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. 1985 Nov;56(2):628–632. doi: 10.1128/jvi.56.2.628-632.1985

In vitro synthesis of an infectious RNA from cDNA clones of human rhinovirus type 14.

S Mizutani, R J Colonno
PMCID: PMC252623  PMID: 2997483

Abstract

Development of a novel infectious cDNA assay is described for human rhinovirus type 14. A full-length cDNA clone of the human rhinovirus type 14 genome RNA was assembled and transcribed in vitro by using the SP6 transcription system. Transfection of HeLa cells with the nascent RNA resulted in the production of rhinovirus indistinguishable from the parental virus by both immunological and polyacrylamide gel analysis.

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Selected References

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