Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1985 Dec;56(3):951–957. doi: 10.1128/jvi.56.3.951-957.1985

Effects of sodium n-butyrate on entry into S phase in resting rat 3Y1 cells infected with simian virus 40.

T Mitsudomi, G Kimura
PMCID: PMC252669  PMID: 2999448

Abstract

In quiescent rat 3Y1 fibroblasts infected with simian virus 40 (SV40), sodium butyrate elongated the time lag before entry into S phase in a concentration-dependent fashion. In spite of the elongated time lags, SV40-infected cells entered S phase in a very synchronous mode, irrespective of the butyrate concentrations. The elongated time lag seemed to be at least partially due to a delayed synthesis and a delayed accumulation of large T antigen caused by butyrate. The entry into S phase was also delayed even when butyrate was added to the cultures after expression of T antigen to an extent sufficient for untreated cells to enter S phase. This suggests that butyrate may also inhibit a cellular event(s) that is required for entry into S phase after expression of the T antigen. In contrast, serum-stimulated cells were more sensitive to butyrate with respect to entry into S phase than SV40-infected cells, and the distribution of the time lag among cell populations increased (i.e., asynchrony in entry into S phase increased) with an increase in the butyrate concentration.

Full text

PDF
951

Images in this article

954Image
on p.954
954Image
on p.954
955Image
on p.955

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altenburg B. C., Via D. P., Steiner S. H. Modification of the phenotype of murine sarcoma virus-transformed cells by sodium butyrate. Effects on morphology and cytoskeletal elements. Exp Cell Res. 1976 Oct 15;102(2):223–231. doi: 10.1016/0014-4827(76)90036-7. [DOI] [PubMed] [Google Scholar]
  2. Candido E. P., Reeves R., Davie J. R. Sodium butyrate inhibits histone deacetylation in cultured cells. Cell. 1978 May;14(1):105–113. doi: 10.1016/0092-8674(78)90305-7. [DOI] [PubMed] [Google Scholar]
  3. Chamberlain J. P. Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate. Anal Biochem. 1979 Sep 15;98(1):132–135. doi: 10.1016/0003-2697(79)90716-4. [DOI] [PubMed] [Google Scholar]
  4. D'Anna J. A., Tobey R. A., Gurley L. R. Concentration-dependent effects of sodium butyrate in Chinese hamster cells: cell-cycle progression, inner-histone acetylation, histone H1 dephosphorylation, and induction of an H1-like protein. Biochemistry. 1980 Jun 10;19(12):2656–2671. doi: 10.1021/bi00553a019. [DOI] [PubMed] [Google Scholar]
  5. Daniell E., Burg J. L., Fedor M. J. DNA and histone synthesis of butyrate-inhibited BSC-1 cells infected with SV40. Virology. 1982 Jan 15;116(1):196–206. doi: 10.1016/0042-6822(82)90413-5. [DOI] [PubMed] [Google Scholar]
  6. Daniell E. Cells inhibited by n-butyrate support adenovirus replication. Virology. 1980 Dec;107(2):514–519. doi: 10.1016/0042-6822(80)90318-9. [DOI] [PubMed] [Google Scholar]
  7. Fallon R. J., Cox R. P. Cell cycle analysis of sodium butyrate and hydroxyurea, inducers of ectopic hormone production in HeLa cells. J Cell Physiol. 1979 Aug;100(2):251–262. doi: 10.1002/jcp.1041000206. [DOI] [PubMed] [Google Scholar]
  8. Gaudray P., Rassoulzadegan M., Cuzin F. Expression of simian virus 40 early genes in transformed rat cells is correlated with maintenance of the transformed phenotype. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4987–4991. doi: 10.1073/pnas.75.10.4987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ghosh M. K., Cox R. P. Production of human chorionic gonadotropin in HeLa cell cultures. Nature. 1976 Feb 5;259(5542):416–417. doi: 10.1038/259416a0. [DOI] [PubMed] [Google Scholar]
  10. Ginsburg E., Salomon D., Sreevalsan T., Freese E. Growth inhibition and morphological changes caused by lipophilic acids in mammalian cells. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2457–2461. doi: 10.1073/pnas.70.8.2457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gurney E. G., Harrison R. O., Fenno J. Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct sublcasses of large T antigen and for similarities among nonviral T antigens. J Virol. 1980 Jun;34(3):752–763. doi: 10.1128/jvi.34.3.752-763.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hagopian H. K., Riggs M. G., Swartz L. A., Ingram V. M. Effect of n-butyrate on DNA synthesis in chick fibroblasts and HeLa cells. Cell. 1977 Nov;12(3):855–860. doi: 10.1016/0092-8674(77)90284-7. [DOI] [PubMed] [Google Scholar]
  13. Hand R. Functions of T antigens of SV40 and polyomavirus. Biochim Biophys Acta. 1981 Aug 31;651(1):1–24. doi: 10.1016/0304-419x(81)90002-0. [DOI] [PubMed] [Google Scholar]
