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. 1986 Jan;57(1):138–144. doi: 10.1128/jvi.57.1.138-144.1986

Functional interactions of the simian virus 40 core origin of replication with flanking regulatory sequences.

A L DeLucia, S Deb, K Partin, P Tegtmeyer
PMCID: PMC252708  PMID: 3001340

Abstract

We constructed a matched set of plasmids to investigate the interactions of essential core sequences of the simian virus 40 replication origin with flanking regulatory sequences. Deletions of either T-antigen-binding region I or the 21-base-pair repeated promoter elements reduced replication to 50 to 70% of wild-type levels. The simultaneous deletion of both regions decreased replication to less than 5% of wild-type levels. Thus, the double deletion greatly amplified the defects of the single deletions. We conclude that region I and the 21-base-pair repeats have related rather than independent functions in DNA synthesis. Insertion of a synthetic region I or the adenovirus 2 major late promoter at the late side of isolated core sequences in place of the 21-base-pair repeats failed to restore replication. In contrast, insertion of a single 72-base-pair enhancer element stimulated replication of the core origin more than fivefold. Thus, three distinct regulatory elements appear to facilitate core DNA replication by related mechanisms. Flanking sequences have only a small direct effect on T-antigen binding to naked core DNA. Possible mechanisms of action include the regulation of transcription or of chromatin structure.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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