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. 1988 Sep;62(9):3077–3083. doi: 10.1128/jvi.62.9.3077-3083.1988

Expression of interferon-induced genes in different tissues of mice.

A M Flenniken 1, J Galabru 1, M N Rutherford 1, A G Hovanessian 1, B R Williams 1
PMCID: PMC253423  PMID: 2969982

Abstract

In vivo responses to interferon (IFN) in mice were determined by measuring the steady-state levels of induced mRNAs following injection of IFN and poly(I)-poly(C). With cDNA probes for mouse 2'-5' oligoadenylate synthetase (2-5A synthetase) and 1-8, constitutive expression of the corresponding mRNA was detectable in different organs of normal C3H/He mice. These mRNA levels were increased by as much as 15-fold over control levels in various tissues, including the brain, after IFN and poly(I)-poly(C) treatment, coincident with increases in 2-5A synthetase enzyme activity. The basal activity level of this enzyme could be reduced in normal mice by treatment with anti-mouse IFN (alpha + beta) antibody. This treatment also reduced the levels of 2-5A synthetase and 1-8 mRNAs. Thus, physiological levels of circulating IFN maintain elevated levels of IFN-induced mRNAs in mice. Furthermore, changes in 2-5A synthetase enzyme activity reflect the changes in gene expression in vivo.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benech P., Merlin G., Revel M., Chebath J. 3' end structure of the human (2'-5') oligo A synthetase gene: prediction of two distinct proteins with cell type-specific expression. Nucleic Acids Res. 1985 Feb 25;13(4):1267–1281. doi: 10.1093/nar/13.4.1267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bocci V. Production and role of interferon in physiological conditions. Biol Rev Camb Philos Soc. 1981 Feb;56(1):49–85. doi: 10.1111/j.1469-185x.1981.tb00343.x. [DOI] [PubMed] [Google Scholar]
  3. Buffet-Janvresse C., Magard H., Robert N., Hovanessian A. G. Assay and the levels of 2-5A-synthetase in lymphocytes of patients with viral, bacterial and autoimmune diseases. Ann Immunol (Paris) 1983 Sep-Oct;134D(2):247–258. doi: 10.1016/s0771-050x(83)80090-4. [DOI] [PubMed] [Google Scholar]
  4. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  5. Clemens M. J., Williams B. R. Inhibition of cell-free protein synthesis by pppA2'p5'A2'p5'A: a novel oligonucleotide synthesized by interferon-treated L cell extracts. Cell. 1978 Mar;13(3):565–572. doi: 10.1016/0092-8674(78)90329-x. [DOI] [PubMed] [Google Scholar]
  6. Friedman R. L., Manly S. P., McMahon M., Kerr I. M., Stark G. R. Transcriptional and posttranscriptional regulation of interferon-induced gene expression in human cells. Cell. 1984 Oct;38(3):745–755. doi: 10.1016/0092-8674(84)90270-8. [DOI] [PubMed] [Google Scholar]
  7. Galabru J., Robert N., Buffet-Janvresse C., Rivière Y., Hovanessian A. G. Continuous production of interferon in normal mice: effect of anti-interferon globulin, sex, age, strain and environment on the levels of 2-5A synthetase and p67K kinase. J Gen Virol. 1985 Apr;66(Pt 4):711–718. doi: 10.1099/0022-1317-66-4-711. [DOI] [PubMed] [Google Scholar]
  8. Gresser I., Vignaux F., Belardelli F., Tovey M. G., Maunoury M. T. Injection of mice with antibody to mouse interferon alpha/beta decreases the level of 2'-5' oligoadenylate synthetase in peritoneal macrophages. J Virol. 1985 Jan;53(1):221–227. doi: 10.1128/jvi.53.1.221-227.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hannigan G., Williams B. R. Transcriptional regulation of interferon-responsive genes is closely linked to interferon receptor occupancy. EMBO J. 1986 Jul;5(7):1607–1613. doi: 10.1002/j.1460-2075.1986.tb04403.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hearl W. G., Johnston M. I. Accumulation of 2',5'-oligoadenylates in encephalomyocarditis virus-infected mice. J Virol. 1987 May;61(5):1586–1592. doi: 10.1128/jvi.61.5.1586-1592.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Higashi Y., Sokawa Y., Watanabe Y., Kawade Y., Ohno S., Takaoka C., Taniguchi T. Structure and expression of a cloned cDNA for mouse interferon-beta. J Biol Chem. 1983 Aug 10;258(15):9522–9529. [PubMed] [Google Scholar]
  12. Hovanessian A. G., Rivière Y., Krust B. Assay of interferon-mediated protein kinase activity from plasma and tissue extracts. Anal Biochem. 1983 Mar;129(2):349–356. doi: 10.1016/0003-2697(83)90561-4. [DOI] [PubMed] [Google Scholar]
  13. Ichii Y., Fukunaga R., Shiojiri S., Sokawa Y. Mouse 2-5A synthetase cDNA: nucleotide sequence and comparison to human 2-5A synthetase. Nucleic Acids Res. 1986 Dec 22;14(24):10117–10117. doi: 10.1093/nar/14.24.10117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Krust B., Rivière Y., Hovanessian A. G. p67K kinase in different tissues and plasma of control and interferon-treated mice. Virology. 1982 Jul 15;120(1):240–246. doi: 10.1016/0042-6822(82)90022-8. [DOI] [PubMed] [Google Scholar]
  15. Marcovistz R., Galabru J., Tsiang H., Hovanessian A. G. Neutralization of interferon produced early during rabies virus infection in mice. J Gen Virol. 1986 Feb;67(Pt 2):387–390. doi: 10.1099/0022-1317-67-2-387. [DOI] [PubMed] [Google Scholar]
  16. Raj N. B., Pitha P. M. Analysis of interferon mRNA in human fibroblast cells induced to produce interferon. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7426–7430. doi: 10.1073/pnas.78.12.7426. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Saunders M. E., Gewert D. R., Tugwell M. E., McMahon M., Williams B. R. Human 2-5A synthetase: characterization of a novel cDNA and corresponding gene structure. EMBO J. 1985 Jul;4(7):1761–1768. doi: 10.1002/j.1460-2075.1985.tb03848.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Saunders M., Gewert D., Castelino M., Rutherford M., Flenniken A., Willard H., Williams B. R. Characterization of the small 2-5A synthetase gene in human and mouse cells. Prog Clin Biol Res. 1985;202:163–174. [PubMed] [Google Scholar]
  19. Tovey M. G., Streuli M., Gresser I., Gugenheim J., Blanchard B., Guymarho J., Vignaux F., Gigou M. Interferon messenger RNA is produced constitutively in the organs of normal individuals. Proc Natl Acad Sci U S A. 1987 Jul;84(14):5038–5042. doi: 10.1073/pnas.84.14.5038. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Youn J. K., Hovanessian A. G., Riviere Y., Hue G., Lacour F. Enhancement of natural killer cell activity and 2-5A synthetase in mice treated with polyadenylic.polyuridylic acid. Cell Immunol. 1983 Jul 15;79(2):298–308. doi: 10.1016/0008-8749(83)90072-2. [DOI] [PubMed] [Google Scholar]

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