Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1968 Dec;96(6):1940–1946. doi: 10.1128/jb.96.6.1940-1946.1968

Pyrogenic Responses to Staphylococcal Enterotoxins A and B in Cats1

Wesley G Clark a, John S Page a
PMCID: PMC252535  PMID: 5724965

Abstract

Pyrogenic responses, ranging up to 4.8 F, were induced in cats by oral administration of highly purified staphylococcal enterotoxin B in doses from 10 to 100 μg/kg. Fever was a more sensitive indicator of intoxication than was emesis. Highly purified preparations of enterotoxin A, whether administered intravenously (0.01 to 1.0 μg/kg), orally (10 to 25 μg/kg), or into the cerebral ventricles (0.005 to 0.020 μg in 0.20 ml), were also pyrogenic in cats. Tolerance to the pyrogenic activity was produced by repeated intravenous injection of a given dose of enterotoxin A but not by repeated intracerebroventricular injection. Enterotoxin A was more potent than enterotoxin B after intravenous injection in causing both fever and emesis. Cross-tolerance could not be demonstrated between enterotoxin A and enterotoxin B or Salmonella typhosa endotoxin. This lack of cross-tolerance plus the inability of large oral doses (100 to 4,700 μg/kg) of endotoxin to cause fever or emesis indicate that the reported responses were attributable to the specific toxins administered and not to contamination by other pyrogens.

Full text

PDF
1940

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERGDOLL M. S., SUGIYAMA H., DACK G. M. Staphylococcal enterotoxin. I. Purification. Arch Biochem Biophys. 1959 Nov;85:62–69. doi: 10.1016/0003-9861(59)90447-3. [DOI] [PubMed] [Google Scholar]
  2. CASMAN E. P. Further serological studies of staphylococcal enterotoxin. J Bacteriol. 1960 Jun;79:849–856. doi: 10.1128/jb.79.6.849-856.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CLARK W. G., BORISON H. L. PYROGENIC EFFECT OF PURIFIED STAPHYLOCOCCAL ENTEROTOXIN. J Pharmacol Exp Ther. 1963 Nov;142:237–241. [PubMed] [Google Scholar]
  4. Casman E. P., Bennett R. W., Dorsey A. E., Issa J. A. Identification of a fourth staphylococcal enterotoxin, enterotoxin D. J Bacteriol. 1967 Dec;94(6):1875–1882. doi: 10.1128/jb.94.6.1875-1882.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Casman E. P. Staphylococcal enterotoxin. Ann N Y Acad Sci. 1965 Jul 23;128(1):124–131. doi: 10.1111/j.1749-6632.1965.tb11634.x. [DOI] [PubMed] [Google Scholar]
  6. Chu F. S., Thadhani K., Schantz E. J., Bergdoll M. S. Purification and characterization of staphylococcal enterotoxin A. Biochemistry. 1966 Oct;5(10):3281–3289. doi: 10.1021/bi00874a030. [DOI] [PubMed] [Google Scholar]
  7. Crawley G. J., Gray I., Leblang W. A., Blanchard J. W. Blood binding, distribution and excretion of staphylococcal enterotoxin in monkeys. J Infect Dis. 1966 Feb;116(1):48–56. doi: 10.1093/infdis/116.1.48. [DOI] [PubMed] [Google Scholar]
  8. DACK G. M. STAPHYLOCOCCUS ENTEROTOXIN: A REVIEW. Jpn J Med Sci Biol. 1963 Feb;16:1–12. doi: 10.7883/yoken1952.16.1. [DOI] [PubMed] [Google Scholar]
  9. Greisman S. E., Young E. J., Woodward W. E. Mechanisms of endotoxin tolerance. IV. Specificity of the pyrogenic refractory state during continuous intravenous infusions of endotoxin. J Exp Med. 1966 Nov 1;124(5):983–1000. doi: 10.1084/jem.124.5.983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hodoval L. F., Morris E. L., Crawley G. J., Beisel W. R. Pathogenesis of lethal shock after intravenous staphylococcal enterotoxin B in monkeys. Appl Microbiol. 1968 Feb;16(2):187–192. doi: 10.21236/ad0666852. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lamanna C., Carr C. J. The botulinal, tetanal, and enterostaphylococcal toxins: a review. Clin Pharmacol Ther. 1967 Mar-Apr;8(2):286–332. doi: 10.1002/cpt196782286. [DOI] [PubMed] [Google Scholar]
  12. Martin W. J., Marcus S. Relation of pyrogenic and emetic properties of enterobacteriaceal endotoxin and of staphylococcal enterotoxin. J Bacteriol. 1964 May;87(5):1019–1026. doi: 10.1128/jb.87.5.1019-1026.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McCarthy L. E., Borison H. L. Volumetric compartmentalization of the cranial cerebrospinal fluid system determined radiographically in the cat. Anat Rec. 1966 Jul;155(3):305–313. doi: 10.1002/ar.1091550304. [DOI] [PubMed] [Google Scholar]
  14. Prohaska J. Development and fate of experimentally induced enteritis. Gastroenterology. 1966 Nov;51(5):913–925. [PubMed] [Google Scholar]
  15. Schantz E. J., Roessler W. G., Wagman J., Spero L., Dunnery D. A., Bergdoll M. S. Purification of staphylococcal enterotoxin B. Biochemistry. 1965 Jun;4(6):1011–1016. doi: 10.1021/bi00882a005. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES