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. 1985 Oct;56(1):298–302. doi: 10.1128/jvi.56.1.298-302.1985

Proteins associated with human parainfluenza virus type 3.

R C Jambou, N Elango, S Venkatesan
PMCID: PMC252530  PMID: 2993658

Abstract

The polypeptides associated with human parainfluenza virus type 3 were identified. Five proteins were present in detergent- and salt-resistant viral cores. Of these, three proteins designated NP0, NP1, and NP2 of 68,000, 58,000, and 52,000 daltons, respectively, were stably associated with 50S RNA in CsCl gradient-purified nucleocapsids. The amounts of NP1 and NP2 were variable, and these proteins were shown to be structurally related to the major nucleocapsid protein (NP0) by partial Staphylococcus aureus V8 protease mapping. The other core proteins included a 240K protein designated L (candidate for the viral polymerase) and an 84K protein designated as the phosphoprotein (P) on the basis of a predominant incorporation of Pi. The viral envelope had four prominent proteins (72, 53, 40, and 12K) under reducing conditions of electrophoresis. The 72 and 53K proteins were specifically labeled with [3H]glucosamine and [3H]mannose. When sulfhydryl reagents were removed, a new 62K protein was visualized in place of the 72, 53, and 12K proteins. The 53 and 12K proteins were interpreted to be the two subunits (F1 and F2) of the fusion protein, and the 72K protein was designated as the HN (hemagglutinin-neuraminidase) glycoprotein. The unglycosylated 40K protein represented the viral matrix protein (M). Immunoprecipitation of infected cell lysates with rabbit hyperimmune antiserum against purified virus confirmed the viral origin of these polypeptides.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Birrer M. J., Bloom B. R., Udem S. Characterization of measles polypeptides by monoclonal antibodies. Virology. 1981 Jan 30;108(2):381–390. doi: 10.1016/0042-6822(81)90446-3. [DOI] [PubMed] [Google Scholar]
  2. CHANOCK R. M. Association of a new type of cytopathogenic myxovirus with infantile croup. J Exp Med. 1956 Oct 1;104(4):555–576. doi: 10.1084/jem.104.4.555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chinchar V. G., Portner A. Functions of Sendai virus nucleocapsid polypeptides: enzymatic activities in nucleocapsids following cleavage of polypeptide P by Staphylococcus aureus protease V8. Virology. 1981 Feb;109(1):59–71. doi: 10.1016/0042-6822(81)90471-2. [DOI] [PubMed] [Google Scholar]
  4. Collins P. L., Hightower L. E., Ball L. A. Transcriptional map for Newcastle disease virus. J Virol. 1980 Sep;35(3):682–693. doi: 10.1128/jvi.35.3.682-693.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Elango N., Satake M., Coligan J. E., Norrby E., Camargo E., Venkatesan S. Respiratory syncytial virus fusion glycoprotein: nucleotide sequence of mRNA, identification of cleavage activation site and amino acid sequence of N-terminus of F1 subunit. Nucleic Acids Res. 1985 Mar 11;13(5):1559–1574. doi: 10.1093/nar/13.5.1559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Etkind P. R., Cross R. K., Lamb R. A., Merz D. C., Choppin P. W. In vitro synthesis of structural and nonstructural proteins of Sendai and SV5 viruses. Virology. 1980 Jan 15;100(1):22–33. doi: 10.1016/0042-6822(80)90548-6. [DOI] [PubMed] [Google Scholar]
  7. Giorgi C., Blumberg B. M., Kolakofsky D. Sendai virus contains overlapping genes expressed from a single mRNA. Cell. 1983 Dec;35(3 Pt 2):829–836. doi: 10.1016/0092-8674(83)90115-0. [DOI] [PubMed] [Google Scholar]
  8. Giuffre R. M., Tovell D. R., Kay C. M., Tyrrell D. L. Evidence for an interaction between the membrane protein of a paramyxovirus and actin. J Virol. 1982 Jun;42(3):963–968. doi: 10.1128/jvi.42.3.963-968.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Goswami K. K., Russell W. C. A comparison of paramyxoviruses by immunoprecipitation. J Gen Virol. 1982 May;60(Pt 1):177–183. doi: 10.1099/0022-1317-60-1-177. [DOI] [PubMed] [Google Scholar]
  10. Goswami K. K., Russell W. C. Monoclonal antibodies against human paramyxovirus type 3 and against SV5 virus: preparation and preliminary characterization. J Gen Virol. 1983 Aug;64(Pt 8):1663–1672. doi: 10.1099/0022-1317-64-8-1663. [DOI] [PubMed] [Google Scholar]
