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. Author manuscript; available in PMC: 2009 Jul 21.
Published in final edited form as: Carbohydr Res. 2008 May 8;343(10-11):1594–1604. doi: 10.1016/j.carres.2008.05.003

Varied Presentation of the Thomsen-Friedenreich Disaccharide Tumor-Associated Carbohydrate Antigen on Gold Nanoparticles

Andreas Sundgren 1, Joseph J Barchi Jr 1,*
PMCID: PMC2526251  NIHMSID: NIHMS57844  PMID: 18502409

Abstract

Three-dimensional self-assembled monolayers of gold coated with the Thomsen-Friedenreich antigen (TFag) disaccharide (β-Galp-(1→3)-GalpNAc) in a variety of presentations have been prepared and characterized. Anomalies in the size distribution of our originally synthesized TFag-bearing nanoparticles as shown in dynamic light scattering experiments prompted us to explore the effect of antigen density on the uniformity of the particles. Gold nanoparticles containing a range of densities “diluted” with copies of the PEG-thiol spacer unit showed that lower antigen density affords more uniform particles. We also wanted to study the constitution of the actual antigen by synthesizing nanoparticles not only with the linker-extended disaccharide, but within the context of the surrounding peptide sequence where it may be presented in vivo. The synthesis of thiol-containing TFag-containing glycopeptides from a mucin peptide repeating unit were prepared, assembled into gold nanoparticles and their physical properties evaluated. These novel multivalent tools should prove extremely useful in exploring the binding properties and immune response to this important carbohydrate antigen.

Keywords: Thomsen-Friedenreich antigen, Gold nanoparticles, Glycopeptides, Immunogen

1. Introduction

Spurred by recent advances in technology development, there has been a surge in the design, development and the use of nanoscale platforms for many areas of medical research. Various materials (such as gold1, iron oxide2 and semiconductor materials3) can be employed to form self-assembled structures that can be coated with organic agents or biomacromolecules. These novel nanoparticles (NP’s) have intriguing physical properties that may be related to their core materials and nanometer size ranges, as well as the chemistry of the coating itself. The field of carbohydrate chemistry has benefited from this technology, as many platforms allow the presentation of multiple copies of a particular molecule (or many different molecules) on their surface, yielding multivalent constructions that can be used to study and/or interfere with carbohydrate-protein interactions. Hence, “glyconanotechnology”48 is a burgeoning field and there are now a host of published examples of glyconanoparticles (GNP’s) that have shown strong potential to act as extremely useful tools in glycan science. One of the most widely used platforms that have often been “sugar-coated” is that which employs spherical self assembled monolayers of gold. These gold NP’s have a strong affinity for organic thiols and their synthesis has been refined to where highly stable and relatively monodispersed particles can be rapidly produced through simple reduction of gold salts in the presence of a molecule of choice tethered appropriately to a terminal mercapto group.9 Thus, mono- and oligosaccharides have been displayed on the surface of gold NP’s and shown to have provocative characteristics compared with their monovalent counterparts.10 In addition, carbohydrates have been attached on quantum dots1115 for cell imaging applications and on magnetic NP’s16 for differential separation of bacterial strains.

Our interest in this area is targeted to the synthesis of GNP’s with tumor associated carbohydrate antigens1719 (TACA’s) on their surface. TACA’s are aberrantly expressed carbohydrate units found on the cell surface of tumors that derive from the differences in the way glycans are processed in malignant cells compared to normal phenotypes. Our rationale for the design of these particles was twofold: 1) They have the potential to be used as a novel vaccine platform20 to elicit an immune response in vivo, or, 2) They could be useful inhibitors of well-known protein carbohydrate interactions in which specific TACA’s are involved. For these reasons we concentrated on the Thomsen-Friedenreich disaccharide (TFag)21,22, a human TACA present primarily in carcinomas but rarely expressed in normal tissues.23 This antigen has been the subject of many studies attempting to design immunogens or vaccine platforms targeted to this TACA.2426 In addition, tumors displaying the TFag metastasize through a specific interaction with endothelial cell-derived galectin-3 (Gal-3).27,28 Both adhesion to Gal-3 and cell growth are inhibited by TFag function-blocking or anti galectin-3 antibodies. Another report corroborated the importance of the TFag–Gal-3 interaction by showing that Gal-3 interacts with the cancer-associated mucin MUC1 via TFag.29 This interaction caused a polarization of MUC1 on the cell surface revealing epithelial adhesion molecules that are otherwise concealed by MUC1, thus promoting cancer cell adhesion to the endothelium. The fact that TFag is covalently attached to the peptide backbone of MUC1, suggests that the surrounding peptide sequence is part of the antigen that is recognized by the immune system, and hence synthetic glycopeptides may be better immunogens than the “naked” dissacharide.

