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. 1988 Apr;62(4):1474–1477. doi: 10.1128/jvi.62.4.1474-1477.1988

Evidence that transcriptional control is the major mechanism of regulation for the glycoprotein D gene in herpes simplex virus type 1-infected cells.

I L Smith 1, R M Sandri-Goldin 1
PMCID: PMC253166  PMID: 2831409

Abstract

In this report, we demonstrate a close correlation between the levels of steady-state mRNA, polysome-associated RNA, and the rate of protein synthesis for glycoprotein D, a beta-gamma product of herpes simplex virus type 1, and for the model beta (ICP8), beta-gamma (glycoprotein B), and gamma (glycoprotein C) gene products included in this study as controls. No evidence was found for posttranscriptional control of expression of these products. We conclude from these results that the major regulatory control for these genes during herpes simplex virus type 1 infection is transcriptional.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arsenakis M., Hubenthal-Voss J., Campadelli-Fiume G., Pereira L., Roizman B. Construction and properties of a cell line constitutively expressing the herpes simplex virus glycoprotein B dependent on functional alpha 4 protein synthesis. J Virol. 1986 Nov;60(2):674–682. doi: 10.1128/jvi.60.2.674-682.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cathala G., Savouret J. F., Mendez B., West B. L., Karin M., Martial J. A., Baxter J. D. A method for isolation of intact, translationally active ribonucleic acid. DNA. 1983;2(4):329–335. doi: 10.1089/dna.1983.2.329. [DOI] [PubMed] [Google Scholar]
  3. Chua C. C., Carter T. H., St Jeor S. Transcription of the human cytomegalovirus genome in productively infected cells. J Gen Virol. 1981 Sep;56(Pt 1):1–11. doi: 10.1099/0022-1317-56-1-1. [DOI] [PubMed] [Google Scholar]
  4. Demarchi J. M. Human cytomegalovirus DNA: restriction enzyme cleavage maps and map locations for immediate-early, early, and late RNAs. Virology. 1981 Oct 15;114(1):23–38. doi: 10.1016/0042-6822(81)90249-x. [DOI] [PubMed] [Google Scholar]
  5. Geballe A. P., Spaete R. R., Mocarski E. S. A cis-acting element within the 5' leader of a cytomegalovirus beta transcript determines kinetic class. Cell. 1986 Sep 12;46(6):865–872. doi: 10.1016/0092-8674(86)90068-1. [DOI] [PubMed] [Google Scholar]
  6. Godowski P. J., Knipe D. M. Transcriptional control of herpesvirus gene expression: gene functions required for positive and negative regulation. Proc Natl Acad Sci U S A. 1986 Jan;83(2):256–260. doi: 10.1073/pnas.83.2.256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goins W. F., Stinski M. F. Expression of a human cytomegalovirus late gene is posttranscriptionally regulated by a 3'-end-processing event occurring exclusively late after infection. Mol Cell Biol. 1986 Dec;6(12):4202–4213. doi: 10.1128/mcb.6.12.4202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Holland L. E., Anderson K. P., Shipman C., Jr, Wagner E. K. Viral DNA synthesis is required for the efficient expression of specific herpes simplex virus type 1 mRNA species. Virology. 1980 Feb;101(1):10–24. doi: 10.1016/0042-6822(80)90479-1. [DOI] [PubMed] [Google Scholar]
  9. Holland T. C., Marlin S. D., Levine M., Glorioso J. Antigenic variants of herpes simplex virus selected with glycoprotein-specific monoclonal antibodies. J Virol. 1983 Feb;45(2):672–682. doi: 10.1128/jvi.45.2.672-682.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Honess R. W., Roizman B. Regulation of herpesvirus macromolecular synthesis. I. Cascade regulation of the synthesis of three groups of viral proteins. J Virol. 1974 Jul;14(1):8–19. doi: 10.1128/jvi.14.1.8-19.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Honess R. W., Roizman B. Regulation of herpesvirus macromolecular synthesis: sequential transition of polypeptide synthesis requires functional viral polypeptides. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1276–1280. doi: 10.1073/pnas.72.4.1276. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jennings S. R., Lippe P. A., Pauza K. J., Spear P. G., Pereira L., Tevethia S. S. Kinetics of expression of herpes simplex virus type 1-specific glycoprotein species on the surfaces of infected murine, simian, and human cells: flow cytometric analysis. J Virol. 1987 Jan;61(1):104–112. doi: 10.1128/jvi.61.1.104-112.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Johnson D. C., Spear P. G. Evidence for translational regulation of herpes simplex virus type 1 gD expression. J Virol. 1984 Aug;51(2):389–394. doi: 10.1128/jvi.51.2.389-394.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Johnson P. A., MacLean C., Marsden H. S., Dalziel R. G., Everett R. D. The product of gene US11 of herpes simplex virus type 1 is expressed as a true late gene. J Gen Virol. 1986 May;67(Pt 5):871–883. doi: 10.1099/0022-1317-67-5-871. [DOI] [PubMed] [Google Scholar]
  15. Jones P. C., Roizman B. Regulation of herpesvirus macromolecular synthesis. VIII. The transcription program consists of three phases during which both extent of transcription and accumulation of RNA in the cytoplasm are regulated. J Virol. 1979 Aug;31(2):299–314. doi: 10.1128/jvi.31.2.299-314.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Palmiter R. D. Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid. Biochemistry. 1974 Aug 13;13(17):3606–3615. doi: 10.1021/bi00714a032. [DOI] [PubMed] [Google Scholar]
  17. Powell K. L., Littler E., Purifoy D. J. Nonstructural proteins of herpes simplex virus. II. Major virus-specific DNa-binding protein. J Virol. 1981 Sep;39(3):894–902. doi: 10.1128/jvi.39.3.894-902.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sandri-Goldin R. M., Goldin A. L., Holland L. E., Glorioso J. C., Levine M. Expression of herpes simplex virus beta and gamma genes integrated in mammalian cells and their induction by an alpha gene product. Mol Cell Biol. 1983 Nov;3(11):2028–2044. doi: 10.1128/mcb.3.11.2028. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sandri-Goldin R. M., Sekulovich R. E., Leary K. The alpha protein ICP0 does not appear to play a major role in the regulation of herpes simplex virus gene expression during infection in tissue culture. Nucleic Acids Res. 1987 Feb 11;15(3):905–919. doi: 10.1093/nar/15.3.905. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Silver S., Roizman B. gamma 2-Thymidine kinase chimeras are identically transcribed but regulated a gamma 2 genes in herpes simplex virus genomes and as beta genes in cell genomes. Mol Cell Biol. 1985 Mar;5(3):518–528. doi: 10.1128/mcb.5.3.518. [DOI] [PMC free article] [PubMed] [Google Scholar]

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