Figure 4.

BHK-E1-G_CT cells contain elaborate arrays of tubular networks. BHK-E1-G_CT cells grown on 12-mm coverslips were processed for EM as described above. (A) A nest of smooth tubular membranes (TN) can be seen in the juxtanuclear region. The diameter of the tubules is ∼25–30 nm. The type R virus particles, which measure approximately 110 nm in diameter, have a round nucleoid with radial spokes and were most often observed in distended cisterna of the RER (arrowheads). Bar, 1 μm. nuc, nucleus. (B) Distended membrane profiles that may correspond to budding structures can be seen at the ends of tubules (arrowheads) at higher magnification. Bar, 0.1 μm.