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Journal of Virology logoLink to Journal of Virology
. 1986 Aug;59(2):301–307. doi: 10.1128/jvi.59.2.301-307.1986

A novel retroviruslike family in mouse DNA.

A Itin, E Keshet
PMCID: PMC253079  PMID: 3735487

Abstract

In the course of structural analysis of VL30 DNA elements, a recombinant retroviruslike element was encountered that contained non-VL30 long terminal repeats (LTRs) flanking internal VL30 sequences. With the aid of this novel LTR sequence probe, we cloned several DNA elements that were apparently members of a new retroviruslike family. A particular DNA element representative of this family (designated GLN) was characterized. It was approximately 8 kilobase pairs long and contained LTRs that are 430 base pairs long. It possessed an unusual primer-binding site sequence that corresponds to tRNAGln and a polypurine tract primer that is adjacent to the 3' LTR. The nucleotide sequences of the LTRs and their adjacent regions (which together housed all cis-acting retroviral functions) were different from those of known retroviruses and retroviruslike families. The comparison of three different GLN LTR sequences revealed a marked heterogeneity of U3 sequences relative to the homogeneity of R and U5 sequences. We estimate that approximately 20 to 50 copies of GLN elements are dispersed in all species of mice. GLN-related LTRs, however, are present in a much higher copy number (1,000 to 1,500 per genome). Nucleotide sequences that are more distantly related to GLN DNA are present in multiple copies in DNAs of other rodents but not in nonrodent genomes.

