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. 1986 Apr;58(1):223–224. doi: 10.1128/jvi.58.1.223-224.1986

Location of the Bacillus subtilis temperate bacteriophage phi 105 attP attachment site.

D M Ellis, D H Dean
PMCID: PMC252898  PMID: 3081735

Abstract

Chromosomal DNAs of lysogens of phi 105 and phi 105 DI:1t were digested with restriction enzymes EcoRI and HpaI and were probed with nick-translated mature phi 105 DNA. Altered bacteriophage-specific bands in the lysogens were detected, indicating that the phage integrates into the host chromosome at a single site, probably via a Campbell-type circular intermediate. The phage attachment site is centrally located in the phage genome and lies between the phage immunity region and the nonessential deletable region of phi 105.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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