We mentioned in our earlier report30 that TFag-coated GNP’s could inhibit lung metastasis in the murine 4T1 breast cancer model. The details of this work were never published since we unfortunately were not able to reproduce the original result in several subsequent studies using different nanoparticle concentrations and control experiments with particles coated simply with hydroxyl-terminated linker groups (vide infra). Although we showed that TFag-coated GNP’s agglutinated TFag-specific antibodies, TFag-coated GNP’s were unable to inhibit TFag-specific antibody binding to immobilized BSA-conjugated TFag (Barchi, Rittenhouse-Olson, Heimburg, Sundgren, unpublished results). A closer examination revealed that the TFag-coated GNP’s were polydispersed (non-uniform size distribution) in solution by dynamic light scattering (DLS) experiments (vide infra). We reasoned that this may be the source of the problems with our in vivo experiments and prompted us to refine the synthesis of the NP’s to increase their mondispersity and attempt to determine the causes of the aforementioned non-uniformity. In addition, we synthesized the antigen in different forms including within a tumor-associated glycopeptide construction from a mucin protein (MUC4).

2. Results and Discussion

Our synthesis of TFag-coated GNP’s employed the reduction of gold salts by NaBH4 in the presence of specific carbohydrate-linked thiols.31,32 This method has been utilized in many GNP reports cited above and the results, in general, have been quite successful. In our previous work, we synthesized TFag-coated GNP’s with approximately 90 sugar units on the gold surface 1 along with the unnatural β-analogue 2 to compare the quality and properties of these particles with the α-linked GNP’s. For our in vivo studies, we had prepared the control particles 3, which displayed simply the linker molecule where the TFag disaccharide is essentially substituted with a simple hydroxyl group (Figure 1).

Figure 1.

Figure 1

TFag (1), β-TFag (2) and linker “control” (3) GNP’s.

The synthesis of the pentenyl-hexaPEG linker and TFag-linked thiol was as described previously.30 The GNP’s and linker-coated particles were purified by ultrafiltration and analyzed by transmission electron microscopy (TEM), NMR spectroscopy, elemental analysis and dynamic light scattering (DLS). The core diameter of the GNP’s 1 and 2 was in the 5–7 nm range by TEM measurements, whereas NP’s 3 with simply the linker were slightly larger and somewhat more polydispersed but in solution looked superior to the disaccharide-bearing particles (Figure 2). In comparing the GNP’s 1–3 by DLS, the α-linked TFag particles 1 were the least uniform and their hydrodynamic diameter (HDD) was also twice as large as β-linked TFag GNP’s when volumes are considered (Figure 2).

Figure 2.

Figure 2

DLS data for compounds 1–3. Top trace is intensity vs. size while the bottom traces are volumes vs. size. The lower row shows TEM data for each NP with size histograms as an inset.

Initially, our hypothesis was that the anomeric stereochemistry of the sugar could cause problems in the self-assembly process of the nanoparticles. Very few α-linked sugars have been coated on gold nanoparticles.33 To our knowledge, only one group has synthesized α-linked disaccharides on gold and they showed that different aggregation properties were observed when comparing α and β-linked disaccharide-coated particles.34,35 If the directionality of the sugar is angled differently in the α- and β-linked GNP’s and this is the source of aberrations in the self-assembly process, reducing the carbohydrate density on the surface may facilitate the production of more uniform particles yielding a tighter distribution of sizes. Scheme 1 outlines the synthesis of GNP’s with various ratios of carbohydrate ligand to underivatized linker thiol used in the reaction mixture. As the density of the carbohydrate is lowered on the particle surface, the size distribution also improves (see Supporting Information for DLS data on particles 6–9). In addition, the optimum uniformity is observed for the particles prepared where a 5:1 linker-to-sugar ratio is used (compound 10). This method is being used by us for the comparison of various particles with different passivating agents and surface coatings. For example, we have prepared simple β-galactose-coated GNP’s from compounds where the linker-to-sugar ratio was either 0:1, 1:1 or 5:1. Microscopy and sizing data also showed that the 5:1 linker-to-sugar particles have the most uniform size by both DLS and TEM analysis and the ability for these to inhibit or promote HIV fusion to mammalian cells changes with carbohydrate density (unpublished results).

