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. 1986 Jun;58(3):970–974. doi: 10.1128/jvi.58.3.970-974.1986

Nuclease mechanism of the avian retrovirus pp32 endonuclease.

D P Grandgenett, A C Vora, R Swanstrom, J C Olsen
PMCID: PMC253009  PMID: 3009900

Abstract

In vivo, the inferred circular retrovirus DNA precursor to the provirus contains two long terminal repeats (LTRs) in tandem. We studied the site-specific nicking of supercoiled DNA that contains tandem copies of avian retrovirus LTR DNA in vitro by using purified avian myeloblastosis virus pp32 endonuclease, Mg2+, and viral DNA substrates containing different LTR circle junction sequences. The results confirmed our previous observation that the pp32 protein generates two nicks, one in either viral DNA strand, each 2 nucleotides from the circle junction site. The specificity of nicking by pp32 was unchanged over an eight-fold range of protein concentration and with different avian retrovirus LTR circle junction substrates. These data are consistent with models which propose a role for the endonuclease in removal of two nucleotides from the LTR termini on integration of viral DNA in vivo.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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