desselberger, R S Creager, M Krystal, P Palese
PMCID: PMC252509  PMID: 4032535

Abstract

The complete nucleotide sequence of RNA segment 7 of influenza C/California/78 virus was determined by using cloned cDNA derived from viral RNA. The gene is 934 nucleotides long and possesses a long open reading frame which can code for a protein of 286 amino acids. Hybrid arrest translation experiments with the cloned cDNA fragment and poly(A)-containing RNA isolated from virus-infected cells showed that a 28,500-molecular-weight protein is coded for by RNA 7. Comparison of the proteins induced in the cell-free system and in virus-infected cells with those found in purified virus suggests that the 28,500-molecular-weight protein is a nonstructural protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baez M., Taussig R., Zazra J. J., Young J. F., Palese P., Reisfeld A., Skalka A. M. Complete nucleotide sequence of the influenza A/PR/8/34 virus NS gene and comparison with the NS genes of the A/Udorn/72 and A/FPV/Rostock/34 strains. Nucleic Acids Res. 1980 Dec 11;8(23):5845–5858. doi: 10.1093/nar/8.23.5845. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Briedis D. J., Lamb R. A. Influenza B virus genome: sequences and structural organization of RNA segment 8 and the mRNAs coding for the NS1 and NS2 proteins. J Virol. 1982 Apr;42(1):186–193. doi: 10.1128/jvi.42.1.186-193.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  4. Compans R. W., Bishop D. H., Meier-Ewert H. Structural components of influenza C virions. J Virol. 1977 Feb;21(2):658–665. doi: 10.1128/jvi.21.2.658-665.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Desselberger U., Racaniello V. R., Zazra J. J., Palese P. The 3' and 5'-terminal sequences of influenza A, B and C virus RNA segments are highly conserved and show partial inverted complementarity. Gene. 1980 Feb;8(3):315–328. doi: 10.1016/0378-1119(80)90007-4. [DOI] [PubMed] [Google Scholar]
  6. Etkind P. R., Krug R. M. Purification of influenza viral complementary RNA: its genetic content and activity in wheat germ cell-free extracts. J Virol. 1975 Dec;16(6):1464–1475. doi: 10.1128/jvi.16.6.1464-1475.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hopp T. P., Woods K. R. Prediction of protein antigenic determinants from amino acid sequences. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3824–3828. doi: 10.1073/pnas.78.6.3824. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kendal A. P. A comparison of "influenza C" with prototype myxoviruses: receptor-destroycing activity (neuraminidase) and structural polypeptides. Virology. 1975 May;65(1):87–99. doi: 10.1016/0042-6822(75)90009-4. [DOI] [PubMed] [Google Scholar]
  9. Krystal M., Elliott R. M., Benz E. W., Jr, Young J. F., Palese P. Evolution of influenza A and B viruses: conservation of structural features in the hemagglutinin genes. Proc Natl Acad Sci U S A. 1982 Aug;79(15):4800–4804. doi: 10.1073/pnas.79.15.4800. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Krystal M., Young J. F., Palese P., Wilson I. A., Skehel J. J., Wiley D. C. Sequential mutations in hemagglutinins of influenza B virus isolates: definition of antigenic domains. Proc Natl Acad Sci U S A. 1983 Jul;80(14):4527–4531. doi: 10.1073/pnas.80.14.4527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lamb R. A., Choppin P. W. Segment 8 of the influenza virus genome is unique in coding for two polypeptides. Proc Natl Acad Sci U S A. 1979 Oct;76(10):4908–4912. doi: 10.1073/pnas.76.10.4908. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lamb R. A., Lai C. J. Sequence of interrupted and uninterrupted mRNAs and cloned DNA coding for the two overlapping nonstructural proteins of influenza virus. Cell. 1980 Sep;21(2):475–485. doi: 10.1016/0092-8674(80)90484-5. [DOI] [PubMed] [Google Scholar]
  13. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  14. Nakada S., Creager R. S., Krystal M., Aaronson R. P., Palese P. Influenza C virus hemagglutinin: comparison with influenza A and B virus hemagglutinins. J Virol. 1984 Apr;50(1):118–124. doi: 10.1128/jvi.50.1.118-124.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nakada S., Creager R. S., Krystal M., Palese P. Complete nucleotide sequence of the influenza C/California/78 virus nucleoprotein gene. Virus Res. 1984 Sep;1(6):433–441. doi: 10.1016/0168-1702(84)90001-7. [DOI] [PubMed] [Google Scholar]
  16. Palese P., Racaniello V. R., Desselberger U., Young J., Baez M. Genetic structure and genetic variation of influenza viruses. Philos Trans R Soc Lond B Biol Sci. 1980 Feb 25;288(1029):299–305. doi: 10.1098/rstb.1980.0005. [DOI] [PubMed] [Google Scholar]
  17. Pfeifer J. B., Compans R. W. Structure of the influenza C glycoprotein gene as determined from cloned DNA. Virus Res. 1984;1(4):281–296. doi: 10.1016/0168-1702(84)90017-0. [DOI] [PubMed] [Google Scholar]
  18. Ritchey M. B., Palese P., Kilbourne E. D. RNAs of influenza A, B, and C viruses. J Virol. 1976 May;18(2):738–744. doi: 10.1128/jvi.18.2.738-744.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ritchey M. B., Palese P., Schulman J. L. Mapping of the influenza virus genome. III. Identification of genes coding for nucleoprotein, membrane protein, and nonstructural protein. J Virol. 1976 Oct;20(1):307–313. doi: 10.1128/jvi.20.1.307-313.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Robertson J. S., Schubert M., Lazzarini R. A. Polyadenylation sites for influenza virus mRNA. J Virol. 1981 Apr;38(1):157–163. doi: 10.1128/jvi.38.1.157-163.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Staden R. A strategy of DNA sequencing employing computer programs. Nucleic Acids Res. 1979 Jun 11;6(7):2601–2610. doi: 10.1093/nar/6.7.2601. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Staden R. Further procedures for sequence analysis by computer. Nucleic Acids Res. 1978 Mar;5(3):1013–1016. doi: 10.1093/nar/5.3.1013. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Staden R. Sequence data handling by computer. Nucleic Acids Res. 1977 Nov;4(11):4037–4051. doi: 10.1093/nar/4.11.4037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wang L. H., Feldman R., Shibuya M., Hanafusa H., Notter M. F., Balduzzi P. C. Genetic structure, transforming sequence, and gene product of avian sarcoma virus UR1. J Virol. 1981 Oct;40(1):258–267. doi: 10.1128/jvi.40.1.258-267.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Yokota M., Nakamura K., Sugawara K., Homma M. The synthesis of polypeptides in influenza C virus-infected cells. Virology. 1983 Oct 15;130(1):105–117. doi: 10.1016/0042-6822(83)90121-6. [DOI] [PubMed] [Google Scholar]
  27. Young J. F., Palese P. Evolution of human influenza A viruses in nature: recombination contributes to genetic variation of H1N1 strains. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6547–6551. doi: 10.1073/pnas.76.12.6547. [DOI] [PMC free article] [PubMed] [Google Scholar]

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