Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1988 Sep;62(9):3527–3529. doi: 10.1128/jvi.62.9.3527-3529.1988

Trypsin-sensitive neutralization site on VP1 of Theiler's murine encephalomyelitis viruses.

Y Ohara 1, A Senkowski 1, J L Fu 1, L Klaman 1, J Goodall 1, M Toth 1, R P Roos 1
PMCID: PMC253483  PMID: 2457118

Abstract

We generated Theiler's murine encephalomyelitis virus mutants resistant to several neutralizing monoclonal antibodies (MAbs) having their epitopes near a trypsin cleavage site of VP1. Neutralization and Western blot (immunoblot) studies suggest that two of the MAbs have identical epitopes that partly overlap the epitope of a third MAb. Sequencing of RNA of the mutants localized the epitopes to a site near the carboxyl end of VP1. The limited diversity of nucleotide changes seen in the mutants and the immunodominance of the site suggest that the carboxyl end of VP1 may have an important function.

Full text

PDF
3527

Images in this article

3527Image
on p.3527
3528Image
on p.3528

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Evans D. M., Minor P. D., Schild G. S., Almond J. W. Critical role of an eight-amino acid sequence of VP1 in neutralization of poliovirus type 3. Nature. 1983 Aug 4;304(5925):459–462. doi: 10.1038/304459a0. [DOI] [PubMed] [Google Scholar]
  2. Kousoulas K. G., Pellett P. E., Pereira L., Roizman B. Mutations affecting conformation or sequence of neutralizing epitopes identified by reactivity of viable plaques segregate from syn and ts domains of HSV-1(F) gB gene. Virology. 1984 Jun;135(2):379–394. doi: 10.1016/0042-6822(84)90194-6. [DOI] [PubMed] [Google Scholar]
  3. Lipton H. L. Persistent Theiler's murine encephalomyelitis virus infection in mice depends on plaque size. J Gen Virol. 1980 Jan;46(1):169–177. doi: 10.1099/0022-1317-46-1-169. [DOI] [PubMed] [Google Scholar]
  4. Lorch Y., Friedmann A., Lipton H. L., Kotler M. Theiler's murine encephalomyelitis virus group includes two distinct genetic subgroups that differ pathologically and biologically. J Virol. 1981 Nov;40(2):560–567. doi: 10.1128/jvi.40.2.560-567.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Nitayaphan S., Omilianowski D., Toth M. M., Parks G. D., Rueckert R. R., Palmenberg A. C., Roos R. P. Relationship of Theiler's murine encephalomyelitis viruses to the cardiovirus genus of picornaviruses. Intervirology. 1986;26(3):140–148. doi: 10.1159/000149693. [DOI] [PubMed] [Google Scholar]
  6. Nitayaphan S., Toth M. M., Roos R. P. Localization of a neutralization site of Theiler's murine encephalomyelitis viruses. J Virol. 1985 Dec;56(3):887–895. doi: 10.1128/jvi.56.3.887-895.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Nitayaphan S., Toth M. M., Roos R. P. Neutralizing monoclonal antibodies to Theiler's murine encephalomyelitis viruses. J Virol. 1985 Feb;53(2):651–657. doi: 10.1128/jvi.53.2.651-657.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ohara Y., Stein S., Fu J. L., Stillman L., Klaman L., Roos R. P. Molecular cloning and sequence determination of DA strain of Theiler's murine encephalomyelitis viruses. Virology. 1988 May;164(1):245–255. doi: 10.1016/0042-6822(88)90642-3. [DOI] [PubMed] [Google Scholar]
  9. Pevear D. C., Calenoff M., Rozhon E., Lipton H. L. Analysis of the complete nucleotide sequence of the picornavirus Theiler's murine encephalomyelitis virus indicates that it is closely related to cardioviruses. J Virol. 1987 May;61(5):1507–1516. doi: 10.1128/jvi.61.5.1507-1516.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES