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. 1985 Dec;56(3):1049–1052. doi: 10.1128/jvi.56.3.1049-1052.1985

Effect of human alpha A interferon on influenza virus replication in MDBK cells.

R M Ransohoff, P A Maroney, D P Nayak, T M Chambers, T W Nilsen
PMCID: PMC252685  PMID: 4068143

Abstract

To determine the molecular mechanism whereby interferon induces resistance to influenza virus, we began an investigation of influenza virus replication in MDBK cells treated with recombinant human alpha A interferon. Negative- and positive-strand virus-specific RNA accumulation was monitored by blot hybridization with cloned probes. Primary transcription (transcription of infecting viral negative strands by the virion-associated polymerase) was inhibited by interferon treatment of MDBK cells. At moderate levels of interferon treatment (10 U/ml), this inhibition was restricted to transcripts of polymerase genes, whereas at higher levels of interferon treatment (50 U/ml), accumulation of all primary transcripts was markedly inhibited. Secondary transcripts and viral negative strands did not accumulate to any significant extent in interferon-treated MDBK cells. These results suggest that interferon-induced mechanisms which inhibit influenza virus replication in MDBK cells act at the level of primary transcription.

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Selected References

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