Abstract
Preferential binding of guanine-rich ribopolymers to one of the complementary strands of denatured deoxyribonucleic acid (DNA) of Diplococcus pneumoniae permitted the fractionation of the complements in CsCl density gradients. Phenotypic expression of the newly acquired genes for four drug resistances was more rapid in cells transformed by the heavy fractions than in those transformed either by light fractions or by unfractionated DNA. The efficiencies of transformation with the two complements were nearly equal for the four markers tested. Both efficiency and expression results were the same whether we assayed the residual activity or the activity obtained by annealing the fractions with excess recipient DNA.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Alberts B. M., Doty P. Characterization of a naturally occurring, cross-linked fraction of DNA. 1. Nature of the cross-linkage. J Mol Biol. 1968 Mar 14;32(2):379–403. doi: 10.1016/0022-2836(68)90017-x. [DOI] [PubMed] [Google Scholar]
- Chilton M. D. Transforming Activity in Both Complementary Strands of Bacillus subtilis DNA. Science. 1967 Aug 18;157(3790):817–819. doi: 10.1126/science.157.3790.817. [DOI] [PubMed] [Google Scholar]
- GUILD W. R. Evidence for intramolecular heterogeneity in pneumococcal DNA. J Mol Biol. 1963 Mar;6:214–229. doi: 10.1016/s0022-2836(63)80071-6. [DOI] [PubMed] [Google Scholar]
- GUILD W. R., ROBINSON M. Evidence for message reading from a unique strand of pneumococcal DNA. Proc Natl Acad Sci U S A. 1963 Jul;50:106–112. doi: 10.1073/pnas.50.1.106. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gabor M., Hotchkiss R. D. Manifestation of linear organization in molecules of pneumococcal transforming DNA. Proc Natl Acad Sci U S A. 1966 Nov;56(5):1441–1448. doi: 10.1073/pnas.56.5.1441. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gurney T., Jr, Fox M. S. Physical and genetic hybrids formed in bacterial transformation. J Mol Biol. 1968 Feb 28;32(1):83–100. doi: 10.1016/0022-2836(68)90147-2. [DOI] [PubMed] [Google Scholar]
- Mulder C., Doty P. Residual activity of denatured transforming DNA of Haemophilus influenzae: a natrually occurring cross-linked DNA. J Mol Biol. 1968 Mar 14;32(2):423–435. doi: 10.1016/0022-2836(68)90019-3. [DOI] [PubMed] [Google Scholar]
- OPARA-KUBINSKA Z., KUBINSKI H., SZYBALSKI W. INTERACTION BETWEEN DENATURED DNA, POLYRIBONUCLEOTIDES, AND RIBOSOMAL RNA: ATTEMPTS AT PREPARATIVE SEPARATION OF THE COMPLEMENTARY DNA STRANDS. Proc Natl Acad Sci U S A. 1964 Oct;52:923–930. doi: 10.1073/pnas.52.4.923. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]