Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1988 May;62(5):1730–1735. doi: 10.1128/jvi.62.5.1730-1735.1988

DNA rearrangements in organ-specific variants of polyomavirus JC strain GS.

G Loeber 1, K Dörries 1
PMCID: PMC253214  PMID: 2833622

Abstract

Variants of JC virus (JCV) strain GS were isolated directly from the central nervous system (variant GS/B) and the kidney (variant GS/K) of a patient with progressive multifocal leukoencephalopathy and were cloned and sequenced. The genomes of the isolates were shown to be nearly identical in the nucleotide sequences of their protein-coding regions, suggesting that both had originated from a single infecting JCV genome. In contrast, the arrangement of the putative elements of transcriptional control revealed considerable differences. The tandemly repeated elements found twice within the enhancer region of JCV GS/B variant were not present in the GS/K variant. The missing elements were replaced by DNA segments containing simian virus 40 and adenovirus E1A core enhancer elements. These differences in the organ-specific GS variants suggest that rearrangements within elements of transcriptional control might be involved in altering the virus-cell interaction in the course of a JCV infection.

Full text

PDF
1730

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benton W. D., Davis R. W. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. doi: 10.1126/science.322279. [DOI] [PubMed] [Google Scholar]
  2. Coleman D. V., Wolfendale M. R., Daniel R. A., Dhanjal N. K., Gardner S. D., Gibson P. E., Field A. M. A prospective study of human polyomavirus infection in pregnancy. J Infect Dis. 1980 Jul;142(1):1–8. doi: 10.1093/infdis/142.1.1. [DOI] [PubMed] [Google Scholar]
  3. DeLucia A. L., Lewton B. A., Tjian R., Tegtmeyer P. Topography of simian virus 40 A protein-DNA complexes: arrangement of pentanucleotide interaction sites at the origin of replication. J Virol. 1983 Apr;46(1):143–150. doi: 10.1128/jvi.46.1.143-150.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Deb S., DeLucia A. L., Baur C. P., Koff A., Tegtmeyer P. Domain structure of the simian virus 40 core origin of replication. Mol Cell Biol. 1986 May;6(5):1663–1670. doi: 10.1128/mcb.6.5.1663. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Deyerle K. L., Cassill J. A., Subramani S. Analysis of the early regulatory region of the human papovavirus BK. Virology. 1987 May;158(1):181–193. doi: 10.1016/0042-6822(87)90252-2. [DOI] [PubMed] [Google Scholar]
  7. Dörries K., Johnson R. T., ter Meulen V. Detection of polyoma virus DNA in PML-brain tissue by (in situ) hybridization. J Gen Virol. 1979 Jan;42(1):49–57. doi: 10.1099/0022-1317-42-1-49. [DOI] [PubMed] [Google Scholar]
  8. Dörries K. Progressive multifocal leucoencephalopathy: analysis of JC virus DNA from brain and kidney tissue. Virus Res. 1984 Jan;1(1):25–38. doi: 10.1016/0168-1702(84)90032-7. [DOI] [PubMed] [Google Scholar]
  9. Dörries K., ter Meulen V. Progressive multifocal leucoencephalopathy: detection of papovavirus JC in kidney tissue. J Med Virol. 1983;11(4):307–317. doi: 10.1002/jmv.1890110406. [DOI] [PubMed] [Google Scholar]
  10. Feigenbaum L., Khalili K., Major E., Khoury G. Regulation of the host range of human papovavirus JCV. Proc Natl Acad Sci U S A. 1987 Jun;84(11):3695–3698. doi: 10.1073/pnas.84.11.3695. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Frisque R. J., Bream G. L., Cannella M. T. Human polyomavirus JC virus genome. J Virol. 1984 Aug;51(2):458–469. doi: 10.1128/jvi.51.2.458-469.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Grinnell B. W., Padgett B. L., Walker D. L. Comparison of infectious JC virus DNAs cloned from human brain. J Virol. 1983 Jan;45(1):299–308. doi: 10.1128/jvi.45.1.299-308.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hohn B. In vitro packaging of lambda and cosmid DNA. Methods Enzymol. 1979;68:299–309. doi: 10.1016/0076-6879(79)68021-7. [DOI] [PubMed] [Google Scholar]
  14. Kenney S., Natarajan V., Strike D., Khoury G., Salzman N. P. JC virus enhancer-promoter active in human brain cells. Science. 1984 Dec 14;226(4680):1337–1339. doi: 10.1126/science.6095453. [DOI] [PubMed] [Google Scholar]
