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. 1968 Dec;96(6):2025–2034. doi: 10.1128/jb.96.6.2025-2034.1968

Inhibition of Membrane Transport in Streptococcus faecalis by Uncouplers of Oxidative Phosphorylation and Its Relationship to Proton Conduction

F M Harold 1, J R Baarda 2
PMCID: PMC252554  PMID: 4177737

Abstract

We studied the effect of compounds that uncouple oxidative phosphorylation on membrane function in Streptoccocus faecalis, an organism which relies upon glycolysis for the generation of metabolic energy. At low concentrations (ranging from 10−7 to 10−4m), tetrachlorosalicylanilide, tetramethyldipicrylamine, carbonylcyanide m-chlorophenylhydrazone, pentachlorophenol, and dicoumarol strongly inhibited energy-dependent transport of rubidium, phosphate, and certain amino acids. However, these compounds had little effect on the generation of adenosine triphosphate via glycolysis or on its utilization for the synthesis of macromolecules. They also did not seriously inhibit uptake of those monosaccharides and amino acids which do not require concurrent metabolism. It is proposed that the uncouplers interfere with the utilization of metabolic energy for membrane transport. The uncouplers accelerated the translocation of protons across the cytoplasmic membrane. It appears that a proton-impermeable membrane is required for transport, perhaps, because a proton gradient is involved in the coupling of metabolic energy to the translocation of substrates across the membrane.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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