  14. Hawkes R., Niday E., Gordon J. A dot-immunobinding assay for monoclonal and other antibodies. Anal Biochem. 1982 Jan 1;119(1):142–147. doi: 10.1016/0003-2697(82)90677-7. [DOI] [PubMed] [Google Scholar]
  15. Iseki S., Baserga R. Effect of butyrate on adenovirus infection in semipermissive cells. Virology. 1983 Jan 15;124(1):188–191. doi: 10.1016/0042-6822(83)90304-5. [DOI] [PubMed] [Google Scholar]
  16. Kawasaki S., Diamond L., Baserga R. Induction of cellular deoxyribonucleic acid synthesis in butyrate-treated cells by simian virus 40 deoxyribonucleic acid. Mol Cell Biol. 1981 Nov;1(11):1038–1047. doi: 10.1128/mcb.1.11.1038. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kimura G., Itagaki A., Summers J. Rat cell line 3y1 and its virogenic polyoma- and sv40- transformed derivatives. Int J Cancer. 1975 Apr 15;15(4):694–706. doi: 10.1002/ijc.2910150419. [DOI] [PubMed] [Google Scholar]
  18. Leder A., Leder P. Butyric acid, a potent inducer of erythroid differentiation in cultured erythroleukemic cells. Cell. 1975 Jul;5(3):319–322. doi: 10.1016/0092-8674(75)90107-5. [DOI] [PubMed] [Google Scholar]
  19. Mitsudomi T., Kimura G. Abortive transformation of temperature-sensitive mutants of rat 3Y1 cells by simian virus 40: effect of cellular arrest states on entry into S phase and cellular proliferation. J Cell Physiol. 1985 Jun;123(3):353–360. doi: 10.1002/jcp.1041230310. [DOI] [PubMed] [Google Scholar]
  20. Okuda A., Shimura H., Kimura G. Abortive transformation of rat 3Y1 cells by simian virus 40: viral function overcoming inhibition of cellular proliferation under various conditions of culture. Virology. 1984 Feb;133(1):35–45. doi: 10.1016/0042-6822(84)90423-9. [DOI] [PubMed] [Google Scholar]
  21. Prasad K. N., Sinha P. K. Effect of sodium butyrate on mammalian cells in culture: a review. In Vitro. 1976 Feb;12(2):125–132. doi: 10.1007/BF02796360. [DOI] [PubMed] [Google Scholar]
  22. Riggs M. G., Whittaker R. G., Neumann J. R., Ingram V. M. n-Butyrate causes histone modification in HeLa and Friend erythroleukaemia cells. Nature. 1977 Aug 4;268(5619):462–464. doi: 10.1038/268462a0. [DOI] [PubMed] [Google Scholar]
  23. Rubenstein P., Sealy L., Marshall S., Chalkley R. Cellular protein synthesis and inhibition of cell division are independent of butyrate-induced histone hyperacetylation. Nature. 1979 Aug 23;280(5724):692–693. doi: 10.1038/280692a0. [DOI] [PubMed] [Google Scholar]
  24. Tamura H., Shimura H., Kimura G. Antiserum to SV40 T antigens from a rabbit inoculated with autochthonous cells transformed in vitro by SV40. Gan. 1981 Oct;72(5):769–771. [PubMed] [Google Scholar]
  25. Tjian R., Fey G., Graessmann A. Biological activity of purified simian virus 40 T antigen proteins. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1279–1283. doi: 10.1073/pnas.75.3.1279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Todaro G. J., Takemoto K. K. "Rescued" SV40: increased transforming efficiency in mouse and human cells. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1031–1037. doi: 10.1073/pnas.62.4.1031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Vidali G., Boffa L. C., Mann R. S., Allfrey V. G. Reversible effects of Na-butyrate on histone acetylation. Biochem Biophys Res Commun. 1978 May 15;82(1):223–227. doi: 10.1016/0006-291x(78)90599-5. [DOI] [PubMed] [Google Scholar]
  29. Wawra E., Pöckl E., Müllner E., Wintersberger E. Effect of sodium butyrate on induction of cellular and viral DNA syntheses in polyoma virus-infected mouse kidney cells. J Virol. 1981 Jun;38(3):973–981. doi: 10.1128/jvi.38.3.973-981.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wright J. A. Morphology and growth rate changes in Chinese hamster cells cultured in presence of sodium butyrate. Exp Cell Res. 1973 Apr;78(2):456–460. doi: 10.1016/0014-4827(73)90091-8. [DOI] [PubMed] [Google Scholar]
  31. Yamada K., Kimura G. Formation of proliferative tetraploid cells after treatment of diploid cells with sodium butyrate in rat 3Y1 fibroblasts. J Cell Physiol. 1985 Jan;122(1):59–63. doi: 10.1002/jcp.1041220110. [DOI] [PubMed] [Google Scholar]
  32. Yanishevsky R. M., Stein G. H. Regulation of the cell cycle in eukaryotic cells. Int Rev Cytol. 1981;69:223–259. doi: 10.1016/s0074-7696(08)62324-4. [DOI] [PubMed] [Google Scholar]
  33. Zouzias D., Basilico C. T-antigen expression in proliferating and non-proliferating simian virus 40-transformed mouse cells. J Virol. 1979 Jun;30(3):711–719. doi: 10.1128/jvi.30.3.711-719.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES