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. Author manuscript; available in PMC: 2008 Sep 8.
Published in final edited form as: Neuroscience. 2007 Feb 27;146(2):833–840. doi: 10.1016/j.neuroscience.2007.01.036

Fig. 3.

Fig. 3

Effect of LIF and BMP4 on neuronal survival and morphology after 42 hours in culture. Control cultures contained BDNF, NT3 and serum. Compare these with cultures that additionally contained LIF, BMP4 or LIF+BMP4. A). Inclusion of either LIF, BMP4 or LIF+BMP4 increased survival over that in control cultures. B) LIF cultures had more bipolar neurons than control cultures. C) There were more monopolar neurons in cultures containing BMP4 or LIF+BMP4 than in control; and D) BMP4 increased the numbers of neurons with no neurites of control levels. Numbers represent the averages ± SEM of the means from 3 experiments, 2–4 wells per condition per experiment. Neurons in each well are normalized to surviving βIII Tubulin +cells/cochlea as described in the methods (left axis) and their survival is related to the total number of spiral ganglion neurons in the newborn cochlea on the right Y axis. The p values compare the LIF, BMP4 or LIF+BMP4 conditions to the control cultures. *p<0.05; **p<0.01; ***p<0.001.