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. 1988 Jun;62(6):2204–2208. doi: 10.1128/jvi.62.6.2204-2208.1988

Simian virus 40 (SV40) T antigen binds specifically to double-stranded DNA but not to single-stranded DNA or DNA/RNA hybrids containing the SV40 regulatory sequences.

K J Auborn 1, R B Markowitz 1, E Wang 1, Y T Yu 1, C Prives 1
PMCID: PMC253332  PMID: 3367427

Abstract

Simian virus 40 T antigen has been shown previously to bind specifically with high affinity to sites within the regulatory region of double-stranded simian virus 40 DNA. Using competition filter binding and the DNA-binding immunoassay, we show that T antigen did not bind specifically to either early or late single-stranded DNA containing these binding sites. Moreover, T antigen did not bind these sequences present in single-stranded RNA, RNA/RNA duplexes, or RNA/DNA hybrids. T antigen did, however, bind as efficiently to single-stranded DNA-cellulose as to double-stranded DNA-cellulose. This binding was nonspecific because it was independent of the presence of T-antigen-binding sites. The implications of these observations are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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