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. 1986 May;58(2):569–577. doi: 10.1128/jvi.58.2.569-577.1986

Specific proteins synthesized during the viral lytic cycle in vaccinia virus-infected HeLa cells: analysis by high-resolution, two-dimensional gel electrophoresis.

L Carrasco, R Bravo
PMCID: PMC252946  PMID: 3701923

Abstract

The proteins synthesized in vaccinia-infected HeLa cells have been analyzed at different times after infection by using two-dimensional gel electrophoresis. Vaccinia-infected cells present up to 198 polypeptides (138 acidic, isoelectric focusing; 60 basic, nonequilibrium pH gradient electrophoresis) not detected in control cells. Cells infected in the presence of cycloheximide show 81 additional polypeptides after cycloheximide removal, resulting in a total estimate of 279 proteins induced after vaccinia infection. The glycoproteins made at various times postinfection were also analyzed. At least 13 proteins labeled with [3H]glucosamine were detected in vaccinia-infected HeLa cells.

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Selected References

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  1. Chen X., Rösel J., Dunker R., Goldschmidt R., Maurer-Schultze B., Jungwirth C. Reversible inhibition of poxvirus replication by cycloheximide during the early phase of infection. Virology. 1983 Jan 30;124(2):308–317. doi: 10.1016/0042-6822(83)90347-1. [DOI] [PubMed] [Google Scholar]
  2. Essani K., Dales S. Biogenesis of vaccinia: evidence for more than 100 polypeptides in the virion. Virology. 1979 Jun;95(2):385–394. doi: 10.1016/0042-6822(79)90493-8. [DOI] [PubMed] [Google Scholar]
  3. Esteban M., Metz D. H. Early virus protein synthesis in vaccinia virus-infected cells. J Gen Virol. 1973 May;19(2):201–206. doi: 10.1099/0022-1317-19-2-201. [DOI] [PubMed] [Google Scholar]
  4. JOKLIK W. K. The preparation and characteristics of highly purified radioactively labelled poxvirus. Biochim Biophys Acta. 1962 Aug 20;61:290–301. doi: 10.1016/0926-6550(62)90091-9. [DOI] [PubMed] [Google Scholar]
  5. Kilpatrick D., Rouhandeh H. The polypeptides of monkeypox virus: I. Analysis of the polypeptide synthesis of MPV by SDS-PAGE and by two-dimensional electrophoresis. Virology. 1981 Apr 30;110(2):455–465. doi: 10.1016/0042-6822(81)90076-3. [DOI] [PubMed] [Google Scholar]
  6. Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
  7. Mahr A., Roberts B. E. Arrangement of late RNAs transcribed from a 7.1-kilobase EcoRI vaccinia virus DNA fragment. J Virol. 1984 Feb;49(2):510–520. doi: 10.1128/jvi.49.2.510-520.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mahr A., Roberts B. E. Organization of six early transcripts synthesized from a vaccinia virus EcoRI DNA fragment. J Virol. 1984 Feb;49(2):497–509. doi: 10.1128/jvi.49.2.497-509.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Morgan J. R., Roberts B. E. Organization of RNA transcripts from a vaccinia virus early gene cluster. J Virol. 1984 Aug;51(2):283–297. doi: 10.1128/jvi.51.2.283-297.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  11. O'Farrell P. Z., Goodman H. M., O'Farrell P. H. High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell. 1977 Dec;12(4):1133–1141. doi: 10.1016/0092-8674(77)90176-3. [DOI] [PubMed] [Google Scholar]
  12. Pennington T. H. Vaccinia virus polypeptide synthesis: sequential appearance and stability of pre- and post-replicative polypeptides. J Gen Virol. 1974 Dec;25(3):433–444. doi: 10.1099/0022-1317-25-3-433. [DOI] [PubMed] [Google Scholar]
  13. Vafai A., Rouhandeh H. Analysis of Yaba tumor poxvirus-induced proteins in infected cells by two-dimensional gel electrophoresis. Virology. 1982 Jul 15;120(1):65–76. doi: 10.1016/0042-6822(82)90007-1. [DOI] [PubMed] [Google Scholar]
  14. Wittek R., Hänggi M., Hiller G. Mapping of a gene coding for a major late structural polypeptide on the vaccinia virus genome. J Virol. 1984 Feb;49(2):371–378. doi: 10.1128/jvi.49.2.371-378.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wittek R., Richner B., Hiller G. Mapping of the genes coding for the two major vaccinia virus core polypeptides. Nucleic Acids Res. 1984 Jun 25;12(12):4835–4848. doi: 10.1093/nar/12.12.4835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Zöller B., Krempien U., Jungwirth C. Synthesis of early vaccinia-virus-specific enzymes under conditions of immediate early gene expression. Eur J Biochem. 1984 Sep 17;143(3):589–591. doi: 10.1111/j.1432-1033.1984.tb08410.x. [DOI] [PubMed] [Google Scholar]

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