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. 1985 Dec;56(3):717–722. doi: 10.1128/jvi.56.3.717-722.1985

Biological and biochemical differences between variants of spleen focus-forming virus can be localized to a region containing the 3' end of the envelope gene.

S Ruscetti, L Wolff
PMCID: PMC252641  PMID: 2999427

Abstract

Two variants of the spleen focus-forming virus (SFFV), SFFVP and SFFVA, induce acute erythroleukemia in mice but differ in their effects on erythroid cells as well as in the posttranslational modification of the product of their envelope genes. To localize the region of the SFFV envelope gene responsible for these differences, we utilized a recombinant virus containing the 3' half of the SFFVP env gene, where the vast majority of the differences between SFFVP and SFFVA reside, and the SFFVP long terminal repeat (LTR) on an SFFVA background. Analysis of the recombinant virus indicates that it is capable of inducing all of the biological effects previously associated with SFFVP, including the ability to proliferate and differentiate without the need for erythropoietin. In addition, the env gene product of the recombinant virus can be detected on the cell surface, a property previously associated only with SFFVP. Although the recombinant virus also contains LTR sequences from SFFVP, we do not believe it is likely that the four LTR nucleotides that are unique to SFFVP are responsible for the biological or biochemical differences observed. These results strengthen the argument that the SFFVP env gene product acts at the cell surface to alter the hormonal requirements for erythroid cell growth and differentiation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bestwick R. K., Boswell B. A., Kabat D. Molecular cloning of biologically active Rauscher spleen focus-forming virus and the sequences of its env gene and long terminal repeat. J Virol. 1984 Sep;51(3):695–705. doi: 10.1128/jvi.51.3.695-705.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Downward J., Yarden Y., Mayes E., Scrace G., Totty N., Stockwell P., Ullrich A., Schlessinger J., Waterfield M. D. Close similarity of epidermal growth factor receptor and v-erb-B oncogene protein sequences. Nature. 1984 Feb 9;307(5951):521–527. doi: 10.1038/307521a0. [DOI] [PubMed] [Google Scholar]
  3. Hankins W. D., Kaminchik J., Luna J. Transformation of adult and fetal hemopoietic tissues with RNA tumor viruses. Prog Clin Biol Res. 1983;134:245–261. [PubMed] [Google Scholar]
  4. Hankins W. D., Krantz S. B. In vitro expression of erythroid differentiation induced by Friend polycythaemia virus. Nature. 1975 Feb 27;253(5494):731–732. doi: 10.1038/253731a0. [DOI] [PubMed] [Google Scholar]
  5. Hankins W. D., Troxler D. Polycythemia- and anemia-inducing erythroleukemia viruses exhibit differential erythroid transforming effects in vitro. Cell. 1980 Dec;22(3):693–699. doi: 10.1016/0092-8674(80)90545-0. [DOI] [PubMed] [Google Scholar]
  6. Horoszewicz J. S., Leong S. S., Carter W. A. Friend leukemia: rapid development of erythropoietin-independent hematopoietic precursors. J Natl Cancer Inst. 1975 Jan;54(1):265–267. doi: 10.1093/jnci/54.1.265. [DOI] [PubMed] [Google Scholar]
  7. Kaminchik J., Hankins W. D., Ruscetti S. K., Linemeyer D. L., Scolnick E. M. Molecular cloning of biologically active proviral DNA of the anemia-inducing strain of spleen focus-forming virus. J Virol. 1982 Dec;44(3):922–931. doi: 10.1128/jvi.44.3.922-931.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Krystal G. A simple microassay for erythropoietin based on 3H-thymidine incorporation into spleen cells from phenylhydrazine treated mice. Exp Hematol. 1983 Aug;11(7):649–660. [PubMed] [Google Scholar]
  9. Liao S. K., Axelrad A. A. Erythropoietin-independent erythroid colony formation in vitro by hemopoietic cells of mice infected with friend virus. Int J Cancer. 1975 Mar 15;15(3):467–482. doi: 10.1002/ijc.2910150313. [DOI] [PubMed] [Google Scholar]
