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. 1988 May;62(5):1550–1557. doi: 10.1128/jvi.62.5.1550-1557.1988

Production of antibodies of predetermined specificity against herpes simplex virus DNA polymerase and their use in characterization of the enzyme.

M S Thomas 1, L M Banks 1, D J Purifoy 1, K L Powell 1
PMCID: PMC253181  PMID: 2833607

Abstract

Peptides from preselected regions of the herpes simplex virus DNA polymerase were used to generate monospecific antisera to defined regions of the enzyme. The antisera were used to localize the polymerase within the infected cell and to determine the time of synthesis during productive infection. Comparison with a neutralizing polyclonal antiserum was used to show the specificity of the peptide antisera. By using the antisera the stabilities of the DNA polymerase, the alkaline nuclease, and the major DNA-binding protein were determined, and the state of phosphorylation of the DNA polymerase was compared with each of these proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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