  15. Li J. J., Peden K. W., Dixon R. A., Kelly T. Functional organization of the simian virus 40 origin of DNA replication. Mol Cell Biol. 1986 Apr;6(4):1117–1128. doi: 10.1128/mcb.6.4.1117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Martin J. D., Foster G. C. Multiple JC virus genomes from one patient. J Gen Virol. 1984 Aug;65(Pt 8):1405–1411. doi: 10.1099/0022-1317-65-8-1405. [DOI] [PubMed] [Google Scholar]
  17. Martin J. D., King D. M., Slauch J. M., Frisque R. J. Differences in regulatory sequences of naturally occurring JC virus variants. J Virol. 1985 Jan;53(1):306–311. doi: 10.1128/jvi.53.1.306-311.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Martin J. D., Padgett B. L., Walker D. L. Characterization of tissue culture-induced heterogeneity in DNAs of independent isolates of JC virus. J Gen Virol. 1983 Oct;64(Pt 10):2271–2280. doi: 10.1099/0022-1317-64-10-2271. [DOI] [PubMed] [Google Scholar]
  19. Matsuda M., Jona M., Yasui K., Nagashima K. Genetic characterization of JC virus Tokyo-1 strain, a variant oncogenic in rodents. Virus Res. 1987 Apr;7(2):159–168. doi: 10.1016/0168-1702(87)90077-3. [DOI] [PubMed] [Google Scholar]
  20. Messing J. New M13 vectors for cloning. Methods Enzymol. 1983;101:20–78. doi: 10.1016/0076-6879(83)01005-8. [DOI] [PubMed] [Google Scholar]
  21. Ondek B., Shepard A., Herr W. Discrete elements within the SV40 enhancer region display different cell-specific enhancer activities. EMBO J. 1987 Apr;6(4):1017–1025. doi: 10.1002/j.1460-2075.1987.tb04854.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Padgett B. L., Walker D. L. Virologic and serologic studies of progressive multifocal leukoencephalopathy. Prog Clin Biol Res. 1983;105:107–117. [PubMed] [Google Scholar]
  23. Padgett B. L., Walker D. L., ZuRhein G. M., Eckroade R. J., Dessel B. H. Cultivation of papova-like virus from human brain with progressive multifocal leucoencephalopathy. Lancet. 1971 Jun 19;1(7712):1257–1260. doi: 10.1016/s0140-6736(71)91777-6. [DOI] [PubMed] [Google Scholar]
  24. Rand K. H., Johnson K. P., Rubinstein L. J., Wolinsky J. S., Penney J. B., Walker D. L., Padgett B. L., Merigan T. C. Adenine arabinoside in the treatment of progressive multifocal leukoencephalopathy: use of virus-containing cells in the urine to assess response to therapy. Ann Neurol. 1977 May;1(5):458–462. doi: 10.1002/ana.410010509. [DOI] [PubMed] [Google Scholar]
  25. Rubinstein R., Pare N., Harley E. H. Structure and function of the transcriptional control region of nonpassaged BK virus. J Virol. 1987 May;61(5):1747–1750. doi: 10.1128/jvi.61.5.1747-1750.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Seif I., Khoury G., Dhar R. The genome of human papovavirus BKV. Cell. 1979 Dec;18(4):963–977. doi: 10.1016/0092-8674(79)90209-5. [DOI] [PubMed] [Google Scholar]
  28. Staden R. A computer program to enter DNA gel reading data into a computer. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):499–503. doi: 10.1093/nar/12.1part2.499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Stoner G. L., Ryschkewitsch C. F., Walker D. L., Webster H. D. JC papovavirus large tumor (T)-antigen expression in brain tissue of acquired immune deficiency syndrome (AIDS) and non-AIDS patients with progressive multifocal leukoencephalopathy. Proc Natl Acad Sci U S A. 1986 Apr;83(7):2271–2275. doi: 10.1073/pnas.83.7.2271. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Walker D. L., Padgett B. L. The epidemiology of human polyomaviruses. Prog Clin Biol Res. 1983;105:99–106. [PubMed] [Google Scholar]
  31. Weber F., de Villiers J., Schaffner W. An SV40 "enhancer trap" incorporates exogenous enhancers or generates enhancers from its own sequences. Cell. 1984 Apr;36(4):983–992. doi: 10.1016/0092-8674(84)90048-5. [DOI] [PubMed] [Google Scholar]
  32. Yang R. C., Wu R. BK virus DNA: complete nucleotide sequence of a human tumor virus. Science. 1979 Oct 26;206(4417):456–462. doi: 10.1126/science.228391. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES