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. 1986 May;58(2):508–512. doi: 10.1128/jvi.58.2.508-512.1986

Frequent partial deletion of human adult T-cell leukemia virus type I proviruses in experimental transmission: pattern and possible implication.

K Hiramatsu, H Yoshikura
PMCID: PMC252938  PMID: 3009864

Abstract

Human T-cell leukemia virus type I (HTLV I) propagated in human diploid fibroblast IMR90 was transmitted to human promyelocytic leukemia HL60 cells by coculture. Of 14 provirus-positive HL60 clones, five harbored only defective proviruses, five had defective proviruses in addition to full-sized HTLV I, and four had full-sized proviruses integrated in their chromosomes. The frequency of defective proviruses was unexpectedly high (41% of total proviruses). Analysis of the genomic structure of these defective proviruses revealed polarity of deletion, that is, preferred conservation of the 3' end of the proviral genome (pX and the 3' long terminal repeat). The implication of these findings are discussed with reference to the replication and pathogenesis of HTLV I.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clapham P., Nagy K., Cheingsong-Popov R., Exley M., Weiss R. A. Productive infection and cell-free transmission of human T-cell leukemia virus in a nonlymphoid cell line. Science. 1983 Dec 9;222(4628):1125–1127. doi: 10.1126/science.6316502. [DOI] [PubMed] [Google Scholar]
  2. Clarke M. F., Gelmann E. P., Reitz M. S., Jr Homology of human T-cell leukaemia virus envelope gene with class I HLA gene. Nature. 1983 Sep 1;305(5929):60–62. doi: 10.1038/305060a0. [DOI] [PubMed] [Google Scholar]
  3. Collins S. J., Ruscetti F. W., Gallagher R. E., Gallo R. C. Terminal differentiation of human promyelocytic leukemia cells induced by dimethyl sulfoxide and other polar compounds. Proc Natl Acad Sci U S A. 1978 May;75(5):2458–2462. doi: 10.1073/pnas.75.5.2458. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fung Y. K., Fadly A. M., Crittenden L. B., Kung H. J. On the mechanism of retrovirus-induced avian lymphoid leukosis: deletion and integration of the proviruses. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3418–3422. doi: 10.1073/pnas.78.6.3418. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hoshino H., Shimoyama M., Miwa M., Sugimura T. Detection of lymphocytes producing a human retrovirus associated with adult T-cell leukemia by syncytia induction assay. Proc Natl Acad Sci U S A. 1983 Dec;80(23):7337–7341. doi: 10.1073/pnas.80.23.7337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hughes S. H., Toyoshima K., Bishop J. M., Varmus H. E. Organization of the endogenous proviruses of chickens: implications for origin and expression. Virology. 1981 Jan 15;108(1):189–207. doi: 10.1016/0042-6822(81)90538-9. [DOI] [PubMed] [Google Scholar]
  7. Majors J. E., Varmus H. E. Nucleotide sequencing of an apparent proviral copy of env mRNA defines determinants of expression of the mouse mammary tumor virus env gene. J Virol. 1983 Sep;47(3):495–504. doi: 10.1128/jvi.47.3.495-504.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Meier E., Harmison G. G., Keene J. D., Schubert M. Sites of copy choice replication involved in generation of vesicular stomatitis virus defective-interfering particle RNAs. J Virol. 1984 Aug;51(2):515–521. doi: 10.1128/jvi.51.2.515-521.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nagy K., Clapham P., Cheingsong-Popov R., Weiss R. A. Human T-cell leukemia virus type I: induction of syncytia and inhibition by patients' sera. Int J Cancer. 1983 Sep 15;32(3):321–328. doi: 10.1002/ijc.2910320310. [DOI] [PubMed] [Google Scholar]
  10. Nichols W. W., Murphy D. G., Cristofalo V. J., Toji L. H., Greene A. E., Dwight S. A. Characterization of a new human diploid cell strain, IMR-90. Science. 1977 Apr 1;196(4285):60–63. doi: 10.1126/science.841339. [DOI] [PubMed] [Google Scholar]
  11. Olsen J. C., Swanstrom R. A new pathway in the generation of defective retrovirus DNA. J Virol. 1985 Dec;56(3):779–789. doi: 10.1128/jvi.56.3.779-789.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Seiki M., Hattori S., Hirayama Y., Yoshida M. Human adult T-cell leukemia virus: complete nucleotide sequence of the provirus genome integrated in leukemia cell DNA. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3618–3622. doi: 10.1073/pnas.80.12.3618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Seiki M., Hikikoshi A., Taniguchi T., Yoshida M. Expression of the pX gene of HTLV-I: general splicing mechanism in the HTLV family. Science. 1985 Jun 28;228(4707):1532–1534. doi: 10.1126/science.2990031. [DOI] [PubMed] [Google Scholar]
  14. Shields A., Witte W. N., Rothenberg E., Baltimore D. High frequency of aberrant expression of Moloney murine leukemia virus in clonal infections. Cell. 1978 Jul;14(3):601–609. doi: 10.1016/0092-8674(78)90245-3. [DOI] [PubMed] [Google Scholar]
  15. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  16. Voynow S. L., Coffin J. M. Evolutionary variants of Rous sarcoma virus: large deletion mutants do not result from homologous recombination. J Virol. 1985 Jul;55(1):67–78. doi: 10.1128/jvi.55.1.67-78.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wong-Staal F., Hahn B., Manzari V., Colombini S., Franchini G., Gelmann E. P., Gallo R. C. A survey of human leukaemias for sequences of a human retrovirus. Nature. 1983 Apr 14;302(5909):626–628. doi: 10.1038/302626a0. [DOI] [PubMed] [Google Scholar]
  18. Yoshida M., Hattori S., Seiki M. Molecular biology of human T-cell leukemia virus associated with adult T-cell leukemia. Curr Top Microbiol Immunol. 1985;115:157–175. doi: 10.1007/978-3-642-70113-9_11. [DOI] [PubMed] [Google Scholar]
  19. Yoshida M., Seiki M., Yamaguchi K., Takatsuki K. Monoclonal integration of human T-cell leukemia provirus in all primary tumors of adult T-cell leukemia suggests causative role of human T-cell leukemia virus in the disease. Proc Natl Acad Sci U S A. 1984 Apr;81(8):2534–2537. doi: 10.1073/pnas.81.8.2534. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Yoshikura H., Nishida J., Yoshida M., Kitamura Y., Takaku F., Ikeda S. Isolation of HTLV derived from Japanese adult T-cell leukemia patients in human diploid fibroblast strain IMR90 and the biological characters of the infected cells. Int J Cancer. 1984 Jun 15;33(6):745–749. doi: 10.1002/ijc.2910330606. [DOI] [PubMed] [Google Scholar]

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