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. Author manuscript; available in PMC: 2008 Sep 7.
Published in final edited form as: Biochemistry. 2006 Mar 21;45(11):3588–3597. doi: 10.1021/bi0525369

Fig. 5. Influence of the E1 fragment and the synthetic peptides mimicking knobs “A” and “B” on the stability of the fibrin-derived D dimer.

Fig. 5

Solid and dashed curves represent fluorescence-detected melting of the dimeric D-D fragment at 0.16 μM and the D-D:E1 complex at 0.12 μM, respectively. Melting of 0.16 μM D-D in the presence of a 100-fold molar excess of Gly-Pro-Arg-Pro or Gly-Pro-Arg-Pro and Gly-His-Arg-Pro are shown by open and filled circles, respectively, while that in the presence of a 1000-fold excess of Gly-Pro-Arg-Pro is shown by open triangles. All experiments were performed in 50 mM glycine buffer, pH 8.6, with 0.5 mM Ca2+.