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. 1986 Mar;57(3):983–991. doi: 10.1128/jvi.57.3.983-991.1986

Characterization of foot-and-mouth disease virus gene products with antisera against bacterially synthesized fusion proteins.

K Strebel, E Beck, K Strohmaier, H Schaller
PMCID: PMC252830  PMID: 3005640

Abstract

Defined segments of the cloned foot-and-mouth disease virus genome corresponding to all parts of the coding region were expressed in Escherichia coli as fusions to the N-terminal part of the MS2-polymerase gene under the control of the inducible lambda PL promoter. All constructs yielded large amounts of proteins, which were purified and used to raise sequence-specific antisera in rabbits. These antisera were used to identify the corresponding viral gene products in 35S-labeled extracts from foot-and-mouth disease virus gene products in the nucleotide sequence, to identify precursor-product relationships, and to detect several foot-and-mouth disease virus gene products not previously identified in vivo or in vitro.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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