  11. Guskey L. E., Bergtrom G. High yield growth and purification of human parainfluenza type 3 virus and initial analysis of viral structural proteins. J Gen Virol. 1981 May;54(Pt 1):115–123. doi: 10.1099/0022-1317-54-1-115. [DOI] [PubMed] [Google Scholar]
  12. Hightower L. E., Morrison T. G., Bratt M. A. Relationships among the polypeptides of Newcastle disease virus. J Virol. 1975 Dec;16(6):1599–1607. doi: 10.1128/jvi.16.6.1599-1607.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lamb R. A., Choppin P. W. Determination by peptide mapping of the unique polypeptides in Sendai virions and infected cells. Virology. 1978 Feb;84(2):469–478. doi: 10.1016/0042-6822(78)90263-5. [DOI] [PubMed] [Google Scholar]
  14. Lamb R. A., Etkind P. R., Choppin P. W. Evidence for a ninth influenza viral polypeptide. Virology. 1978 Nov;91(1):60–78. doi: 10.1016/0042-6822(78)90355-0. [DOI] [PubMed] [Google Scholar]
  15. Markwell M. A., Fox C. F. Protein-protein interactions within paramyxoviruses identified by native disulfide bonding or reversible chemical cross-linking. J Virol. 1980 Jan;33(1):152–166. doi: 10.1128/jvi.33.1.152-166.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. PARROTT R. H., VARGOSKO A. J., KIMHW, BELL J. A., CHANOCK R. M. Acute respiratory diseases of viral etiology. III. parainfluenza. Myxoviruses. Am J Public Health Nations Health. 1962 Jun;52:907–917. doi: 10.2105/ajph.52.6.907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Paterson R. G., Harris T. J., Lamb R. A. Fusion protein of the paramyxovirus simian virus 5: nucleotide sequence of mRNA predicts a highly hydrophobic glycoprotein. Proc Natl Acad Sci U S A. 1984 Nov;81(21):6706–6710. doi: 10.1073/pnas.81.21.6706. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Robbins S. J., Bussell R. H., Rapp F. Isolation and partial characterization of two forms of cytoplasmic nucleocapsids from measles virus-infected cells. J Gen Virol. 1980 Apr;47(2):301–310. doi: 10.1099/0022-1317-47-2-301. [DOI] [PubMed] [Google Scholar]
  19. Scheid A., Caliguiri L. A., Compans R. W., Choppin P. W. Isolation of paramyxovirus glycoproteins. Association of both hemagglutinating and neuraminidase activities with the larger SV5 glycoprotein. Virology. 1972 Dec;50(3):640–652. doi: 10.1016/0042-6822(72)90418-7. [DOI] [PubMed] [Google Scholar]
  20. Scheid A., Choppin P. W. Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity of proteolytic cleavage of an inactive precursor protein of Sendai virus. Virology. 1974 Feb;57(2):475–490. doi: 10.1016/0042-6822(74)90187-1. [DOI] [PubMed] [Google Scholar]
  21. Scheid A., Choppin P. W. Protease activation mutants of sendai virus. Activation of biological properties by specific proteases. Virology. 1976 Jan;69(1):265–277. doi: 10.1016/0042-6822(76)90213-0. [DOI] [PubMed] [Google Scholar]
  22. Scheid A., Choppin P. W. Two disulfide-linked polypeptide chains constitute the active F protein of paramyxoviruses. Virology. 1977 Jul 1;80(1):54–66. doi: 10.1016/0042-6822(77)90380-4. [DOI] [PubMed] [Google Scholar]
  23. Shibuta H., Kanda T., Adachi A., Yogo Y. Characterization of bovine parainfluenza virus type 3. Microbiol Immunol. 1979;23(7):617–628. doi: 10.1111/j.1348-0421.1979.tb00502.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Shioda T., Hidaka Y., Kanda T., Shibuta H., Nomoto A., Iwasaki K. Sequence of 3,687 nucleotides from the 3' end of Sendai virus genome RNA and the predicted amino acid sequences of viral NP, P and C proteins. Nucleic Acids Res. 1983 Nov 11;11(21):7317–7330. doi: 10.1093/nar/11.21.7317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Storey D. G., Dimock K., Kang C. Y. Structural characterization of virion proteins and genomic RNA of human parainfluenza virus 3. J Virol. 1984 Dec;52(3):761–766. doi: 10.1128/jvi.52.3.761-766.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sánchez A., Banerjee A. K. Studies on human parainfluenza virus 3: characterization of the structural proteins and in vitro synthesized proteins coded by mRNAs isolated from infected cells. Virology. 1985 May;143(1):45–54. doi: 10.1016/0042-6822(85)90095-9. [DOI] [PubMed] [Google Scholar]
  27. Wechsler S. L., Lambert D. M., Galinski M. S., Pons M. W. Intracellular synthesis of human parainfluenza type 3 virus-specified polypeptides. J Virol. 1985 Jun;54(3):661–664. doi: 10.1128/jvi.54.3.661-664.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

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