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Selected References

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  1. Besmer P., Olshevsky U., Baltimore D., Dolberg D., Fan H. Virus-like 30S RNA in mouse cells. J Virol. 1979 Mar;29(3):1168–1176. doi: 10.1128/jvi.29.3.1168-1176.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Callahan R., Chiu I. M., Wong J. F., Tronick S. R., Roe B. A., Aaronson S. A., Schlom J. A new class of endogenous human retroviral genomes. Science. 1985 Jun 7;228(4704):1208–1211. doi: 10.1126/science.2408338. [DOI] [PubMed] [Google Scholar]
  3. Callahan R., Kuff E. L., Lueders K. K., Birkenmeier E. Genetic relationship between the Mus cervicolor M432 retrovirus and the Mus Musculus intracisternal type A particle. J Virol. 1981 Dec;40(3):901–911. doi: 10.1128/jvi.40.3.901-911.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hodgson C. P., Elder P. K., Ono T., Foster D. N., Getz M. J. Structure and expression of mouse VL30 genes. Mol Cell Biol. 1983 Dec;3(12):2221–2231. doi: 10.1128/mcb.3.12.2221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Howk R. S., Troxler D. H., Lowy D., Duesberg P. H., Scolnick E. M. Identification of a 30S RNA with properties of a defective type C virus in murine cells. J Virol. 1978 Jan;25(1):115–123. doi: 10.1128/jvi.25.1.115-123.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Itin A., Keshet E. Apparent recombinants between virus-liKE (VL30) and murine leukemia virus-related sequences in mouse DNA. J Virol. 1983 Jul;47(1):178–184. doi: 10.1128/jvi.47.1.178-184.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Itin A., Keshet E. Primer binding sites corresponding to several tRNA species are present in DNAs of different members of the same retrovirus-like gene family (VL30). J Virol. 1985 Apr;54(1):236–239. doi: 10.1128/jvi.54.1.236-239.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Keshet E., Itin A. Patterns of genomic distribution and sequence heterogeneity of a murine "retrovirus-like" multigene family. J Virol. 1982 Jul;43(1):50–58. doi: 10.1128/jvi.43.1.50-58.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Keshet E., Shaul Y., Kaminchik J., Aviv H. Heterogeneity of "virus-like" genes encoding retrovirus-associated 30S RNA and their organization within the mouse genome. Cell. 1980 Jun;20(2):431–439. doi: 10.1016/0092-8674(80)90629-7. [DOI] [PubMed] [Google Scholar]
  10. Khan A. S., Martin M. A. Endogenous murine leukemia proviral long terminal repeats contain a unique 190-base-pair insert. Proc Natl Acad Sci U S A. 1983 May;80(9):2699–2703. doi: 10.1073/pnas.80.9.2699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kuff E. L., Leuders K. K., Ozer H. L., Wivel N. A. Some structural and antigenic properties of intracisternal A particles occurring in mouse tumors (complement fixation-immunodiffusion-neuroblastoma-plasma-cell tumor). Proc Natl Acad Sci U S A. 1972 Jan;69(1):218–222. doi: 10.1073/pnas.69.1.218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Larson R., Messing J. Apple II software for M13 shotgun DNA sequencing. Nucleic Acids Res. 1982 Jan 11;10(1):39–49. doi: 10.1093/nar/10.1.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lueders K. K., Kuff E. L. Intracisternal A-particle genes: identification in the genome of Mus musculus and comparison of multiple isolates from a mouse gene library. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3571–3575. doi: 10.1073/pnas.77.6.3571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mager D. L., Henthorn P. S. Identification of a retrovirus-like repetitive element in human DNA. Proc Natl Acad Sci U S A. 1984 Dec;81(23):7510–7514. doi: 10.1073/pnas.81.23.7510. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mann R., Mulligan R. C., Baltimore D. Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus. Cell. 1983 May;33(1):153–159. doi: 10.1016/0092-8674(83)90344-6. [DOI] [PubMed] [Google Scholar]
  16. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  17. Nikbakht K. N., Ou C. Y., Boone L. R., Glover P. L., Yang W. K. Nucleotide sequence analysis of endogenous murine leukemia virus-related proviral clones reveals primer-binding sites for glutamine tRNA. J Virol. 1985 Jun;54(3):889–893. doi: 10.1128/jvi.54.3.889-893.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. O'Connell C., O'Brien S., Nash W. G., Cohen M. ERV3, a full-length human endogenous provirus: chromosomal localization and evolutionary relationships. Virology. 1984 Oct 30;138(2):225–235. doi: 10.1016/0042-6822(84)90347-7. [DOI] [PubMed] [Google Scholar]
  19. Propst F., Vande Woude G. F. A novel transposon-like repeat interrupted by an LTR element occurs in a cluster of B1 repeats in the mouse c-mos locus. Nucleic Acids Res. 1984 Nov 26;12(22):8381–8392. doi: 10.1093/nar/12.22.8381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rotman G., Itin A., Keshet E. 'Solo' large terminal repeats (LTR) of an endogenous retrovirus-like gene family (VL30) in the mouse genome. Nucleic Acids Res. 1984 Mar 12;12(5):2273–2282. doi: 10.1093/nar/12.5.2273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Schmidt M., Wirth T., Kröger B., Horak I. Structure and genomic organization of a new family of murine retrovirus-related DNA sequences (MuRRS). Nucleic Acids Res. 1985 May 24;13(10):3461–3470. doi: 10.1093/nar/13.10.3461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Steele P. E., Rabson A. B., Bryan T., Martin M. A. Distinctive termini characterize two families of human endogenous retroviral sequences. Science. 1984 Aug 31;225(4665):943–947. doi: 10.1126/science.6089336. [DOI] [PubMed] [Google Scholar]
  23. Temin H. M. Origin of retroviruses from cellular moveable genetic elements. Cell. 1980 Oct;21(3):599–600. doi: 10.1016/0092-8674(80)90420-1. [DOI] [PubMed] [Google Scholar]
  24. Varmus H. E. Form and function of retroviral proviruses. Science. 1982 May 21;216(4548):812–820. doi: 10.1126/science.6177038. [DOI] [PubMed] [Google Scholar]
  25. Watanabe S., Temin H. M. Encapsidation sequences for spleen necrosis virus, an avian retrovirus, are between the 5' long terminal repeat and the start of the gag gene. Proc Natl Acad Sci U S A. 1982 Oct;79(19):5986–5990. doi: 10.1073/pnas.79.19.5986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Yang J. A., Tai L. W., Agris P. F., Gehrke C. W., Wong T. W. The nucleotide sequence of a major glutamine tRNA from rat liver. Nucleic Acids Res. 1983 Apr 11;11(7):1991–1996. doi: 10.1093/nar/11.7.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]

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