Scheme 1.

Scheme 1

i) HAuCl4, NaBH4, H2O, 0 °C; DLS data for compound 10 is shown in the lower right with intensity vs. size shown in the top trace and volume vs. size in the bottom trace.

The scientific literature is replete with reviews about TACA’s along with suggestions on the exact constitution of the actual antigen “structures”. Many (including us) believe the recognition element of a specific glycoprotein-based TACA includes either the amino acid or local peptide sequence to which it is attached in is natural cellular context. In addition, this “natural” antigen should elicit a more powerful immune response in synthetic vaccine preparations since it should more closely resemble the conformation displayed in vivo. Recent work has shown that nanoparticle platforms have the potential to act as single-36,37 or multi-component20 vaccine scaffolds. We thus extended the antigen “structure” to the TFag coupled to specific mucin peptides. Mucins are very large proteins that act as a lubricant and can protect the cell surface from outside insult. The long protein backbone is comprised of many repeating motifs of between ~16–22 amino acids that contain several serine and threonine residues of which the majority are glycosylated.38 This serves to “extend” the protein backbone straight out from the cell surface, and the inter-digitation of the mucin molecules forms a protective barrier as part of the glycocalyx. Several mucins are overexpressed in various cancers; structures related to both the carbohydrate and the peptide backbone of mucins have been employed in antitumor vaccines strategies. We chose the 16-amino acid repeating unit of MUC4 (Figure 3), the primary mucin found on adenocarcinomas of the pancreas,3942 for our studies to prepare glycopeptide-coated nanoparticles (GPNP’s). The Kunz group had previously synthesized several glycopeptides of MUC4 by solid phase peptide synthesis for use in immunological studies. We followed similar procedures to make a repertoire of glycopeptides for assembly on gold particles.

Figure 3.

Figure 3

Structure of MUC416 peptide and two glycopeptides with TFag at positions Thr6 and Thr10. The linker R3 was used to functionalize the N-terminus of the glycopeptide for GPNP synthesis.

We have synthesized glycopeptides with the TFag at two separate positions and developed appropriate linker chemistry for the synthesis of GPNP’s (Scheme 2).

Scheme 2.

Scheme 2

i) DIPCDI, HOBt, DMF, dichloromethane (CH2Cl2), 86%; ii) Thiolacetic acid, AIBN, 84%; iii) Piperidine, CH2Cl2; iv) AcS(CH2)6COOH (15), DIPCDI, HOBt, DMF, CH2Cl2, 86% in two steps; v) TFA, 72%; vi) 2-Amino ethanol, DIPCDI, HOBt, DMF, CH2Cl2; vii) Hydrazine hydrate, EtOH; viii) Dithiothreitol, water, 85% in three steps; ix) 17, DIPCDI, HOBt, DMF; x) Hydrazine hydrate, EtOH; xi) 95% TFA, 2.5% 1,2-ethanedithiol.

