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. 1986 Jun;58(3):951–954. doi: 10.1128/jvi.58.3.951-954.1986

End structure and mechanism of packaging of bacteriophage T4 DNA.

A Kalinski, L W Black
PMCID: PMC253004  PMID: 3701933

Abstract

We analyzed by restriction enzyme digestion the end structure of T4 phage DNA by comparing mature, concatemeric, first-packaged, and incompletely packaged DNAs. The structure of mature DNA was also studied using 3' end labeling with terminal transferase. Our data support the hypothesis that T4 DNA packaging is not initiated at specific packaging initiation sequences on the concatemeric precursor (cos or pac site mechanisms) but by a different packaging mechanism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allet B., Jeppesen P. G., Katagiri K. J., Delius H. Mapping the DNA fragments produced by cleavage by lambda DNA with endonuclease RI. Nature. 1973 Jan 12;241(5385):120–123. doi: 10.1038/241120a0. [DOI] [PubMed] [Google Scholar]
  2. Black L. W. In vitro packaging of bacteriophage T4 DNA. Virology. 1981 Aug;113(1):336–344. doi: 10.1016/0042-6822(81)90160-4. [DOI] [PubMed] [Google Scholar]
  3. Black L. W., Silverman D. J. Model for DNA packaging into bacteriophage T4 heads. J Virol. 1978 Nov;28(2):643–655. doi: 10.1128/jvi.28.2.643-655.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bächi B., Arber W. Physical mapping of BglII, BamHI, EcoRI, HindIII and PstI restriction fragments of bacteriophage P1 DNA. Mol Gen Genet. 1977 Jun 24;153(3):311–324. doi: 10.1007/BF00431596. [DOI] [PubMed] [Google Scholar]
  5. Hascoet E. C., Pretorius G. H., Coetzee W. F. Proteus mirabilis phage 5006M: restriction maps of genome in relation to headful packaging. Virology. 1982 Nov;123(1):1–7. doi: 10.1016/0042-6822(82)90289-6. [DOI] [PubMed] [Google Scholar]
  6. Hsiao C. L., Black L. W. DNA packaging and the pathway of bacteriophage T4 head assembly. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3652–3656. doi: 10.1073/pnas.74.9.3652. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jackson E. N., Jackson D. A., Deans R. J. EcoRI analysis of bacteriophage P22 DNA packaging. J Mol Biol. 1978 Jan 25;118(3):365–388. doi: 10.1016/0022-2836(78)90234-6. [DOI] [PubMed] [Google Scholar]
  8. Jackson E. N., Miller H. I., Adams M. L. EcoRI restriction endonuclease cleavage site map of bacteriophage P22DNA. J Mol Biol. 1978 Jan 25;118(3):347–363. doi: 10.1016/0022-2836(78)90233-4. [DOI] [PubMed] [Google Scholar]
  9. Kaplan D. A., Nierlich D. P. Cleavage of Nonglucosylated Bacteriophage T4 deoxyribonucleic acid by Restriction Endonuclease Eco RI. J Biol Chem. 1975 Mar 25;250(6):2395–2397. [PubMed] [Google Scholar]
  10. Laemmli U. K., Favre M. Maturation of the head of bacteriophage T4. I. DNA packaging events. J Mol Biol. 1973 Nov 15;80(4):575–599. doi: 10.1016/0022-2836(73)90198-8. [DOI] [PubMed] [Google Scholar]
  11. Luftig R. B., Wood W. B., Okinaka R. Bacteriophage T4 head morphogenesis. On the nature of gene 49-defective heads and their role as intermediates. J Mol Biol. 1971 May 14;57(3):555–573. doi: 10.1016/0022-2836(71)90109-4. [DOI] [PubMed] [Google Scholar]
  12. MacHattie L. A., Ritchie D. A., Thomas C. A., Jr, Richardson C. C. Terminal repetition in permuted T2 bacteriophage DNA molecules. J Mol Biol. 1967 Feb 14;23(3):355–363. doi: 10.1016/s0022-2836(67)80110-4. [DOI] [PubMed] [Google Scholar]
  13. Mizuuchi K., Kemper B., Hays J., Weisberg R. A. T4 endonuclease VII cleaves holliday structures. Cell. 1982 Jun;29(2):357–365. doi: 10.1016/0092-8674(82)90152-0. [DOI] [PubMed] [Google Scholar]
  14. Mosig G., Ehring R., Schliewen W., Bock S. The patterns of recombination and segregation in terminal regions of T4DNA molecules. Mol Gen Genet. 1971;113(1):51–91. doi: 10.1007/BF00335007. [DOI] [PubMed] [Google Scholar]
  15. Mosig G., Ghosal D., Bock S. Interactions between the maturation protein gp17 and the single-stranded DNA binding protein gp32 initiate DNA packaging and compete with initiation of secondary DNA replication forks in phage T4. Prog Clin Biol Res. 1981;64:139–150. [PubMed] [Google Scholar]
  16. Murialdo H., Becker A. Head morphogenesis of complex double-stranded deoxyribonucleic acid bacteriophages. Microbiol Rev. 1978 Sep;42(3):529–576. doi: 10.1128/mr.42.3.529-576.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ramsey N., Ritchie D. A. Uncoupling of initiation site cleavage from subsequent headful cleavages in bacteriophage T1 DNA packaging. Nature. 1983 Jan 20;301(5897):264–266. doi: 10.1038/301264a0. [DOI] [PubMed] [Google Scholar]
  18. Roychoudhury R., Wu R. Terminal transferase-catalyzed addition of nucleotides to the 3' termini of DNA. Methods Enzymol. 1980;65(1):43–62. doi: 10.1016/s0076-6879(80)65009-5. [DOI] [PubMed] [Google Scholar]
  19. Silverstein J. L., Goldberg E. B. T4 DNA injection. II. Protection of entering DNA from host exonuclease V. Virology. 1976 Jul 1;72(1):212–223. doi: 10.1016/0042-6822(76)90324-x. [DOI] [PubMed] [Google Scholar]
  20. Streisinger G., Emrich J., Stahl M. M. Chromosome structure in phage t4, iii. Terminal redundancy and length determination. Proc Natl Acad Sci U S A. 1967 Feb;57(2):292–295. doi: 10.1073/pnas.57.2.292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tye B. K., Huberman J. A., Botstein D. Non-random circular permutation of phage P22 DNA. J Mol Biol. 1974 Jan 5;85(4):501–528. doi: 10.1016/0022-2836(74)90312-x. [DOI] [PubMed] [Google Scholar]

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