  10. Linemeyer D. L., Menke J. G., Ruscetti S. K., Evans L. H., Scolnick E. M. Envelope gene sequences which encode the gp52 protein of spleen focus-forming virus are required for the induction of erythroid cell proliferation. J Virol. 1982 Jul;43(1):223–233. doi: 10.1128/jvi.43.1.223-233.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Linemeyer D. L., Ruscetti S. K., Scolnick E. M., Evans L. H., Duesberg P. H. Biological activity of the spleen focus-forming virus is encoded by a molecularly cloned subgenomic fragment of spleen focus-forming virus DNA. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1401–1405. doi: 10.1073/pnas.78.3.1401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MacDonald M. E., Reynolds F. H., Jr, Van de Ven W. J., Stephenson J. R., Mak T. W., Bernstein A. Anemia- and polycythemia-inducing isolates of Friend spleen focus-forming virus. Biological and molecular evidence for two distinct viral genomes. J Exp Med. 1980 Jun 1;151(6):1477–1492. doi: 10.1084/jem.151.6.1477. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pinter A., Honnen W. J., Li J. S. Studies with inhibitors of oligosaccharide processing indicate a functional role for complex sugars in the transport and proteolysis of Friend mink cell focus-inducing murine leukemia virus envelope proteins. Virology. 1984 Jul 15;136(1):196–210. doi: 10.1016/0042-6822(84)90259-9. [DOI] [PubMed] [Google Scholar]
  14. Polonoff E., Machida C. A., Kabat D. Glycosylation and intracellular transport of membrane glycoproteins encoded by murine leukemia viruses. Inhibition by amino acid analogues and by tunicamycin. J Biol Chem. 1982 Dec 10;257(23):14023–14028. [PubMed] [Google Scholar]
  15. Ruscetti S. K., Feild J. A., Scolnick E. M. Polycythaemia- and anaemia-inducing strains of spleen focus-forming virus differ in post-translational processing of envelope-related glycoproteins. Nature. 1981 Dec 17;294(5842):663–665. doi: 10.1038/294663a0. [DOI] [PubMed] [Google Scholar]
  16. Ruscetti S. K., Linemeyer D., Feild J., Troxler D., Scolnick E. M. Characterization of a protein found in cells infected with the spleen focus-forming virus that shares immunological cross-reactivity with the gp70 found in mink cell focus-inducing virus particles. J Virol. 1979 Jun;30(3):787–798. doi: 10.1128/jvi.30.3.787-798.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ruscetti S., Wolff L. Spleen focus-forming virus: relationship of an altered envelope gene to the development of a rapid erythroleukemia. Curr Top Microbiol Immunol. 1984;112:21–44. doi: 10.1007/978-3-642-69677-0_2. [DOI] [PubMed] [Google Scholar]
  18. Ruta M., Kabat D. Plasma membrane glycoproteins encoded by cloned Rauscher and Friend spleen focus-forming viruses. J Virol. 1980 Sep;35(3):844–853. doi: 10.1128/jvi.35.3.844-853.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Southern P. J., Berg P. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J Mol Appl Genet. 1982;1(4):327–341. [PubMed] [Google Scholar]
  20. Srinivas R. V., Compans R. W. Glycosylation and intracellular transport of spleen focus-forming virus glycoproteins. Virology. 1983 Mar;125(2):274–286. doi: 10.1016/0042-6822(83)90201-5. [DOI] [PubMed] [Google Scholar]
  21. Srinivas R. V., Compans R. W. Membrane association and defective transport of spleen focus-forming virus glycoproteins. J Biol Chem. 1983 Dec 10;258(23):14718–14724. [PubMed] [Google Scholar]
  22. Steinheider G., Seidel H. J., Kreja L. Comparison of the biological effects of anemia inducing and polycythemia inducing Friend virus complex. Experientia. 1979 Sep 15;35(9):1173–1175. doi: 10.1007/BF01963269. [DOI] [PubMed] [Google Scholar]
  23. Stephenson J. R., Axelrad A. A., McLeod D. L., Shreeve M. M. Induction of colonies of hemoglobin-synthesizing cells by erythropoietin in vitro. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1542–1546. doi: 10.1073/pnas.68.7.1542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Tambourin P. E., Wendling F., Jasmin C., Smadja-Joffe F. The physiopathology of Friend leukemia. Leuk Res. 1979;3(3):117–129. doi: 10.1016/0145-2126(79)90009-2. [DOI] [PubMed] [Google Scholar]
  25. Wolff L., Hubbert N., Ruscetti S. Structural analysis of the spleen focus-forming virus envelope gene product. Virology. 1984 Mar;133(2):376–385. doi: 10.1016/0042-6822(84)90403-3. [DOI] [PubMed] [Google Scholar]
  26. Wolff L., Kaminchik J., Hankins W. D., Ruscetti S. K. Sequence comparisons of the anemia- and polycythemia-inducing strains of Friend spleen focus-forming virus. J Virol. 1985 Feb;53(2):570–578. doi: 10.1128/jvi.53.2.570-578.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wolff L., Ruscetti S. Malignant transformation of erythroid cells in vivo by introduction of a nonreplicating retrovirus vector. Science. 1985 Jun 28;228(4707):1549–1552. doi: 10.1126/science.2990034. [DOI] [PubMed] [Google Scholar]

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