SPPS of the peptides proceeded smoothly for the majority of the coupling reactions. We employed Fmoc chemistry using a mixture of HOBt and HBTU for activation. Synthesis was performed mostly in the automated mode on a peptide synthesizer. The glycoamino acid, however, was coupled manually and depending on the position of the sugar unit in the peptide sequence, specific conditions were needed to affect efficient coupling. The two glycopeptides we report on here had the TFag at the 6th and 10th threonine residues (Figure 3). We prepared the appropriately functionalized control peptide (without a covalently attached carbohydrate) attached to the linker unit for self assembly onto gold particles. The linker strategy is one that deserves mention, since this can be a critical component in the production of NP’s. The glycopeptide linker described here incorporated a PEG unit and a fatty portion which can potentially facilitate packing at the surface. Linkers consisting solely of PEG units were difficult to couple to the peptide and did not self assemble efficiently. An extender glycine amino acid was added to bolster the reactivity of the carboxylate toward coupling with the amino group of the peptide. The commercially available N-Fmoc-1-amino-3,6,9,12,15,18-hexaoxahenicosan-21-oic acid was reacted according to Scheme 2 to yield the linker 17 which was coupled to the peptide on resin. Deprotection of the acetate groups was also accomplished most efficiently while attached to the resin with hydrazine/ethanol. Coupling of the glycoamino acids was performed with HOBt–diisopropylcarbodiimide (DIPCDI), and although efficient, was slow and required extended reaction times and additional activating reagents to reach completion. Experimentation with microwave catalysis to improve the yield and increase the rate of these coupling steps is in progress. Scheme 3 outlines the method for the preparation of GPNP’s 20–22 from the compounds 12 and 13.

Scheme 3.

Scheme 3

i) HAuCl4, NaBH4, H2O, 0 °C.

In addition to preparing the peptide-bearing particles 19 and GPNP’s 20 and 21, we prepared the “diluted” particles with a 1:5 ratio of glycopeptide to linker (22) along with another “control” particle containing the linker alone (23). It was necessary to modify the linker 17 used in the synthesis of 23 by extension with an ethanolamine tail to produce 18 for the preparation of particles that expose simple hydroxyl groups on their surface similar to our approach for linker-coated NP’s 3. The rationale for producing two “control” particles (19 and 23) is to be able to dissect the effects of the sugars, the peptide backbone and the linker on the recognition of the GPNP’s. Physical characterization of particles 19–23 followed similarly as for the GNP’s above. An estimate as to the number of copies of glycopeptide exposed on the GPNP’s was made from elemental analysis and core diameters that estimate the number of gold atoms in the particle composition.43 The glycopeptides 20 and 21 contained ca. 180 copies of ligand while for the peptide alone (19) there are an estimated 220 copies. The peptide and glycopeptide-conjugated gold particles all looked very uniform by TEM analysis (Figure 4). As has been shown in several past reports including from our laboratory, NMR of the GNP’s or GPNP’s in water show nearly all signals that are evident in the monomeric ligands, but broadened by the high molecular weight and changes to relaxation times in the NP’s (data not shown). A surprising discovery during this work was that, even with identical synthetic procedures used throughout to prepare NP’s, uniformity was dictated by the surface chemistry and could not be predicted a priori. Hence, the disaccharide-coated NP’s that were prepared typically showed polydispersity unless “diluted” with interstitial spacer PEG linkers to reduce the saccharide density on the surface of the particle. Glycopeptides were consistently more uniform by microscopic analysis, but in all particles that we have made to date, DLS analysis shows intensity bands in the range of 50–150 nm. These usually disappear on conversion of these intensity maps to volumes or when the particles are first filtered through membranes that exclude all material above 0.1 um, indicating that although there may be particles that are much larger in hydrodynamic diameter (HDD) and scatter light efficiently, their actual number is quite low and the bulk of the particles are in the 7–11 nm HDD range. We are now in the process of testing these novel particles in several bioassays and the results will be reported in due course.

Figure 4.

Figure 4

TEM data for GPNP, peptide- and linker-coated NP’s 19–23.

3. Conclusions and Summary

Gold nanoparticles bearing the Thomsen Friedenreich antigen at different densities and in different contexts have been prepared and characterized. Sizing data showed that certain sugar presentations result in the assembly of particles that may have reasonable uniformity in their gold core diameters by TEM analysis, but they can be polydispersed and “non-uniform” in solution. Glycopeptide-coated nanoparticles result in more uniform particles as seen by TEM analysis, however, GNP’s and GPNP’s contain some larger size elements that could be a consequence of either aberrations in the self assembly process or aggregation events. An important conclusion gleaned from this work is that nanoconstructions with various surface chemistries may display very different behavior when comparing microscopy to sizing measurements in solution. By comparing measurements performed in various milieus (water, 10 mM NaCl, PBS, data not shown), it is evident that each particle has their own individual properties when exposed to different solutions. This could have important consequences when studying these particles in a cellular or in vivo context. Subsequent reports will outline in detail the “idiosyncrasies” of each GNP(GPNP) and how these relate to their biological activity and therapeutic potential.

