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. 1986 Jul;59(1):163–167. doi: 10.1128/jvi.59.1.163-167.1986

A conserved cis-acting sequence in the 5' leader of avian sarcoma virus RNA is required for packaging.

R A Katz, R W Terry, A M Skalka
PMCID: PMC253052  PMID: 3012114

Abstract

The deletion of a conserved sequence of ca. 30 nucleotides in the 5' noncoding leader region of an avian sarcoma virus DNA clone resulted in a loss of infectivity after transfection of chicken embryo fibroblasts. Genetic and biochemical analysis of a representative mutant demonstrated that the env gene was expressed normally. Thus, viral RNA transcription, splicing, and translation were not impaired. The amount of mutant viral RNA encapsidated into virions, however, was severely reduced despite the presence of helper-virus. We conclude that the deleted sequence is an essential cis-acting packaging signal.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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