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. 1986 Dec;60(3):1050–1054. doi: 10.1128/jvi.60.3.1050-1054.1986

Selective shedding and congenital transmission of endogenous avian leukosis viruses.

E J Smith, D W Salter, R F Silva, L B Crittenden
PMCID: PMC253344  PMID: 3023657

Abstract

Shedding and congenital transmission of endogenous avian leukosis viruses were studied in viremic White Leghorn hens exogenously infected with viruses with endogenous long terminal repeats (LTRs) and in four semicongenic lines of hens that naturally express infectious endogenous viruses (EVs). Relatively high titers of infectious virus EV7 (encoded at locus ev7), Rous-associated virus-0 (RAV-0), and recombinant 882/-16 RAV-0 were detected in blood cells and sera from exogenously infected hens, but marked differences were noted in the incidence of congenitally infected progeny. In enzyme immunoassays that detect viral group-specific antigen, little or no p27 was detected in albumens from dams infected with RAV-0. However, hatchmates infected with either EV7 or recombinant 882/-16 RAV-0, which was constructed with an RAV-0 LTR, shed high titers of p27. Similarly, semicongenic hens that expressed RAV-0 (EV2) (encoded at locus ev2) shed little or no p27 into albumens, but hens that harbored ev10, ev11, and ev12 shed high titers of p27. A slower electrophoretic mobility of p27, considered to be characteristic of EVs that are restricted in congenital transmission, was not associated with low levels of shedding or congenital transmission; p27 from other EVs and p27 from an avian leukosis virus field strain, all of which are shed at high levels, had mobilities identical to that of p27 from RAV-0. Although shedding and congenital transmission appear to be controlled by the viral genome, there was no correlation between low efficiency of shedding or congenital transmission and endogenous LTR or p27 sequences.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Astrin S. M., Buss E. G., Haywards W. S. Endogenous viral genes are non-essential in the chicken. Nature. 1979 Nov 15;282(5736):339–341. doi: 10.1038/282339a0. [DOI] [PubMed] [Google Scholar]
  2. Astrin S. M. Endogenous viral genes of the White Leghorn chicken: common site of residence and sites associated with specific phenotypes of viral gene expression. Proc Natl Acad Sci U S A. 1978 Dec;75(12):5941–5945. doi: 10.1073/pnas.75.12.5941. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Coffin J. M., Tsichlis P. N., Conklin K. F., Senior A., Robinson H. L. Genomes of endogenous and exogenous avian retroviruses. Virology. 1983 Apr 15;126(1):51–72. doi: 10.1016/0042-6822(83)90461-0. [DOI] [PubMed] [Google Scholar]
  4. Crittenden L. B., Gulvas F. A., Eagen D. A. Spontaneous production and transmission of subgroup E retroviruses in line 15B chickens. Virology. 1980 Jun;103(2):400–406. doi: 10.1016/0042-6822(80)90199-3. [DOI] [PubMed] [Google Scholar]
  5. Crittenden L. B., Smith E. J. A comparison of test materials for differentiating avian leukosis virus group-specific antigens of exogenous and endogenous origin. Avian Dis. 1984 Oct-Dec;28(4):1057–1070. [PubMed] [Google Scholar]
  6. Crittenden L. B., Smith E. J., Fadly A. M. Influence of endogenous viral (ev) gene expression and strain of exogenous avian leukosis virus (ALV) on mortality and ALV infection and shedding in chickens. Avian Dis. 1984 Oct-Dec;28(4):1037–1056. [PubMed] [Google Scholar]
  7. Ewert D. L., Vainio O., Halpern M. S. Increased endogenous retroviral gene expression is a consequence of lymphocyte activation. J Immunol. 1983 Dec;131(6):3036–3041. [PubMed] [Google Scholar]
  8. Fadly A. M., Okazaki W., Smith E. J., Crittenden L. B. Relative efficiency of test procedures to detect lymphoid leukosis virus infection. Poult Sci. 1981 Sep;60(9):2037–2044. doi: 10.3382/ps.0602037. [DOI] [PubMed] [Google Scholar]
  9. HANAFUSA H., HANAFUSA T., RUBIN H. The defectiveness of Rous sarcoma virus. Proc Natl Acad Sci U S A. 1963 Apr;49:572–580. doi: 10.1073/pnas.49.4.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hughes S. H., Toyoshima K., Bishop J. M., Varmus H. E. Organization of the endogenous proviruses of chickens: implications for origin and expression. Virology. 1981 Jan 15;108(1):189–207. doi: 10.1016/0042-6822(81)90538-9. [DOI] [PubMed] [Google Scholar]
  11. Jurdic P., Huppert J., Greenland T., Heller E. Retroviral antigens on gs- chf- leukocytes. Nature. 1980 Nov 27;288(5789):400–401. doi: 10.1038/288400a0. [DOI] [PubMed] [Google Scholar]
  12. RUBIN H., CORNELIUS A., FANSHIER L. The pattern of congenital transmission of an avian lekosis virus. Proc Natl Acad Sci U S A. 1961 Jul 15;47:1058–1069. doi: 10.1073/pnas.47.7.1058. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Robinson H. L., Eisenman R. N. New findings on the congenital transmission of avian leukosis viruses. Science. 1984 Jul 27;225(4660):417–419. doi: 10.1126/science.6330893. [DOI] [PubMed] [Google Scholar]
  14. Robinson H. L., Eisenman R., Senior A., Ripley S. Low freqeuncy production of recombinant subgroup E avian leukosis viruses by uninfected V-15B chicken cells. Virology. 1979 Nov;99(1):21–30. doi: 10.1016/0042-6822(79)90033-3. [DOI] [PubMed] [Google Scholar]
  15. Rovigatti U. G., Astrin S. M. Avian endogenous viral genes. Curr Top Microbiol Immunol. 1983;103:1–21. doi: 10.1007/978-3-642-68943-7_1. [DOI] [PubMed] [Google Scholar]
  16. Silva R. F., Lee L. F. Monoclonal antibody-mediated immunoprecipitation of proteins from cells infected with Marek's disease virus or turkey herpesvirus. Virology. 1984 Jul 30;136(2):307–320. doi: 10.1016/0042-6822(84)90167-3. [DOI] [PubMed] [Google Scholar]
  17. Smith E. J., Fadly A., Okazaki W. An enzyme-linked immunosorbent assay for detecting avian leukosis-sarcoma viruses. Avian Dis. 1979 Jul-Sep;23(3):698–707. [PubMed] [Google Scholar]
  18. Vogt P. K., Friis R. R. An avian leukosis virus related to RSV(O): properties and evidence for helper activity. Virology. 1971 Jan;43(1):223–234. doi: 10.1016/0042-6822(71)90240-6. [DOI] [PubMed] [Google Scholar]
  19. Vogt V. M., Pepinsky R. B., Southard L. E. Primary structure of p19 species of avian sarcoma and leukemia viruses. J Virol. 1985 Oct;56(1):31–39. doi: 10.1128/jvi.56.1.31-39.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

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