Table 2.
Experimentally measured 15N chemical shift (Figure 4) and 1H-15N dipolar coupling (Figure 5) values of SKP and SA peptides embedded in mechanically aligned POPC and DMPC lipid bilayers at 30°C.
| Peptide | V3 | L5 | V9 | L11 | L12 | A15 |
|---|---|---|---|---|---|---|
| POPC (SKP or SA): | ||||||
| Chemical Shift (ppm) | 210±2 | 203±3 | 205±1.5 | 200±0.9 | 195±1 | 198±3 |
| Dipolar coupling (kHz) * | 9.5 | 7.2 | 7.0 | 9.3 | 7.7 | 8.2 |
| SKP in DMPC: | ||||||
| Chemical Shift (ppm) | 190±2 | 172±1.7 | 178±2 | 167±1 | 170±1 | 168±2 |
| Dipolar coupling (kHz)* | 8.5 | 3.5 | 3.0 | 8.0 | 5.0 | 7.0 |
| SA in DMPC: | ||||||
| Chemical Shift (ppm) | 200±2 | 185±1 | 192±1 | 181±1.4 | 180±1 | 178±2 |
| Dipolar coupling (kHz)* | 9.0 | 5.0 | 4.8 | 9.0 | 5.5 | 8.5 |
*
Based on line widths, errors in the reported dipolar couplings are estimated to be about ±0.3 to ±0.4 kHz.