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. 1985 Nov;56(2):625–627. doi: 10.1128/jvi.56.2.625-627.1985

Amplification of a bovine papillomavirus-simian virus 40 chimera.

R B DuBridge, M Lusky, M R Botchan, M P Calos
PMCID: PMC252622  PMID: 2997482

Abstract

A chimeric plasmid, pBOP, containing bovine papillomavirus (BPV) and the origin of replication from simian virus 40 (SV40) was constructed. The plasmid was established in mouse cells, where it was maintained stably as an autonomous BPV replicon. Lines carrying pBOP were fused to cells of COS-7, a simian line producing SV40 T antigen. Replication dependent on the SV40 origin and having the kinetics and approximate amplitude of an SV40 infection ensued. SV40 replication is therefore dominant over BPV replication, and the SV40 origin can conveniently be used to amplify lower-copy-number plasmids in mammalian cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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