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. 1986 Mar;57(3):1016–1022. doi: 10.1128/jvi.57.3.1016-1022.1986

trans activation of the latent Epstein-Barr virus (EBV) genome after transfection of the EBV DNA fragment.

K Takada, N Shimizu, S Sakuma, Y Ono
PMCID: PMC252835  PMID: 3005608

Abstract

Transfection of Epstein-Barr virus (EBV)-nonproducer Raji cells with the BamHI Z fragment of EBV DNA induced antigens that were detected with human antiserum against EBV-specific early antigens. Northern blot analysis of transfected cells revealed that one intense RNA band hybridized with the BamHI H and F fragments but not with the BamHI Z fragment. Cooperation between the BamHI H, F, and BamHI Z regions was also confirmed in baby hamster kidney cells that were cotransfected with both fragments. These results indicate that the transfected BamHI Z fragment of EBV DNA induces a trans-acting factor which activates the gene expression of the BamHI H and F region and that the BamHI Z region possibly plays an important role in the latency of EBV.

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