Figure 4.

Strains deleted for GRX1 or GRX2 are sensitive to reactive oxygen species. Sensitivity to menadione, hydrogen peroxide, tert-butyl hydroperoxide, and diamide was determined by spotting strains on YEPD plates containing various concentrations of oxidants. Cultures of wild-type, grx1, grx2, and grx1 grx2 double mutant strains were grown to exponential phase (A₆₀₀ = 1) and into stationary phase in YEPD medium, adjusted to an A₆₀₀ of 3.0 and 0.3 before spotting 10-μl aliquots onto appropriate plates. Plates were incubated at 30°C for 3 d before scoring growth.