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. 1988 Jul;62(7):2508–2511. doi: 10.1128/jvi.62.7.2508-2511.1988

Functional mapping of the genome of the B19 (human) parvovirus by in vitro translation after negative hybrid selection.

K Ozawa 1, J Ayub 1, N Young 1
PMCID: PMC253412  PMID: 3373576

Abstract

We have analyzed the coding capacity of B19 parvovirus transcripts by in vitro translation using the negative hybrid selection technique. Five different antisense oligonucleotides (18-mers) corresponding to different portions of the B19 genome were hybridized to RNA samples extracted from human erythroid bone marrow cells infected with B19 parvovirus in vitro, and RNase H was added to cleave specific B19 RNA molecules at selected sites. B19-specific translation products of these RNA samples were determined by immunoprecipitation. We localized the B19 nonstructural protein to the left-side transcript and the two capsid proteins to overlapping transcripts from the right side of the genome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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