3. Experimental

Flash column chromatography (FCC) was performed using RediSep® silica columns on a CombiFlash® Companion® employing solvent polarity gradient (hexane → ethyl acetate). Reversed phase chromatography was performed on 900 mg Alltech® Maxi-Clean™ C18 Cartridges employing solvent polarity gradient (water → methanol) unless otherwise noted. Chemicals were purchased from Aldrich-Sigma (Milwaukee, WI) and used without further purification. NMR spectra were recorded on a Varian Inova 400 instrument with residual CHCl3 (7.26 ppm) as the internal standard at frequencies of 399.74 MHz for 1H and 100.51 MHz for 13C. Assignments were based on gCOSY, TOCSY, ROESY, and 13C/DEPT experiments. 1H NMR data are tabulated in the order of multiplicity (s, singlet; d, doublet; dd, doublet of doublets; dt, double of triplets; t, triplet; q, quartet; m, multiplet; brs, broad signal), number of protons, and coupling constant(s) in Hertz. Specific optical rotations were determined using JASCO-P1010 polarimeter in 0.5 dm cuvette at 589 nm in chloroform. Five consecutive measurements were taken and the average value is reported. High resolution mass spectra were performed by Mass Spectrometry Facility at University of California, Riverside. Elemental analyses were performed by Atlantic Microlab, Inc., Norcross, GA and Galbraith Laboratories Inc., Knoxville, TN. Transmission electron micrographs were performed on a Hitachi H-7000 microscope equipped with a Gatan digital camera operating at 75 kV. A Malvern Zetasizer Nano ZS instrument (Southborough, MA) with back scattering detector was used for measuring the hydrodynamic size (diameter) in batch mode at 25 °C in a disposable low volume polystyrene microcuvette. Samples were measured at a concentration of 0.4 mg/mL or 0.2 mg/mL in both H2O and PBS. Samples were filtered through a 0.1 µm filter before a minimum of ten measurements were made. Hydrodynamic size is reported as the intensity-weighted average over all size populations (Z-avg), and the volume-weighted average over a particular range of size populations corresponding to the most prominent peak in the % volume distribution (Vol-Peak).

3.1 General procedure for synthesis of gold nanoparticles

Thiol (1 eq.) and 58 mM HAuCl4 (2.75 eq.) were added to water (10 mL/µmol thiol) and the obtained yellow solution was cooled to 0 °C. 0.1% NaBH4 in water (1 mL/µmol thiol) was then added over 10 min where upon the color changed to red or purple over the first minute of addition. This solution was then stirred at 0 °C for 2 h and at rt for another 16 h. The solution was concentrated to about 5 mL, purified utilizing a Centriplus 30K filter and the obtained pure solution of the particles was lyophilized to obtain a dark-purple solid.

3.2. t-Butyl N-Fmoc-1-amino-21-oxo-3,6,9,12,15,18-hexaoxa-22-azatetracosan-24-oate (14)

N-Fmoc-1-amino-3,6,9,12,15,18-hexaoxahenicosan-21-oic acid (Available from NeoMPS Inc., San Diego, CA) (520 mg, 904 µmol) was dissolved in CH2Cl2 (3 mL) and cooled to 0 °C. A solution of 0.5 M HOBt in DMF (2.36 mL, 1.18 mmol) and DIPCDI (180 µL, 1.18 mmol) were added, this was stirred for 30 min at 0 °C after which H-Gly-OtBu (152 mg, 904 µmol) was added. After stirring for 18 h, another portion of 0.5 M HOBt in DMF (4.70 mL, 2.35 mmol) and DIPCDI (362 µL, 2.35 mmol) were added and the reaction was stirred for another 48 h. The solvents were evaporated and the residue purified by column chromatography to give compound 14 (534 mg, 86%). 1H NMR (CDCl3): δ 1.46 (s, 9H, (CH3)3C)), 2.51 (t, 2H, J = 6.0 Hz), 3.40 (q, 2H, J = 5.6 Hz), 3.57 (t, 2H, J = 4.8 Hz), 3.62–3.64 (m, 20 H), 3.74 (t, 2H, J = 5.6 Hz), 3.92 (d, 2H, J = 5.6 Hz), 4.22 (t, 1H, J = 6.8 Hz), 4.40 (d, 2H, J = 6.8 Hz), 7.32 (dt, 2H, J = 1.2 Hz, J = 7.6 Hz, aromatic), 7.40 (dt, 2H, J = 0.8 Hz, J = 7.6 Hz), 7.61 (d, 2H, J = 7.6 Hz), 7.76 (d, 2H, J = 7.6 Hz); 13C NMR (CDCl3): δ 28.1, 36.7, 42.0, 47.3, 67.1, 70.30, 70.32, 70.5, 70.6, 81.6, 120.0, 125.1, 127.1, 127.7, 128.2, 129.1, 141.3, 144.0, 169.1, 171.7; HRMS [C36H52N2O11 + Na]+: calc. 711.347, found: 711.347.

