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. 1988 Oct;62(10):3603–3607. doi: 10.1128/jvi.62.10.3603-3607.1988

Cytomegalovirus infection of peripheral blood mononuclear cells: effects on interleukin-1 and -2 production and responsiveness.

K Kapasi 1, G P Rice 1
PMCID: PMC253500  PMID: 2843662

Abstract

Cytomegalovirus suppresses the proliferative response of peripheral blood mononuclear cells to phytohemagglutinin. In these experiments, we identified which mononuclear cell subpopulation might be responsible for the suppression. We found that prior infection of either lymphocytes or monocytes followed by reconstitution with monocytes or lymphocytes, respectively, would abrogate the proliferative response in a subsequent culture with phytohemagglutinin. Infection of either cell type also reduced both the production of interleukin-1 (IL-1) and IL-2 and the proliferative response to exogenously supplied IL-1 or IL-2. We did not find evidence for an IL-2 antagonist. These experiments suggest that cytomegalovirus causes a metabolic derangement in lymphocytes and monocytes and impairs their ability both to produce and to respond to physiological mediators of the immune response.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beck S., Barrell B. G. Human cytomegalovirus encodes a glycoprotein homologous to MHC class-I antigens. Nature. 1988 Jan 21;331(6153):269–272. doi: 10.1038/331269a0. [DOI] [PubMed] [Google Scholar]
  2. Braun R. W., Reiser H. C. Replication of human cytomegalovirus in human peripheral blood T cells. J Virol. 1986 Oct;60(1):29–36. doi: 10.1128/jvi.60.1.29-36.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carney W. P., Hirsch M. S. Mechanisms of immunosuppression in cytomegalovirus mononucleosis. II. Virus-monocyte interactions. J Infect Dis. 1981 Jul;144(1):47–54. doi: 10.1093/infdis/144.1.47. [DOI] [PubMed] [Google Scholar]
  4. Carney W. P., Rubin R. H., Hoffman R. A., Hansen W. P., Healey K., Hirsch M. S. Analysis of T lymphocyte subsets in cytomegalovirus mononucleosis. J Immunol. 1981 Jun;126(6):2114–2116. [PubMed] [Google Scholar]
  5. Drew W. L., Mintz L. What is the role of cytomegalovirus in AIDS? Ann N Y Acad Sci. 1984;437:320–324. doi: 10.1111/j.1749-6632.1984.tb37151.x. [DOI] [PubMed] [Google Scholar]
  6. Dudding L. R., Garnett H. M. Interaction of strain AD169 and a clinical isolate of cytomegalovirus with peripheral monocytes: the effect of lipopolysaccharide stimulation. J Infect Dis. 1987 May;155(5):891–896. doi: 10.1093/infdis/155.5.891. [DOI] [PubMed] [Google Scholar]
  7. Durum S. K., Schmidt J. A., Oppenheim J. J. Interleukin 1: an immunological perspective. Annu Rev Immunol. 1985;3:263–287. doi: 10.1146/annurev.iy.03.040185.001403. [DOI] [PubMed] [Google Scholar]
  8. Dutko F. J., Oldstone M. B. Cytomegalovirus causes a latent infection in undifferentiated cells and is activated by induction of cell differentiation. J Exp Med. 1981 Nov 1;154(5):1636–1651. doi: 10.1084/jem.154.5.1636. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Einhorn L., Ost A. Cytomegalovirus infection of human blood cells. J Infect Dis. 1984 Feb;149(2):207–214. doi: 10.1093/infdis/149.2.207. [DOI] [PubMed] [Google Scholar]
  10. Felsenstein D., Carney W. P., Iacoviello V. R., Hirsch M. S. Phenotypic properties of atypical lymphocytes in cytomegalovirus-induced mononucleosis. J Infect Dis. 1985 Jul;152(1):198–203. doi: 10.1093/infdis/152.1.198. [DOI] [PubMed] [Google Scholar]
  11. Griffiths P. D., Grundy J. E. Molecular biology and immunology of cytomegalovirus. Biochem J. 1987 Jan 15;241(2):313–324. doi: 10.1042/bj2410313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hirsch M. S., Felsenstein D. Cytomegalovirus-induced immunosuppression. Ann N Y Acad Sci. 1984;437:8–15. doi: 10.1111/j.1749-6632.1984.tb37117.x. [DOI] [PubMed] [Google Scholar]
  13. Ho D. D., Pomerantz R. J., Kaplan J. C. Pathogenesis of infection with human immunodeficiency virus. N Engl J Med. 1987 Jul 30;317(5):278–286. doi: 10.1056/NEJM198707303170505. [DOI] [PubMed] [Google Scholar]
  14. Kapasi K., Rice G. P. Role of the monocyte in cytomegalovirus-mediated immunosuppression in vitro. J Infect Dis. 1986 Nov;154(5):881–884. doi: 10.1093/infdis/154.5.881. [DOI] [PubMed] [Google Scholar]
  15. Loh L., Hudson J. B. Murine cytomegalovirus-induced immunosuppression. Infect Immun. 1982 Apr;36(1):89–95. doi: 10.1128/iai.36.1.89-95.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rice G. P., Schrier R. D., Oldstone M. B. Cytomegalovirus infects human lymphocytes and monocytes: virus expression is restricted to immediate-early gene products. Proc Natl Acad Sci U S A. 1984 Oct;81(19):6134–6138. doi: 10.1073/pnas.81.19.6134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rodgers B. C., Scott D. M., Mundin J., Sissons J. G. Monocyte-derived inhibitor of interleukin 1 induced by human cytomegalovirus. J Virol. 1985 Sep;55(3):527–532. doi: 10.1128/jvi.55.3.527-532.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schrier R. D., Nelson J. A., Oldstone M. B. Detection of human cytomegalovirus in peripheral blood lymphocytes in a natural infection. Science. 1985 Nov 29;230(4729):1048–1051. doi: 10.1126/science.2997930. [DOI] [PubMed] [Google Scholar]
  19. Schrier R. D., Rice G. P., Oldstone M. B. Suppression of natural killer cell activity and T cell proliferation by fresh isolates of human cytomegalovirus. J Infect Dis. 1986 Jun;153(6):1084–1091. doi: 10.1093/infdis/153.6.1084. [DOI] [PubMed] [Google Scholar]
  20. Sing G. K., Garnett H. M. The effects of human cytomegalovirus challenge in vitro on subpopulations of T cells from seronegative donors. J Med Virol. 1984;14(4):363–371. doi: 10.1002/jmv.1890140409. [DOI] [PubMed] [Google Scholar]
  21. Wahren B., Ljungman P., Paulin T., Ringdén O. Enhancive and suppressive effects of cytomegalovirus on human lymphocyte responses in vitro. J Virol. 1986 Jun;58(3):909–913. doi: 10.1128/jvi.58.3.909-913.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Yam L. T., Li C. Y., Crosby W. H. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol. 1971 Mar;55(3):283–290. doi: 10.1093/ajcp/55.3.283. [DOI] [PubMed] [Google Scholar]

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