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. 1986 Apr;58(1):192–202. doi: 10.1128/jvi.58.1.192-202.1986

Temperature-sensitive mutants of frog virus 3: biochemical and genetic characterization.

V G Chinchar, A Granoff
PMCID: PMC252893  PMID: 3951023

Abstract

Nineteen frog virus 3 temperature-sensitive mutants were isolated after mutagenesis with nitrosoguanidine and assayed for viral DNA, RNA, and protein synthesis, as well as assembly site formation at permissive (25 degrees C) and nonpermissive (30 degrees C) temperatures. In addition, mutants were characterized for complementation by both quantitative and qualitative assays. Based on the genetic and biochemical data, the 19 mutants, along with 9 mutants isolated earlier, were ordered into four phenotypic classes which define defects in virion morphogenesis (class I), late mRNA synthesis (class II), viral assembly site formation (class III), and viral DNA synthesis (class IV). In addition, we used two-factor crosses to order 11 mutants, comprising 7 complementation groups, onto a linkage map spanning 77 recombination units.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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