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. 1988 Jun;62(6):2191–2195. doi: 10.1128/jvi.62.6.2191-2195.1988

Regeneration of herpesviruses from molecularly cloned subgenomic fragments.

M van Zijl 1, W Quint 1, J Briaire 1, T de Rover 1, A Gielkens 1, A Berns 1
PMCID: PMC253328  PMID: 2835520

Abstract

The ability to manipulate the genomes of herpesviruses is of eminent importance for obtaining insight into gene function and regulation of gene expression of these complex viruses. Here we report the use of in vivo overlap recombination to generate pseudorabies virus mutants. Cotransfection of up to five overlapping cloned subgenomic fragments, which together constitute the entire genomic information of pseudorabies virus, results in the efficient reconstitution of virus. This allows the efficient introduction of multiple well-defined mutations in herpesvirus genomes in a single step, without any selection or screening for a particular phenotype.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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