3.3. 7-(thioacetyl)heptanoic acid (15)

Heptenoic acid (100 mg, 781 µmol) and AIBN (20 mg) were dissolved in MeOH (5 mL) containing thioacetic acid (200 µL). The flask was then irradiated with a 350 watt UV lamp for 6 h, concentrated and azeotroped with toluene. The residue was purified by column chromatography to yield compound 15 (133 mg, 84%). 1H NMR (CDCl3): δ 1.36–1.39 (m, 4H), 1.55–1.58 (m, 2H), 1.61–1.67 (m, 2H), 2.32 (s, 3H, CH3COS), 2.35 (t, 2H, J = 7.6 Hz), 2.86 (t, 2H, J = 7.2 Hz); 13C NMR (CDCl3): δ 24.6, 28.5, 28.6, 29.1, 29.4, 30.8, 34.1, 180.2, 196.2. HRMS [C9H16O3S + Na]+: calc. 227.072, found: 227.072.

3.4. tert-Butyl 3,25-dioxo-6,9,12,15,18,21-hexaoxa-31-acetylthio-24-azahentriacontan-1-oate (16)

Compound 14 (518 mg, 753 µmol) was dissolved in CH2Cl2 (8 mL) and piperidine (3 mL) and the solution was stirred for 3 h, concentrated and azeotroped with toluene. The residue was filtered through a short silica column to give the crude amine (386 mg), which was dissolved in CH2Cl2 (5 mL) together with 14 (154 mg, 753 µmol) followed by addition of 0.5 M HOBt in DMF (1.96 mL, 979 µmol) and DIPCDI (150 (150 µL, 979 µmol). The reaction was stirred for 18 h, concentrated and the residue purified by column chromatography to give 16 (421 mg, 86%). 1H NMR (CDCl3): δ 1.33–1.37 (m, 4H), 1.46 (s, 9H, (CH3)3C)), 1.55–1.61 (m, 2H), 1.62–1.67 (m, 2H), 2.18 (t, 2H, J = 7.6 Hz). 2.33 (s, 3H, CH3COS), 2.53 (t, 2H, J = 6.0 Hz), 2.86 (t, 2H, J = 7.2 Hz), 3.42–3.47 (m, 2H), 3.55 (t, 2H, J = 4.8 Hz), 3.62–3.64 (m, 22 H), 3.76 (t, 2H, J = 5.6 Hz), 3.93 (d, 2H, J = 5.6 Hz); Anal. Calcd for C30H56N2O11S: C 55.19; H 8.65; N 4.29, found: C 54.91; H 8.42; N 4.17.

3.5. 3,25-dioxo-6,9,12,15,18,21-hexaoxa-31-mercaptoheptanamido-24-azahentriacontan-1-oic acid (17)

