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. Author manuscript; available in PMC: 2008 Sep 5.
Published in final edited form as: Biochemistry. 2007 Jul 27;46(33):9495–9506. doi: 10.1021/bi700555g

Figure 1.

Figure 1

Traditional scheme for the catalytic cycle of ALDH. E-SH denotes the enzyme with Cys302 as the nucleophile and the line to NAD indicates that it is non-covalently bound prior to reacting the substrate. Reaction I: Nucleophilic activation and attack on the substrate by Cys302 to give a thiohemiacetal intermediate. Reaction II: Hydride transfer from the intermediate to NAD to form NADH and a thioester. Reaction III: Hydrolysis of the thioester with release of the carboxylic acid. Reaction IV: Release of NADH.