Table 3.
Glutathione reductase activity and cellular redox state in glutaredoxin mutants
| Strain | Glr activity (nmol·min−1·mg−1) | Thiol contenta (μmol/mg) | Total GSHb (nmol/107 cells) | GSSGc (pmol/107 cells) | GSSG (%) |
|---|---|---|---|---|---|
| wt | 152 ± 8 | 243 ± 63 | 3.2 ± 0.1 | 47.6 ± 3.4 | 1.5 |
| grx1 | 158 ± 18 | 212 ± 11 | 3.0 ± 0.1 | 43.4 ± 1.4 | 1.5 |
| grx2 | 135 ± 20 | 210 ± 17 | 2.5 ± 0.6 | 50.4 ± 10.5 | 2 |
| grx1 grx2 | 149 ± 0 | 227 ± 18 | 3.0 ± 0.1 | 51.8 ± 9.1 | 1.7 |
Data are the means of duplicate experiments.
a
Total thiol content includes both protein and GSH.
b
Total GSH is the equivalent of GSH + 2GSSG.
c
Percent GSSG is the portion of GSSG present in the cell relative to the amount of total GSH.