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. 1968 Dec;96(6):2004–2011. doi: 10.1128/jb.96.6.2004-2011.1968

Biosynthesis of Arginine in L Cells Infected with Chlamydiae

Mary W Treuhaft 1, James W Moulder 1
PMCID: PMC252551  PMID: 5724968

Abstract

Three members of the genus Chlamydia were examined for their ability to synthesize arginine, an ability their L cell (mouse fibroblasts) hosts lacked. C. psittaci (strain 6BC) multiplied in arginine-free medium 199 without significant decrease in titer and incroporated 14C-glutamate and 14C-ornithine into the arginine fraction of its protein. In arginine-free media, C. trachomatis (strain mouse pneumonitis) and C. psittaci (strain meningopneumonitis) grew to only 1 to 10% of the titer obtained in arginine-containing media. The decreased ability of these two strains to multiply in arginine-free media was paralleled by a decreased ability of infected host cells to incorporate 14C-glutamate into protein arginine. These results suggest that chlamydiae either synthesize arginine themselves, or, in some unknown manner, cause their host cells to do so.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander J. J. Separation of protein synthesis in meningopneumonitisgent from that in L cells by differential susceptibility to cycloheximide. J Bacteriol. 1968 Feb;95(2):327–332. doi: 10.1128/jb.95.2.327-332.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BADER J. P., MORGAN H. R. Latent viral infection of cells in tissue culture. VI. Role of amino acids, glutamine, and glucose in psittacosis virus propagation in L cells. J Exp Med. 1958 Nov 1;108(5):617–630. doi: 10.1084/jem.108.5.617. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  4. EAGLE H., PIEZ K. A., LEVY M. The intracellular amino acid concentrations required for protein synthesis in cultured human cells. J Biol Chem. 1961 Jul;236:2039–2042. [PubMed] [Google Scholar]
  5. ENNIS H. L., LUBIN M. CYCLOHEXIMIDE: ASPECTS OF INHIBITION OF PROTEIN SYNTHESIS IN MAMMALIAN CELLS. Science. 1964 Dec 11;146(3650):1474–1476. doi: 10.1126/science.146.3650.1474. [DOI] [PubMed] [Google Scholar]
  6. HESS J., KITO E., MARTIN R. P., VAN PILSUM J. F. Determination of creatine, creatinine, arginine, guanidinoacetic acid, guanidine, and methylguanidine in biological fluids. J Biol Chem. 1956 Sep;222(1):225–235. [PubMed] [Google Scholar]
  7. MOULDER J. W., GRISSO D. L., CHO G. J. ENDOGENOUS METABOLISM OF PROTEIN AND RIBONUCLEIC ACID IN A MEMBER OF THE PSITTACOSIS GROUP. J Infect Dis. 1965 Jun;115:254–262. doi: 10.1093/infdis/115.3.254. [DOI] [PubMed] [Google Scholar]
  8. MOULDER J. W., NOVOSEL D. L., TRIBBY I. C. DIAMINOPIMELIC ACID DECARBOXYLASE OF THE AGENT OF MENINGOPNEUMONITIS. J Bacteriol. 1963 Mar;85:701–706. doi: 10.1128/jb.85.3.701-706.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Manire G. P., Tamura A. Preparation and chemical composition of the cell walls of mature infectious dense forms of meningopneumonitis organisms. J Bacteriol. 1967 Oct;94(4):1178–1183. doi: 10.1128/jb.94.4.1178-1183.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McLIMANS W. F., DAVIS E. V., GLOVER F. L., RAKE G. W. The submerged culture of mammalian cells; the spinner culture. J Immunol. 1957 Nov;79(5):428–433. [PubMed] [Google Scholar]
  11. Moore D. E., Moulder J. W. Autoradiographic study of deoxyribonucleic acid synthesis in L cells infected with the agent of meningopneumonitis. J Bacteriol. 1966 Oct;92(4):1128–1132. doi: 10.1128/jb.92.4.1128-1132.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Moulder J. W. The relation of the psittacosis group (Chlamydiae) to bacteria and viruses. Annu Rev Microbiol. 1966;20:107–130. doi: 10.1146/annurev.mi.20.100166.000543. [DOI] [PubMed] [Google Scholar]
  13. OSSOWSKI L., BECKER Y., BERNKOPF H. AMINO ACID REQUIREMENTS OF TRACHOMA STRAINS AND OTHER AGENTS OF THE PLT GROUP IN CELL CULTURE. Isr J Med Sci. 1965 Mar;1:186–193. [PubMed] [Google Scholar]
  14. SCHIMKE R. T. ENZYMES OF ARGININE METABOLISM IN MAMMALIAN CELL CULTURE. I. REPRESSION OF ARGININOSUCCINATE SYNTHETASE AND ARGININOSUCCINASE. J Biol Chem. 1964 Jan;239:136–145. [PubMed] [Google Scholar]
  15. Schechter E. M. Synthesis of nucleic acid and protein in L cells infected with the agent of meningopneumonitis. J Bacteriol. 1966 May;91(5):2069–2080. doi: 10.1128/jb.91.5.2069-2080.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tribby I. I., Moulder J. W. Availability of bases and nucleosides as precursors of nucleic acids in L cells and in the agent of meningopneumonitis. J Bacteriol. 1966 Jun;91(6):2362–2367. doi: 10.1128/jb.91.6.2362-2367.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]

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