Compound 16 (200 mg, 307 µmol) was dissolved in 90% TFA (5 mL) and the obtained solution was stirred for 40 min. The reaction was concentrated and the residue was purified by column chromatography to give 17 (132 mg, 72%). 1H NMR (CDCl3): δ 1.29–1.39 (m, 4H), 1.53–1.59 (m, 2H), 163 (p, 2H, J = 6.8 Hz), 2.21 (t, 2H, J = 7.6 Hz), 2.32 (s, 3H, CH3COS), 2.54 (t, 2H, J = 5.6 Hz), 2.85 (t, 2H, J = 7.6 Hz), 3.42–3.46 (m, 2H), 3.57 (t, 2H, J = 5.2 Hz), 3.64–3.67 (m, 20 H), 3.74 (t, 2H, J = 5.2 Hz), 4.07 (d, 2H, J = 5.2 Hz); 13C NMR (CDCl3): δ 25.6, 28.5, 28.8, 29.1, 29.4, 30.7, 36.5, 39.4, 41.6, 67.1, 70.0, 70.1, 70.27, 70.33, 70.4, 70.47, 70.50, 70.52, 70.6, 174.0, 196.2; HRMS [C26H48N2O11S + Na]+: calc. 619.288, found: 619.288; Anal. Calcd for C26H48N2O11S: C 52.33; H 8.11; N 4.69, found: C 52.02; H 7.89; N 4.71.

3.6. N-(2-(2-hydroxyethylamino)-2-oxoethyl)-1-(7-mercaptoheptanamido)-3,6,9,12,15,18-hexaoxahenicosan-21-amide (19)

Compound 17 (97 mg, 163 µmol) was dissolved in CH2Cl2 (1 mL), a solution of 0.5 M HOBt in DMF (976 µL, 488 µmol) and DIPCDI (61 mg, 488 µmol) were added and the obtained mixture was stirred for 10 min. 2-Amino-ethanol (30 µL, 488 µmol) was added and the reaction was stirred for 18 h. After concentration, the residue was filtered through a silica column (gradient from hexane to 10% MeOH in EtOAc) and the crude product was dissolved in 10% hydrazine hydrate in EtOH (3 mL). The solution was stirred for 16 h, concentrated and azeotroped with toluene. The residue was dissolved in water (1 mL) containing dithiothreitol (10 mg), stirred under argon for 14 h and then filtered through a reversed phase column to give 19 (91 mg, 85%). 1H NMR (CDCl3): δ 1.14 (p, 2H, J = 7.2 Hz), 1.23 (p, 2H, J = 7.2 Hz), 1.41–1.47 (m, 4H), 2.10 (t, 2H, J = 7.6 Hz), 2.38 (t, 2H, J = 7.2 Hz), 2.46 (t, 2H, J = 6.0 Hz), 2.78 (s, 2H), 2.89 (s, 2H), 3.23 (t, 2H, J = 5.6 Hz), 3.46 (t, 2H, J = 5.6 Hz), 3.53–3.54 (m, 24H), 3.65 (t, 2H, J = 6.0 Hz), 3.76 (s, 1H), 3.94 (s, 1H); 13C NMR (CDCl3): δ 23.6, 25.2, 27.1, 27.5, 32.8, 35.6, 38.8, 40.8, 41.4, 66.5, 68.8, 69.3, 69.4, 69.46, 69.53, 169.8, 174.1, 176.9; HRMS [C26H51N3O10S + Na]+: calc. 620.319, found: 620.311.

3.7. General procedure for synthesis of peptides/glycopeptides

Rink Amide AM Resin (33 µmol loading) was used for all peptides. All common amino acids were coupled on an Applied Biosystems 433A Peptide Synthesizer using HBTU–HOBt–DIEA with NMP as solvent and piperidine for Fmoc-deprotection. Coupling of the glycosylated amino acid (1R,2S)-N-Fmoc-1-amino-1-carboxypropan-2-yl 2,3,4,6-tetra-O-acetyl-β-d-galactoporanosyl-(1→3)-2-acetamido-4,6-O-benzylidene-2-deoxy-α-d-galactopyranoside and the linker 17 to the N-terminus was performed by dissolving the acid in DMF followed by addition of HOBt (2 eq.) and DIPCDI (2 eg.). This was stirred for 30 min, added to the resin and the resin was then shaken for 5–6 h. Another portion of HOBt (5 eq.) and DIPCDI (5 eq.) were added and the resin was shaken for an additional 18 h. Removal of the sugar and thioacetate groups was performed on the resin by treatment with 10% hydrazine hydrate in EtOH for 18 h. The peptides were cleaved by treating the resin with a solution of 2.5% 1,2-ethanedithiol and 2.5% water in TFA for 2 h. The peptide was then precipitated by pouring the TFA solution into ice-cooled Et2O (10 mL) where upon the peptides precipitated. The obtained mixture was centrifuged and the organic phase removed. The residue was purified by HPLC (water–acetonitrile gradient, each containing 0.1% TFA) yielding the pure peptide/glycopeptides as a white solid.

