Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1988 May;62(5):1827–1831. doi: 10.1128/jvi.62.5.1827-1831.1988

In vitro synthesis of infectious retroviral RNA.

F Flamant 1, J A Sorge 1
PMCID: PMC253241  PMID: 3357213

Abstract

A plasmid was constructed in which a T7 RNA polymerase promoter was placed upstream of a recombinant amphotropic retrovirus genome containing a selectable neomycin resistance gene. To test the infectivity of the RNA produced by T7 RNA polymerase in vitro, the RNA was microinjected into the nuclei of psi 2 packaging cells. Infectious particles conferring G418 resistance were released.

Full text

PDF
1827

Images in this article

1830Image
on p.1830
1830Image
on p.1830

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chamberlin M., McGrath J., Waskell L. New RNA polymerase from Escherichia coli infected with bacteriophage T7. Nature. 1970 Oct 17;228(5268):227–231. doi: 10.1038/228227a0. [DOI] [PubMed] [Google Scholar]
  2. Emerman M., Temin H. M. High-frequency deletion in recovered retrovirus vectors containing exogenous DNA with promoters. J Virol. 1984 Apr;50(1):42–49. doi: 10.1128/jvi.50.1.42-49.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hayward W. S., Neel B. G., Astrin S. M. Activation of a cellular onc gene by promoter insertion in ALV-induced lymphoid leukosis. Nature. 1981 Apr 9;290(5806):475–480. doi: 10.1038/290475a0. [DOI] [PubMed] [Google Scholar]
  4. Kane S. E., Beemon K. Precise localization of m6A in Rous sarcoma virus RNA reveals clustering of methylation sites: implications for RNA processing. Mol Cell Biol. 1985 Sep;5(9):2298–2306. doi: 10.1128/mcb.5.9.2298. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Keller G., Paige C., Gilboa E., Wagner E. F. Expression of a foreign gene in myeloid and lymphoid cells derived from multipotent haematopoietic precursors. Nature. 1985 Nov 14;318(6042):149–154. doi: 10.1038/318149a0. [DOI] [PubMed] [Google Scholar]
  6. Lobel L. I., Goff S. P. Reverse transcription of retroviral genomes: mutations in the terminal repeat sequences. J Virol. 1985 Feb;53(2):447–455. doi: 10.1128/jvi.53.2.447-455.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mann R., Mulligan R. C., Baltimore D. Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus. Cell. 1983 May;33(1):153–159. doi: 10.1016/0092-8674(83)90344-6. [DOI] [PubMed] [Google Scholar]
  8. Sorge J., Wright D., Erdman V. D., Cutting A. E. Amphotropic retrovirus vector system for human cell gene transfer. Mol Cell Biol. 1984 Sep;4(9):1730–1737. doi: 10.1128/mcb.4.9.1730. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Stacey D. W., Allfrey V. G., Hanafusa H. Microinjection analysis of envelope-glycoprotein messenger activities of avian leukosis viral RNAs. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1614–1618. doi: 10.1073/pnas.74.4.1614. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Stacey D. W. Microinjection of mRNA and other macromolecules into living cells. Methods Enzymol. 1981;79(Pt B):76–88. doi: 10.1016/s0076-6879(81)79016-5. [DOI] [PubMed] [Google Scholar]
  11. Stacey D. W. Microinjection studies of retroviral polynucleotides. Curr Top Microbiol Immunol. 1986;123:23–37. doi: 10.1007/978-3-642-70810-7_2. [DOI] [PubMed] [Google Scholar]
  12. Teich N. M., Weiss R. A., Martin G. R., Lowy D. R. Virus infection of murine teratocarcinoma stem cell lines. Cell. 1977 Dec;12(4):973–982. doi: 10.1016/0092-8674(77)90162-3. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES