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. 1986 Dec;60(3):943–949. doi: 10.1128/jvi.60.3.943-949.1986

Failure to detect polyalbumin-binding sites on the woodchuck hepatitis virus surface antigen: implications for the pathogenesis of hepatitis B virus in humans.

C J Pohl, P J Cote, R H Purcell, J L Gerin
PMCID: PMC253330  PMID: 3783821

Abstract

Binding sites for polymerized albumin on hepatitis B virus components were reported in human hepatitis B virus chronic carriers predominantly with active viral replication (HB e antigen positive). The presence of comparable albumin-binding sites in the woodchuck hepatitis virus (WHV) model was examined on WHV components obtained from woodchucks with active viral replication (DNA polymerase positive). Binding sites for polymerized woodchuck serum albumin were not detected on the intact WHV virion, on 22-nm woodchuck hepatitis surface antigen (WHsAg), or on WHsAg polypeptides. Woodchuck albumin was not detected in purified 22-nm WHsAg, and anti-albumin antibodies were not detected in WHV chronic-carrier woodchucks. Our results in the WHV model argue against a role for viral polyalbumin-binding sites in tissue- and host-specific virus infectivity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alberti A., Pontisso P., Schiavon E., Realdi G. An antibody which precipitates Dane particles in acute hepatitis type B: relation to receptor sites which bind polymerized human serum albumin on virus particles. Hepatology. 1984 Mar-Apr;4(2):220–226. doi: 10.1002/hep.1840040209. [DOI] [PubMed] [Google Scholar]
  2. Cote P. J., Engle R. E., Langer C. A., Ponzetto A., Gerin J. L. Antigenic analysis of woodchuck hepatitis virus surface antigen with site-specific radioimmunoassays. J Virol. 1984 Mar;49(3):701–708. doi: 10.1128/jvi.49.3.701-708.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Day J. F., Thornburg R. W., Thorpe S. R., Baynes J. W. Nonenzymatic glucosylation of rat albumin. Studies in vitro and in vivo. J Biol Chem. 1979 Oct 10;254(19):9394–9400. [PubMed] [Google Scholar]
  4. Feitelson M. A., Marion P. L., Robinson W. S. The nature of polypeptides larger in size than the major surface antigen components of hepatitis b and like viruses in ground squirrels, woodchucks, and ducks. Virology. 1983 Oct 15;130(1):76–90. doi: 10.1016/0042-6822(83)90119-8. [DOI] [PubMed] [Google Scholar]
  5. Fraker P. J., Speck J. C., Jr Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril. Biochem Biophys Res Commun. 1978 Feb 28;80(4):849–857. doi: 10.1016/0006-291x(78)91322-0. [DOI] [PubMed] [Google Scholar]
  6. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gerin J. L., Faust R. M., Holland P. V. Biophysical characterization of the adr subtype of hepatitis B antigen and preparation of anti-r sera in rabbits. J Immunol. 1975 Jul;115(1):100–105. [PubMed] [Google Scholar]
  8. Gilja B. K., Kasambalides E. J., Bressler R. S., Thung S. N., Gerber M. A. Secretion of polyalbumin receptors in vitro. J Med Virol. 1985 Apr;15(4):335–341. doi: 10.1002/jmv.1890150403. [DOI] [PubMed] [Google Scholar]
  9. Hansson B. G., Purcell R. H. Sites that bind polymerized albumin on hepatitis B surface antigen particles: detection by radioimmunoassay. Infect Immun. 1979 Oct;26(1):125–130. doi: 10.1128/iai.26.1.125-130.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Heermann K. H., Goldmann U., Schwartz W., Seyffarth T., Baumgarten H., Gerlich W. H. Large surface proteins of hepatitis B virus containing the pre-s sequence. J Virol. 1984 Nov;52(2):396–402. doi: 10.1128/jvi.52.2.396-402.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Imai M., Yanase Y., Nojiri T., Miyakawa Y., Mayumi M. A receptor for polymerized human and chimpanzee albumins on hepatitis B virus particles co-occurring with HBeAg. Gastroenterology. 1979 Feb;76(2):242–247. [PubMed] [Google Scholar]
  12. Ionescu-Matiu I., Sanchez Y., Hollinger F. B., Melnick J. L., Dreesman G. R. Presence of a receptor for albumin on individual HBsAg polypeptides and on HBsAg produced by a hepatoma cell line. J Med Virol. 1980;6(2):175–178. doi: 10.1002/jmv.1890060211. [DOI] [PubMed] [Google Scholar]
  13. Kaplan P. M., Greenman R. L., Gerin J. L., Purcell R. H., Robinson W. S. DNA polymerase associated with human hepatitis B antigen. J Virol. 1973 Nov;12(5):995–1005. doi: 10.1128/jvi.12.5.995-1005.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kasambalides E. J., Gilja B. K., Gerber M. A. Effects of glycosylation inhibitors on the expression of polyalbumin receptors by hepatitis B surface antigen produced in vitro. J Gen Virol. 1985 Nov;66(Pt 11):2443–2451. doi: 10.1099/0022-1317-66-11-2443. [DOI] [PubMed] [Google Scholar]
  15. Kodama K., Ogasawara N., Yoshikawa H., Murakami S. Nucleotide sequence of a cloned woodchuck hepatitis virus genome: evolutional relationship between hepadnaviruses. J Virol. 1985 Dec;56(3):978–986. doi: 10.1128/jvi.56.3.978-986.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  17. Lenkei R., Babeş V. T., Mustea A., Dobre I. Deeper insight into HBsAg--anti-albumin antibody correlations. J Med Virol. 1979;4(2):137–145. doi: 10.1002/jmv.1890040208. [DOI] [PubMed] [Google Scholar]
  18. Lenkei R., Onica D., Ghetie V. Receptors for polymerized albumin on liver cells. Experientia. 1977 Aug 15;33(8):1046–1047. doi: 10.1007/BF01945961. [DOI] [PubMed] [Google Scholar]
  19. Milich D. R., Gottfried T. D., Vyas G. N. Characterization of the interaction between polymerized human albumin and hepatitis B surface antigen. Gastroenterology. 1981 Aug;81(2):218–225. [PubMed] [Google Scholar]
  20. O'Neill S. P. Interaction of hepatitis B surface antigen with polymerized human serum albumin. J Med Virol. 1979;4(3):177–185. doi: 10.1002/jmv.1890040304. [DOI] [PubMed] [Google Scholar]
  21. Okamoto H., Imai M., Usuda S., Tanaka E., Tachibana K., Mishiro S., Machida A., Nakamura T., Miyakawa Y., Mayumi M. Hemagglutination assay of polypeptide coded by the pre-S region of hepatitis B virus DNA with monoclonal antibody: correlation of pre-S polypeptide with the receptor for polymerized human serum albumin in serums containing hepatitis B antigens. J Immunol. 1985 Feb;134(2):1212–1216. [PubMed] [Google Scholar]
  22. Pontisso P., Alberti A., Schiavon E., Tremolada F., Bortolotti F., Realdi G. Receptors for polymerized human serum albumin on hepatitis B virus particles detected by radioimmunoassay: changes in receptor activity in serum during acute and chronic infection. J Virol Methods. 1983 Mar;6(3):151–159. doi: 10.1016/0166-0934(83)90027-7. [DOI] [PubMed] [Google Scholar]
  23. Popper H., Shih J. W., Gerin J. L., Wong D. C., Hoyer B. H., London W. T., Sly D. L., Purcell R. H. Woodchuck hepatitis and hepatocellular carcinoma: correlation of histologic with virologic observations. Hepatology. 1981 Mar-Apr;1(2):91–98. doi: 10.1002/hep.1840010202. [DOI] [PubMed] [Google Scholar]
  24. Sanchez Y., Ionescu-Matiu I., Hollinger F. B., Melnick J. L., Dreesman G. R. Antigenic relationship of a hepatitis B surface antigen-derived polypeptide and human serum albumin. J Gen Virol. 1980 Jun;48(Pt 2):273–283. doi: 10.1099/0022-1317-48-2-273. [DOI] [PubMed] [Google Scholar]
  25. Schaeffer E., Sninsky J. J. Predicted secondary structure similarity in the absence of primary amino acid sequence homology: hepatitis B virus open reading frames. Proc Natl Acad Sci U S A. 1984 May;81(9):2902–2906. doi: 10.1073/pnas.81.9.2902. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Shih J. W., Cote P. J., Jr, Dapolito G. M., Gerin J. L. Production of monoclonal antibodies against hepatitis B surface antigen (HBsAg) by somatic cell hybrids. J Virol Methods. 1980;1(5):257–273. doi: 10.1016/0166-0934(80)90023-3. [DOI] [PubMed] [Google Scholar]
  27. Shih J. W., Tan P. L., Gerin J. L. Relationship of large hepatitis B surface antigen polypeptide to human serum albumin. Infect Immun. 1980 May;28(2):459–463. doi: 10.1128/iai.28.2.459-463.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Stibbe W., Gerlich W. H. Variable protein composition of hepatitis B surface antigen from different donors. Virology. 1982 Dec;123(2):436–442. doi: 10.1016/0042-6822(82)90275-6. [DOI] [PubMed] [Google Scholar]
  29. Summers J., Smolec J. M., Snyder R. A virus similar to human hepatitis B virus associated with hepatitis and hepatoma in woodchucks. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4533–4537. doi: 10.1073/pnas.75.9.4533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Thung S. N., Gerber M. A. Albumin binding sites of human hepatocytes. Liver. 1983 Oct;3(5):290–294. doi: 10.1111/j.1600-0676.1983.tb00880.x. [DOI] [PubMed] [Google Scholar]
  31. Thung S. N., Gerber M. A. Presence of receptors for polymerized albumin in HBsAg-containing hepatocytes and hepatoma cell line. Hepatology. 1981 Mar-Apr;1(2):132–136. doi: 10.1002/hep.1840010208. [DOI] [PubMed] [Google Scholar]
  32. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Trevisan A., Gudat F., Guggenheim R., Krey G., Dürmüller U., Lüond G., Düggelin M., Landmann J., Tondelli P., Bianchi L. Demonstration of albumin receptors on isolated human hepatocytes by light and scanning electron microscopy. Hepatology. 1982 Nov-Dec;2(6):832–835. doi: 10.1002/hep.1840020616. [DOI] [PubMed] [Google Scholar]
  34. Yu M. W., Finlayson J. S., Shih J. W. Interaction between various polymerized human albumins and hepatitis B surface antigen. J Virol. 1985 Sep;55(3):736–743. doi: 10.1128/jvi.55.3.736-743.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

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