3.7.1 HS-linker-TSSASTGHATPLPVTD (11)

Following the general procedure yielded 11 (43 mg, 63%). 1H NMR (selected data) (CDCl3): δ 2.38 (t, 2H, J = 6.8 Hz), 2.47 (t, 2H, J = 6.0 Hz), 2.71 (dt, 1H, J = 7.6 Hz, J = 16.8 Hz), 2.76 (dt, 1H, J = 5.6 Hz, J = 17.2 Hz), 2.98 (dd, 1H, J = 8.4 Hz, J = 15.6 Hz), 3.14 (dd, 1H, J = 5.6 Hz, J = 15.6 Hz), 3.23 (t, 2H, J = 5.6 Hz), 3.46 (t, 2H, J = 5.2 Hz), 3.65 (t, 2H, J = 6.0 Hz), 3.87 (s, 2H), 4.57 (dd, 1H, J = 5.2 Hz, J = 7.6 Hz), 7.14 (d, 1H, J = 1.2 Hz), 8.47 (d, 1H, J = 1.2 Hz); HRMS [C88H150N21O34S]: calc. 2077.04, found: 2077.04.

3.7.2. HS-linker-TSSASTGHAT(Galβ1→3GalNAcα)PLPVTD (12)

Following the general procedure yielded 13 (38 mg, 47%). 1H NMR (selected data) (CDCl3): δ 2.38 (t, 2H, J = 6.8 Hz), 2.47 (t, 2H, J = 6.0 Hz), 2.71 (dt, 1H, J = 7.6 Hz, J = 16.8 Hz), 2.76 (dt, 1H, J = 5.6 Hz, J = 17.2 Hz), 2.98 (dd, 1H, J = 8.4 Hz, J = 15.6 Hz), 3.14 (dd, 1H, J = 5.6 Hz, J = 15.6 Hz), 3.23 (t, 2H, J = 5.6 Hz), 3.35 (t, 1H, J = 2.0 Hz, J = 7.6 Hz), 3.46 (t, 2H, J = 5.2 Hz), 3.65 (t, 2H, J = 6.0 Hz), 3.87 (s, 2H), 4.74 (d, 1H, J = 3.6 Hz, GalNAc H-1I), 7.15 (d, 1H, J = 1.2 Hz), 8.47 (d, 1H, J = 1.2 Hz); HRMS [C102H173N22O44S]: calc. 2442.170, found: 2442.170.

3.7.3. HS-linker-TSSAST(Galβ1→3GalNAcα)GHATPLPVTD (13)

Following the general procedure yielded 12 (41 mg, 51%). 1H NMR (selected data) (CDCl3): δ 2.38 (t, 2H, J = 6.8 Hz), 2.47 (t, 2H, J = 6.0 Hz), 2.71 (dt, 1H, J = 7.6 Hz, J = 16.8 Hz), 2.76 (dt, 1H, J = 5.6 Hz, J = 17.2 Hz), 2.98 (dd, 1H, J = 8.4 Hz, J = 15.6 Hz), 3.14 (dd, 1H, J = 5.6 Hz, J = 15.6 Hz), 3.23 (t, 2H, J = 5.6 Hz), 3.35 (t, 1H, J = 2.0 Hz, J = 7.6 Hz), 3.46 (t, 2H, J = 5.2 Hz), 3.65 (t, 2H, J = 6.0 Hz), 3.87 (s, 2H), 4.78 (d, 1H, J = 3.6 Hz, GalNAc H-1I), 7.15 (d, 1H, J = 1.2 Hz), 8.47 (d, 1H, J = 1.2 Hz); HRMS [C102H173N22O44S]: calc. 2442.170, found: 2442.170.

Supplementary Material

01

Acknowledgments

The authors thank Anil Patri, Jeff Clogston and Jiwen Zheng of the Nanotechnology Characterization Laboratory (DLS data and analysis) and Kunio Nagashima and Jason de la Cruz of the Image Analysis Laboratory (TEM data and analysis) for their work and helpful discussions. Part of this work was supported by the Intramural Research Program of the National Cancer Institute.

Footnotes

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